Restriction map for pF1KF0120
 (Linear) (Six-base) MAPSORT of: FLJ00120.seq  Check: 7625  from: 1  to: 3462



FLJ00120.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:20  ..

AccI GT'mk_AC

Cuts at:      0    2456    2573    3462 
   Size:       2456     117     889

  Fragments arranged by size: 

                2456     889     117
 
Acc65I G'GTAC_C

Cuts at:      0    2270    3462 
   Size:       2270    1192
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     778     847    1472    1525    1567    2404    2683 
   Size:         64     714      69     625      53      42     837     279

Cuts at:   2683    3334    3462 
   Size:        651     128

  Fragments arranged by size: 

                 837     714     651     625     279     128      69      64
                  53      42
 
AfeI AGC'GCT

Cuts at:      0    2630    3462 
   Size:       2630     832
 
AflIII A'CryG_T

Cuts at:      0    3095    3368    3462 
   Size:       3095     273      94

  Fragments arranged by size: 

                3095     273      94
 
AgeI A'CCGG_T

Cuts at:      0     701    3462 
   Size:        701    2761
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1409    3462 
   Size:       1409    2053
 
AleI CACnn'nnGTG

Cuts at:      0     352    1963    2408    3462 
   Size:        352    1611     445    1054

  Fragments arranged by size: 

                1611    1054     445     352
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1534    3462 
   Size:       1534    1928
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    3277    3309    3462 
   Size:       3277      32     153

  Fragments arranged by size: 

                3277     153      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     798     846     954    1344    2136    2544    2973    3462 
   Size:        798      48     108     390     792     408     429     489

  Fragments arranged by size: 

                 798     792     489     429     408     390     108      48
            
 
ApaI G_GGCC'C

Cuts at:      0    1984    1991    3462 
   Size:       1984       7    1471

  Fragments arranged by size: 

                1984    1471       7
 
ApaLI G'TGCA_C

Cuts at:      0    2074    2401    3462 
   Size:       2074     327    1061

  Fragments arranged by size: 

                2074    1061     327
 
ApoI r'AATT_y

Cuts at:      0     286    3462 
   Size:        286    3176
 
AvaI C'yCGr_G

Cuts at:      0     679    1154    1701    2648    2766    2982    3077    3462 
   Size:        679     475     547     947     118     216      95     385

  Fragments arranged by size: 

                 947     679     547     475     385     216     118      95
            
 
BanI G'GyrC_C

Cuts at:      0    1307    2270    2488    2578    2989    3462 
   Size:       1307     963     218      90     411     473

  Fragments arranged by size: 

                1307     963     473     411     218      90
 
BanII G_rGCy'C

Cuts at:      0     332    1046    1700    1984    1991    2514    3462 
   Size:        332     714     654     284       7     523     948

  Fragments arranged by size: 

                 948     714     654     523     332     284       7
 
BbeI G_GCGC'C

Cuts at:      0    1311    3462 
   Size:       1311    2151
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     261     835    1460    1772    3061    3462 
   Size:        261     574     625     312    1289     401

  Fragments arranged by size: 

                1289     625     574     401     312     261
 
BbvCI CC'TCA_GC

Cuts at:      0    2872    3462 
   Size:       2872     590
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1740    1774    3462 
   Size:       1740      34    1688

  Fragments arranged by size: 

                1740    1688      34
 
BclI T'GATC_A

Cuts at:      0    2306    3191    3462 
   Size:       2306     885     271

  Fragments arranged by size: 

                2306     885     271
 
BfrBI ATG'CAT

Cuts at:      0    3107    3462 
   Size:       3107     355
 
BglI GCCn_nnn'nGGC

Cuts at:      0     374     994    3462 
   Size:        374     620    2468

  Fragments arranged by size: 

                2468     620     374
 
BglII A'GATC_T

Cuts at:      0    1797    3462 
   Size:       1797    1665
 
BlpI GC'TnA_GC

Cuts at:      0      97    3462 
   Size:         97    3365
 
Bme1580I G_kGCm'C

Cuts at:      0    1580    1973    1984    1991    2078    2405    2491    2601 
   Size:       1580     393      11       7      87     327      86     110

