Restriction map for pF1KF0120
##################################
# NO CUT ENZYMES (   55 enzymes) #
##################################

AatII       AclI        AflII       AocI        AscI        
AseI        AsnI        Asp700I     AspI        AviII       
AvrII       BamHI       BciVI       BfrI        BlnI        
BsiWI       BsrDI       Bst1107I    BstZ17I     Bsu36I      
CpoI        DraI        DraIII      EagI        EclXI       
Eco52I      Eco81I      EcoRI       FseI        FspI        
HpaI        KspI        MfeI        MunI        NdeI        
NheI        NotI        PacI        PflFI       PmeI        
PshBI       Psp1406I    PvuI        RsrII       SacII       
SexAI       SgrAI       SnaBI       SpeI        SphI        
SspI        SwaI        Tth111I     XbaI        XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

Acc65I       2270  GGTACC               G^GTACC
AccIII       2603  TCCGGA               T^CCGGA
AcsI          286  GAATTT               [GA]^AATT[CT]
AgeI          701  ACCGGT               A^CCGGT
AhdI         1404  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      2628  AGCGCT               AGC^GCT
ApoI          286  GAATTT               [GA]^AATT[CT]
Asp718I      2270  GGTACC               G^GTACC
AspEI        1404  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI         1307  GGCGCC               GGCGC^C
BbrPI        1972  CACGTG               CAC^GTG
BbvCI        2871  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1751  GCAGCACAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII        1797  AGATCT               A^GATCT
BlpI           96  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       96  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1972  CACGTG               [CT]AC^GT[GA]
BsaBI        1248  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI        2603  TCCGGA               T^CCGGA
BsiEI         921  CGACCG               CG[GA][CT]^CG
BsmI         3103  GAATGC               GAATGC(1/-1)
BspDI         685  ATCGAT               AT^CGAT
BspEI        2603  TCCGGA               T^CCGGA
BspHI         656  TCATGA               T^CATGA
BspLU11I     3368  ACATGT               A^CATGT
BssHII       2447  GCGCGC               G^CGCGC
BstBI        3100  TTCGAA               TT^CGAA
BstEII       1956  GGTCACC              G^GT[ACGT]ACC
BstPI        1956  GGTCACC              G^GT[ACGT]ACC
CelII          96  GCTCAGC              GC^T[ACGT]AGC
ClaI          685  ATCGAT               AT^CGAT
Eam1105I     1404  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     2628  AGCGCT               AGC^GCT
EcoO65I      1956  GGTCACC              G^GT[ACGT]ACC
EcoRV        3019  GATATC               GAT^ATC
EcoT22I      3105  ATGCAT               ATGCA^T
HindIII      2676  AAGCTT               A^AGCTT
KasI         1307  GGCGCC               G^GCGCC
KpnI         2270  GGTACC               GGTAC^C
MamI         1248  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         3095  ACGCGT               A^CGCGT
MroI         2603  TCCGGA               T^CCGGA
NarI         1307  GGCGCC               GG^CGCC
NcoI         2993  CCATGG               C^CATGG
NruI         2668  TCGCGA               TCG^CGA
NsiI         3105  ATGCAT               ATGCA^T
NspV         3100  TTCGAA               TT^CGAA
PaeR7I        679  CTCGAG               C^TCGAG
PflMI        2472  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         701  ACCGGT               A^CCGGT
PmaCI        1972  CACGTG               CAC^GTG
PmlI         1972  CACGTG               CAC^GTG
PshAI        1336  GACCAGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          656  TCATGA               T^CATGA
SacI         1042  GAGCTC               GAGCT^C
SalI         2455  GTCGAC               G^TCGAC
SbfI          975  CCTGCAGG             CCTGCA^GG
SfiI          367  GGCCACGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1307  GGCGCC               GGC^GCC
SfuI         3100  TTCGAA               TT^CGAA
Sse8387I      975  CCTGCAGG             CCTGCA^GG
Tsp509I       287  AATT                 ^AATT
Van91I       2472  CCATGACCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          347  CCACCATTGTGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          679  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         2455  GTCGAC               GT^[AC][GT]AC
             2572  GTAGAC              
AflIII       3095  ACGCGT               A^C[GA][CT]GT
             3368  ACATGT              
Alw44I       2074  GTGCAC               G^TGCAC
             2401  GTGCAC              
ApaI         1980  GGGCCC               GGGCC^C
             1987  GGGCCC              
ApaLI        2074  GTGCAC               G^TGCAC
             2401  GTGCAC              
BclI         2306  TGATCA               T^GATCA
             3191  TGATCA              
BglI          368  GCCACGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              988  GCCCGCCAGGC         
BsaI          668  GAGACC               GGTCTC(1/5)
             3061  GAGACC              
Bsp1407I      305  TGTACA               T^GTACA
             1079  TGTACA              
BsrGI         305  TGTACA               T^GTACA
             1079  TGTACA              
BstAPI       2967  GCAGAGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3150  GCACTGCCTGC         
DsaI         2993  CCATGG               C^C[GA][CT]GG
             3353  CCACGG              
FbaI         2306  TGATCA               T^GATCA
             3191  TGATCA              
MaeII        1973  ACGT                 A^CGT
             2405  ACGT                
MseI          182  TTAA                 T^TAA
             1189  TTAA                
PpuMI         434  AGGACCT              [GA]G^G[AT]CC[CT]
             1034  AGGACCT             
ScaI         1175  AGTACT               AGT^ACT
             1463  AGTACT              
SmaI         1701  CCCGGG               CCC^GGG
             2648  CCCGGG              
SmlI          679  CTCGAG               C^T[CT][GA]AG
             1045  CTCAAG              
SspBI         305  TGTACA               T^GTACA
             1079  TGTACA              
StuI         1142  AGGCCT               AGG^CCT
             1274  AGGCCT              
TfiI          341  GATTC                G^A[AT]TC
             1426  GATTC               
Tru9I         182  TTAA                 T^TAA
             1189  TTAA                
XmaCI        1701  CCCGGG               C^CCGGG
             2648  CCCGGG              
XmaI         1701  CCCGGG               C^CCGGG
             2648  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  113 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbsI        BbvI        
BcnI        BfaI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BsaWI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BssSI       BstF5I      
BstNI       BstUI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DrdI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PleI        PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XhoII       
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