Restriction map for pF1KE0153
##################################
# NO CUT ENZYMES (  170 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     AspEI       
AspI        AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsiYI       BslI        BsmBI       
BsmFI       BsmI        BsoBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstEII      BstF5I      BstNI       
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRII      EcoT22I     
FbaI        FokI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HpaI        
HphI        KasI        KspI        MaeI        MaeII       
MfeI        MluI        MroI        MslI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SgrAI       SmaI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I        710  GGTACC               G^GTACC
AlwNI         731  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I       710  GGTACC               G^GTACC
AspHI         718  GAGCTC               G[AT]GC[AT]^C
AvaII         244  GGTCC                G^G[AT]CC
BalI          927  TGGCCA               TGG^CCA
BanI          710  GGTACC               G^G[CT][GA]CC
BanII         718  GAGCTC               G[GA]GC[CT]^C
BcnI           28  CCCGG                CC^[GC]GG
BmyI          718  GAGCTC               G[AGT]GC[ACT]^C
BsaBI         154  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI         225  CCTTGG               C^C[ACGT][ACGT]GG
BsiHKAI       718  GAGCTC               G[AT]GC[AT]^C
Bsp1286I      718  GAGCTC               G[AGT]GC[ACT]^C
BssKI          28  CCCGG                ^CC[ACGT]GG
BstBI         110  TTCGAA               TT^CGAA
BstXI         587  CCAGAGAATTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         885  GGATCT               [GA]^GATC[CT]
CfoI           21  GCGC                 GCG^C
EaeI          927  TGGCCA               [CT]^GGCC[GA]
EarI          960  GAAGAG               CTCTTC(1/4)
EcoRV         253  GATATC               GAT^ATC
EcoT14I       225  CCTTGG               C^C[AT][AT]GG
HapII          29  CCGG                 C^CGG
HhaI           21  GCGC                 GCG^C
HinP1I         21  GCGC                 G^CGC
HpaII          29  CCGG                 C^CGG
KpnI          710  GGTACC               GGTAC^C
Ksp632I       960  GAAGAG               CTCTTC(1/4)
MamI          154  GATTTCTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          885  GGATCT               [GA]^GATC[CT]
MluNI         927  TGGCCA               TGG^CCA
MscI          927  TGGCCA               TGG^CCA
MspI           29  CCGG                 C^CGG
NciI           28  CCCGG                CC^[GC]GG
NspV          110  TTCGAA               TT^CGAA
PstI          488  CTGCAG               CTGCA^G
SacI          718  GAGCTC               GAGCT^C
ScrFI          28  CCCGG                CC^[ACGT]GG
SfcI          488  CTGCAG               C^T[GA][CT]AG
SfuI          110  TTCGAA               TT^CGAA
SmlI          742  CTCAAG               C^T[CT][GA]AG
StuI          294  AGGCCT               AGG^CCT
StyI          225  CCTTGG               C^C[AT][AT]GG
TaqI          111  TCGA                 T^CGA
TthHB8I       111  TCGA                 T^CGA
XhoII         885  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

BpmI           99  CTCCAG               CTGGAG(16/14)
              315  CTGGAG              
BseRI         784  GAGGAG               GAGGAG(10/8)
              988  GAGGAG              
BsgI          671  GTGCAG               GTGCAG(16/14)
              832  GTGCAG              
BsmAI         789  GAGAC                GTCTC(1/5)
               97  GTCTC               
DdeI          338  CTGAG                C^T[ACGT]AG
              374  CTCAG               
FauI           14  GCGGG                CCCGC(4/6)
               32  GCGGG               
HindIII       136  AAGCTT               A^AGCTT
              797  AAGCTT              
MaeIII        104  GTGAC                ^GT[ACGT]AC
              652  GTGAC               
MseI          305  TTAA                 T^TAA
              518  TTAA                
MwoI           14  GCGGGAAGCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               23  GCAGGCCCGGC         
NlaIV          39  GGGGCC               GG[ACGT]^[ACGT]CC
              710  GGTACC              
SfaNI         733  GATGC                GCATC(5/9)
              993  GCATC               
Tru9I         305  TTAA                 T^TAA
              518  TTAA                
Tsp45I        104  GTGAC                ^GT[GC]AC
              652  GTGAC               


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BbvI        BsrI        Cac8I       Cfr13I      
DpnII       Fnu4HI      HaeIII      HinfI       ItaI        
MboI        MboII       MnlI        NdeII       NlaIII      
RsaI        Sau3AI      Sau96I      TfiI        TseI        
Tsp509I     TspRI       
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