Cuts at:   2601    3462 
   Size:        861

  Fragments arranged by size: 

                1580     861     393     327     110      87      86      11
                   7
 
BmgBI CAC'GTC

Cuts at:      0    2406    3462 
   Size:       2406    1056
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     329    3462 
   Size:        329    3133
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496     754     826     958    1042    1060    1135    1249 
   Size:        496     258      72     132      84      18      75     114

Cuts at:   1249    1312    1918    2337    3181    3349    3462 
   Size:         63     606     419     844     168     113

  Fragments arranged by size: 

                 844     606     496     419     258     168     132     114
                 113      84      75      72      63      18
 
Bpu10I CC'TnA_GC

Cuts at:      0    2872    3462 
   Size:       2872     590
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1030    3462 
   Size:       1030    2432
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     662    3055    3462 
   Size:        662    2393     407

  Fragments arranged by size: 

                2393     662     407
 
BsaAI yAC'GTr

Cuts at:      0    1974    3462 
   Size:       1974    1488
 
BsaBI GATnn'nnATC

Cuts at:      0    1252    3462 
   Size:       1252    2210
 
BsaHI Gr'CG_yC

Cuts at:      0      59    1308    1598    1790    2366    2807    3462 
   Size:         59    1249     290     192     576     441     655

  Fragments arranged by size: 

                1249     655     576     441     290     192      59
 
BsaWI w'CCGG_w

Cuts at:      0     701    2273    2603    3462 
   Size:        701    1572     330     859

  Fragments arranged by size: 

                1572     859     701     330
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     835     865    1033    1063    1840    1870    3277    3307 
   Size:        835      30     168      30     777      30    1407      30

Cuts at:   3307    3462 
   Size:        155

  Fragments arranged by size: 

                1407     835     777     168     155      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      23     652     675     733     997    1015    1039    1132 
   Size:         23     629      23      58     264      18      24      93

Cuts at:   1132    1246    1456    1830    1842    1845    1848    1851    2512 
   Size:        114     210     374      12       3       3       3     661

Cuts at:   2512    2581    3301    3304    3400    3436    3462 
   Size:         69     720       3      96      36      26

  Fragments arranged by size: 

                 720     661     629     374     264     210     114      96
                  93      69      58      36      26      24      23      23
                  18      12       3       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1487    2033    2046    2497    2513    2859    3166    3462 
   Size:       1487     546      13     451      16     346     307     296

  Fragments arranged by size: 

                1487     546     451     346     307     296      16      13
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2017    2230    2247    2355    2986    3162    3462 
   Size:       2017     213      17     108     631     176     300

  Fragments arranged by size: 

                2017     631     300     213     176     108      17
 
BsiEI CG_ry'CG

Cuts at:      0     924    3462 
   Size:        924    2538
 
BsiHKAI G_wGCw'C

Cuts at:      0    1046    1054    2078    2405    2933    3221    3462 
   Size:       1046       8    1024     327     528     288     241

  Fragments arranged by size: 

                1046    1024     528     327     288     241       8
 
BsmI GAATG_Cn'

Cuts at:      0    3109    3462 
   Size:       3109     353
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      31    2668    3274    3462 
   Size:         31    2637     606     188

  Fragments arranged by size: 

                2637     606     188      31
 
Bsp1286I G_dGCh'C

Cuts at:      0     332    1046    1054    1580    1700    1973    1984    1991 
   Size:        332     714       8     526     120     273      11       7

Cuts at:   1991    2078    2405    2491    2514    2601    2933    3221    3462 
   Size:         87     327      86      23      87     332     288     241

  Fragments arranged by size: 

                 714     526     332     332     327     288     273     241
                 120      87      87      86      23      11       8       7
            
 
BspEI T'CCGG_A

Cuts at:      0    2603    3462 
   Size:       2603     859
 
BspHI T'CATG_A

Cuts at:      0     656    3462 
   Size:        656    2806
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     143     446     783     983    1364    1893    2702    3462 
   Size:        143     303     337     200     381     529     809     760

