Restriction map for pF1KA1121
##################################
# NO CUT ENZYMES (   61 enzymes) #
##################################

Acc65I      AflII       Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspI        AvrII       BamHI       BbrPI       BfrI        
BlnI        BsaBI       BsaI        BsiWI       BspDI       
BssSI       Bst1107I    BstAPI      BstEII      BstPI       
BstZ17I     ClaI        DraI        DraIII      Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     KpnI        
MamI        MluI        NdeI        NheI        NotI        
NruI        NsiI        PacI        PaeR7I      PflFI       
PmaCI       PmeI        PmlI        PshBI       PvuI        
SbfI        ScaI        SfiI        SmlI        SnaBI       
Sse8387I    SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII         938  GACGTC               GACGT^C
AccIII       3953  TCCGGA               T^CCGGA
AclI         2330  AACGTT               AA^CGTT
AgeI          473  ACCGGT               A^CCGGT
AhdI            7  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI         1696  CCTCAGG              CC^T[ACGT]AGG
AspEI           7  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         656  TGCGCA               TGC^GCA
BcgI         2176  CGACATCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          245  GCCGCGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          699  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      699  GCTGAGC              GC^T[ACGT]AGC
BsaAI        3697  TACGTG               [CT]AC^GT[GA]
BseAI        3953  TCCGGA               T^CCGGA
BsmI         1569  GCATTC               GAATGC(1/-1)
BspEI        3953  TCCGGA               T^CCGGA
BspHI         527  TCATGA               T^CATGA
BsrDI        2894  GCAATG               GCAATG(2/0)
BstBI         856  TTCGAA               TT^CGAA
Bsu36I       1696  CCTCAGG              CC^T[ACGT]AGG
CelII         699  GCTGAGC              GC^T[ACGT]AGC
CpoI         3821  CGGACCG              CG^G[AT]CCG
DrdI         2769  GACGTTCCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I        7  GACCCTTCGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       1696  CCTCAGG              CC^T[ACGT]AGG
EcoNI         681  CCTCAGCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI          259  GGCCGGCC             GGCCGG^CC
FspI          656  TGCGCA               TGC^GCA
HindIII      2632  AAGCTT               A^AGCTT
HpaI         3097  GTTAAC               GTT^AAC
KspI          751  CCGCGG               CCGC^GG
MroI         3953  TCCGGA               T^CCGGA
NcoI         2280  CCATGG               C^CATGG
NspV          856  TTCGAA               TT^CGAA
PflMI         504  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         473  ACCGGT               A^CCGGT
Psp1406I     2330  AACGTT               AA^CGTT
RcaI          527  TCATGA               T^CATGA
RsrII        3821  CGGACCG              CG^G[AT]CCG
SacII         751  CCGCGG               CCGC^GG
SalI         2411  GTCGAC               G^TCGAC
SapI          564  GAAGAGC              GCTCTTC(1/4)
SexAI        3811  ACCTGGT              A^CC[AT]GGT
SfuI          856  TTCGAA               TT^CGAA
SgrAI         156  CGCCGGCG             C[GA]^CCGG[CT]G
SpeI         2661  ACTAGT               A^CTAGT
Van91I        504  CCAGATCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AlwNI         451  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             2854  CAGCTGCTG           
Asp700I      1380  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2671  GAAGTTCTTC          
AvaI          620  CCCGGG               C^[CT]CG[GA]G
             3673  CCCGGG              
BbvCI         681  CCTCAGC              CCTCAGC(-5/-2)
             1936  CCTCAGC             
BciVI         991  GTATCC               GTATCC(6/5)
             1075  GTATCC              
BclI         1299  TGATCA               T^GATCA
             3774  TGATCA              
BglII        1518  AGATCT               A^GATCT
             3195  AGATCT              
BsmBI        3961  CGTCTC               CGTCTC(1/5)
             2767  GAGACG              
BsoBI         620  CCCGGG               C^[CT]CG[GA]G
             3673  CCCGGG              
Bsp1407I     1013  TGTACA               T^GTACA
             3755  TGTACA              
BspLU11I     1010  ACATGT               A^CATGT
             2709  ACATGT              
BsrGI        1013  TGTACA               T^GTACA
             3755  TGTACA              
BssHII         89  GCGCGC               G^CGCGC
               91  GCGCGC              
EagI          125  CGGCCG               C^GGCCG
              258  CGGCCG              
EclXI         125  CGGCCG               C^GGCCG
              258  CGGCCG              
Eco52I        125  CGGCCG               C^GGCCG
              258  CGGCCG              
FbaI         1299  TGATCA               T^GATCA
             3774  TGATCA              
MfeI         1711  CAATTG               C^AATTG
             2972  CAATTG              
MunI         1711  CAATTG               C^AATTG
             2972  CAATTG              
PshAI        1896  GACCCAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3702  GACTTTCGTC          
SacI          702  GAGCTC               GAGCT^C
             1976  GAGCTC              
SmaI          620  CCCGGG               CCC^GGG
             3673  CCCGGG              
SphI         1067  GCATGC               GCATG^C
             2760  GCATGC              
SspBI        1013  TGTACA               T^GTACA
             3755  TGTACA              
StuI         1224  AGGCCT               AGG^CCT
             2290  AGGCCT              
XmaCI         620  CCCGGG               C^CCGGG
             3673  CCCGGG              
XmaI          620  CCCGGG               C^CCGGG
             3673  CCCGGG              
XmnI         1380  GAAGCTGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2671  GAAGTTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccI        AccII       AciI        AcsI        AcyI        
AfaI        AflIII      AluI        AlwI        ApoI        
AspHI       AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaJI       
BsaWI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        KasI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SfoI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XcmI        XhoII       
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