  Fragments arranged by size: 

                 809     760     529     381     337     303     200     143
            
 
BsrBI CCG'CTC

Cuts at:      0     534     558     810    1128    1134    2751    3462 
   Size:        534      24     252     318       6    1617     711

  Fragments arranged by size: 

                1617     711     534     318     252      24       6
 
BsrFI r'CCGG_y

Cuts at:      0      86     221     701    2110    2921    3308    3462 
   Size:         86     135     480    1409     811     387     154

  Fragments arranged by size: 

                1409     811     480     387     154     135      86
 
BsrGI T'GTAC_A

Cuts at:      0     305    1079    3462 
   Size:        305     774    2383

  Fragments arranged by size: 

                2383     774     305
 
BssHII G'CGCG_C

Cuts at:      0    2447    3462 
   Size:       2447    1015
 
BssSI C'ACGA_G

Cuts at:      0     370    1579    2434    3462 
   Size:        370    1209     855    1028

  Fragments arranged by size: 

                1209    1028     855     370
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2973    3156    3462 
   Size:       2973     183     306

  Fragments arranged by size: 

                2973     306     183
 
BstBI TT'CG_AA

Cuts at:      0    3101    3462 
   Size:       3101     361
 
BstEII G'GTnAC_C

Cuts at:      0    1956    3462 
   Size:       1956    1506
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     354     357    2361    2949    2961    3444    3462 
   Size:        354       3    2004     588      12     483      18

  Fragments arranged by size: 

                2004     588     483     354      18      12       3
 
BstYI r'GATC_y

Cuts at:      0     965    1217    1618    1797    3462 
   Size:        965     252     401     179    1665

  Fragments arranged by size: 

                1665     965     401     252     179
 
BtgI C'CryG_G

Cuts at:      0    2993    3353    3462 
   Size:       2993     360     109

  Fragments arranged by size: 

                2993     360     109
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1732    3093    3462 
   Size:       1732    1361     369

  Fragments arranged by size: 

                1732    1361     369
 
BtsI GCAGTG_nn'

Cuts at:      0    1189    3150    3462 
   Size:       1189    1961     312

  Fragments arranged by size: 

                1961    1189     312
 
ClaI AT'CG_AT

Cuts at:      0     686    3462 
   Size:        686    2776
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     580    1717    2650    3462 
   Size:        580    1137     933     812

  Fragments arranged by size: 

                1137     933     812     580
 
EaeI y'GGCC_r

Cuts at:      0     458    2099    2688    2951    3134    3462 
   Size:        458    1641     589     263     183     328

  Fragments arranged by size: 

                1641     589     458     328     263     183
 
EarI CTCTTCn'nnn_

Cuts at:      0      47     548    2831    2938    3226    3462 
   Size:         47     501    2283     107     288     236

  Fragments arranged by size: 

                2283     501     288     236     107      47
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1472    3462 
   Size:       1472    1990
 
EcoICRI GAG'CTC

Cuts at:      0    1044    3462 
   Size:       1044    2418
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64     496     754     778     826     847     958    1042 
   Size:         64     432     258      24      48      21     111      84

Cuts at:   1042    1060    1135    1249    1312    1472    1525    1567    1918 
   Size:         18      75     114      63     160      53      42     351

Cuts at:   1918    2337    2404    2683    3181    3334    3349    3462 
   Size:        419      67     279     498     153      15     113

  Fragments arranged by size: 

                 498     432     419     351     279     258     160     153
                 114     113     111      84      75      67      64      63
                  53      48      42      24      21      18      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     979    1063    1315    1900    2119    3160    3462 
   Size:        979      84     252     585     219    1041     302

  Fragments arranged by size: 

                1041     979     585     302     252     219      84
 
EcoO109I rG'GnC_Cy

Cuts at:      0     375     435     933    1035    1878    1980    1987    1988 
   Size:        375      60     498     102     843     102       7       1

Cuts at:   1988    3333    3462 
   Size:       1345     129

  Fragments arranged by size: 

                1345     843     498     375     129     102     102      60
                   7       1
 
EcoRV GAT'ATC

Cuts at:      0    3021    3462 
   Size:       3021     441
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2647    3462 
   Size:       2647     815
 
HaeII r_GCGC'y

Cuts at:      0    1311    2544    2632    2919    3462 
   Size:       1311    1233      88     287     543

  Fragments arranged by size: 

                1311    1233     543     287      88
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     835     867    1395    1427    3176    3208    3462 
   Size:        835      32     528      32    1749      32     254

  Fragments arranged by size: 

                1749     835     528     254      32      32      32
 
HincII GTy'rAC

Cuts at:      0     960    1287    1908    2259    2457    3462 
   Size:        960     327     621     351     198    1005

  Fragments arranged by size: 

                1005     960     621     351     327     198
 
HindIII A'AGCT_T

Cuts at:      0    2676    3462 
   Size:       2676     786
 
KasI G'GCGC_C

Cuts at:      0    1307    3462 
   Size:       1307    2155
 
KpnI G_GTAC'C

Cuts at:      0    2274    3462 
   Size:       2274    1188
 
MluI A'CGCG_T

Cuts at:      0    3095    3462 
   Size:       3095     367
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     699    2162    3224    3462 
   Size:        209     490    1463    1062     238

  Fragments arranged by size: 

                1463    1062     490     238     209
 
MscI TGG'CCA

Cuts at:      0     460    2690    2953    3462 
   Size:        460    2230     263     509

  Fragments arranged by size: 

                2230     509     460     263
 
MslI CAynn'nnrTG

Cuts at:      0     352     355    1102    1648    1963    2408    3229    3442 
   Size:        352       3     747     546     315     445     821     213

Cuts at:   3442    3462 
   Size:         20

  Fragments arranged by size: 

                 821     747     546     445     352     315     213      20
                   3
 
MspA1I CmG'CkG

Cuts at:      0     474    1113    1518    1778    2133    2231    2361    3459 
   Size:        474     639     405     260     355      98     130    1098

Cuts at:   3459    3462 
   Size:          3

  Fragments arranged by size: 

                1098     639     474     405     355     260     130      98
                   3
 
NaeI GCC'GGC

Cuts at:      0     223    2112    2923    3310    3462 
   Size:        223    1889     811     387     152

  Fragments arranged by size: 

                1889     811     387     223     152
 
NarI GG'CG_CC

Cuts at:      0    1308    3462 
   Size:       1308    2154
 
NcoI C'CATG_G

Cuts at:      0    2993    3462 
   Size:       2993     469
 
NgoMIV G'CCGG_C

Cuts at:      0     221    2110    2921    3308    3462 
   Size:        221    1889     811     387     154

  Fragments arranged by size: 

                1889     811     387     221     154
 
NruI TCG'CGA

Cuts at:      0    2670    3462 
   Size:       2670     792
 
NsiI A_TGCA'T

Cuts at:      0    3109    3462 
   Size:       3109     353
 
NspI r_CATG'y

Cuts at:      0      96    2192    3372    3462 
   Size:         96    2096    1180      90

  Fragments arranged by size: 

                2096    1180      96      90
 
PasI CC'CwG_GG

Cuts at:      0    1059    1984    3462 
   Size:       1059     925    1478

  Fragments arranged by size: 

                1478    1059     925
 
PciI A'CATG_T

Cuts at:      0    3368    3462 
   Size:       3368      94
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2478    3462 
   Size:       2478     984
 
PfoI T'CCnGG_A

Cuts at:      0     527     902    3462 
   Size:        527     375    2560

  Fragments arranged by size: 

                2560     527     375
 
PmlI CAC'GTG

Cuts at:      0    1974    3462 
   Size:       1974    1488
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3277    3309    3462 
   Size:       3277      32     153

  Fragments arranged by size: 

                3277     153      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     435    1035    3462 
   Size:        435     600    2427

  Fragments arranged by size: 

                2427     600     435
 
PshAI GACnn'nnGTC

Cuts at:      0    1340    3462 
   Size:       1340    2122
 
PspOMI G'GGCC_C

Cuts at:      0    1980    1987    3462 
   Size:       1980       7    1475

  Fragments arranged by size: 

                1980    1475       7
 
PstI C_TGCA'G

Cuts at:      0     416     851     980    1112    1523    2540    2618    3462 
   Size:        416     435     129     132     411    1017      78     844

  Fragments arranged by size: 

                1017     844     435     416     411     132     129      78
            
 
PvuII CAG'CTG

Cuts at:      0     474    1113    1518    2133    2361    3459    3462 
   Size:        474     639     405     615     228    1098       3

  Fragments arranged by size: 

                1098     639     615     474     405     228       3
 
SacI G_AGCT'C

Cuts at:      0    1046    3462 
   Size:       1046    2416
 
SalI G'TCGA_C

Cuts at:      0    2455    3462 
   Size:       2455    1007
 
SapI GCTCTTCn'nnn_

Cuts at:      0     548    2831    2938    3226    3462 
   Size:        548    2283     107     288     236

  Fragments arranged by size: 

                2283     548     288     236     107
 
SbfI CC_TGCA'GG

Cuts at:      0     980    3462 
   Size:        980    2482
 
ScaI AGT'ACT

Cuts at:      0    1177    1465    3462 
   Size:       1177     288    1997

  Fragments arranged by size: 

                1997    1177     288
 
SfcI C'TryA_G

Cuts at:      0     412     847     976    1108    1519    2502    2536    2614 
   Size:        412     435     129     132     411     983      34      78

Cuts at:   2614    2886    3462 
   Size:        272     576

  Fragments arranged by size: 

                 983     576     435     412     411     272     132     129
                  78      34
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     374    3462 
   Size:        374    3088
 
SfoI GGC'GCC

Cuts at:      0    1309    3462 
   Size:       1309    2153
 
SmaI CCC'GGG

Cuts at:      0    1703    2650    3462 
   Size:       1703     947     812

  Fragments arranged by size: 

                1703     947     812
 
SmlI C'TyrA_G

Cuts at:      0     679    1045    3462 
   Size:        679     366    2417

  Fragments arranged by size: 

                2417     679     366
 
StuI AGG'CCT

Cuts at:      0    1144    1276    3462 
   Size:       1144     132    2186

  Fragments arranged by size: 

                2186    1144     132
 
StyI C'CwwG_G

Cuts at:      0     245     431    1316    1571    2993    3462 
   Size:        245     186     885     255    1422     469

  Fragments arranged by size: 

                1422     885     469     255     245     186
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1944    2070    2596    2955    3432    3462 
   Size:       1944     126     526     359     477      30

  Fragments arranged by size: 

                1944     526     477     359     126      30
 
TatI w'GTAC_w

Cuts at:      0     305    1079    1175    1205    1463    3462 
   Size:        305     774      96      30     258    1999

  Fragments arranged by size: 

                1999     774     305     258      96      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81    1033    1065    1840    1872    3462 
   Size:         49      32     952      32     775      32    1590

  Fragments arranged by size: 

                1590     952     775      49      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     354    3462 
   Size:        354    3108
 
XhoI C'TCGA_G

Cuts at:      0     679    3462 
   Size:        679    2783
 
XmaI C'CCGG_G

Cuts at:      0    1701    2648    3462 
   Size:       1701     947     814

  Fragments arranged by size: 

                1701     947     814
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI     DrdI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI    HaeII    Hin4I   HincII  HindIII     KasI     KpnI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NruI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmlI
     PpiI    PpuMI    PshAI   PspOMI     PstI    PvuII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SmaI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BciVI     BmtI     BplI    BsiWI    BsrDI  BstZ17I
   Bsu36I    CspCI     DraI   DraIII     EagI    EcoRI     FseI     FspI
    FspAI     HpaI     MfeI     NdeI     NheI     NotI     PacI     PmeI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII    SanDI    SexAI
     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XmnI     ZraI
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