Restriction map for pF1KE0441
##################################
# NO CUT ENZYMES (  183 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BalI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
BpmI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiWI       BsmAI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DraI        DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
HpaII       HphI        KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AciI          123  GCGG                 CCGC(-3/-1)
AcsI          256  GAATTT               [GA]^AATT[CT]
AocI          605  CCTCAGG              CC^T[ACGT]AGG
ApoI          256  GAATTT               [GA]^AATT[CT]
BamHI         513  GGATCC               G^GATCC
BanI          483  GGCACC               G^G[CT][GA]CC
BbsI          474  GAAGAC               GAAGAC(2/6)
BglI            7  GCCAAGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         474  GAAGAC               GAAGAC(2/6)
BspMI         535  ACCTGC               ACCTGC(4/8)
BssKI          63  CCTGG                ^CC[ACGT]GG
BstAPI        454  GCACCCTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          63  CCTGG                CC^[AT]GG
BstXI         419  CCAAGAAAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        605  CCTCAGG              CC^T[ACGT]AGG
Cac8I         669  GCCTGC               GC[ACGT]^[ACGT]GC
EaeI            5  CGGCCA               [CT]^GGCC[GA]
Eco81I        605  CCTCAGG              CC^T[ACGT]AGG
EcoRII         63  CCTGG                ^CC[AT]GG
HindIII       337  AAGCTT               A^AGCTT
MvaI           63  CCTGG                CC^[AT]GG
SacI          184  GAGCTC               GAGCT^C
ScrFI          63  CCTGG                CC^[ACGT]GG
SmlI          187  CTCAAG               C^T[CT][GA]AG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AvaII         150  GGACC                G^G[AT]CC
              533  GGACC               
BanII         184  GAGCTC               G[GA]GC[CT]^C
              504  GGGCTC              
BbvI          366  GCAGC                GCAGC(8/12)
              539  GCTGC               
BseRI         202  GAGGAG               GAGGAG(10/8)
              628  GAGGAG              
BsiYI         649  CCTTCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              684  CCAGACTGAGG         
BslI          649  CCTTCTGTGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              684  CCAGACTGAGG         
BsmFI         532  GGGAC                GGGAC(10/14)
              681  GTCCC               
BstF5I        294  GGATG                GGATG(2/0)
              429  GGATG               
BstYI         222  GGATCT               [GA]^GATC[CT]
              513  GGATCC              
DpnII         223  GATC                 ^GATC
              514  GATC                
DraII         149  AGGACCT              [GA]G^G[ACGT]CC[CT]
              532  GGGACCT             
DraIII        455  CACCCTGTG            CAC[ACGT][ACGT][ACGT]^GTG
              661  CACGCTGTG           
EcoO109I      149  AGGACCT              [GA]G^G[ACGT]CC[CT]
              532  GGGACCT             
Fnu4HI        366  GCAGC                GC^[ACGT]GC
              539  GCTGC               
FokI          294  GGATG                GGATG(9/13)
              429  GGATG               
HinfI         393  GACTC                G^A[ACGT]TC
              443  GAGTC               
ItaI          366  GCAGC                GC^[ACGT]GC
              539  GCTGC               
MaeIII        142  GTGAC                ^GT[ACGT]AC
              637  GTGAC               
MboI          223  GATC                 ^GATC
              514  GATC                
MflI          222  GGATCT               [GA]^GATC[CT]
              513  GGATCC              
MseI          110  TTAA                 T^TAA
              313  TTAA                
MslI           32  CACATCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              523  CATCACTGTG          
NdeII         223  GATC                 ^GATC
              514  GATC                
PleI          393  GACTC                GAGTC(4/5)
              443  GAGTC               
PpuMI         149  AGGACCT              [GA]G^G[AT]CC[CT]
              532  GGGACCT             
Sau3AI        223  GATC                 ^GATC
              514  GATC                
StuI          602  AGGCCT               AGG^CCT
              609  AGGCCT              
Tru9I         110  TTAA                 T^TAA
              313  TTAA                
TseI          366  GCAGC                G^C[AT]GC
              539  GCTGC               
Tsp45I        142  GTGAC                ^GT[GC]AC
              637  GTGAC               
XhoII         222  GGATCT               [GA]^GATC[CT]
              513  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   20 enzymes) #
#########################################

AluI        AlwI        AspHI       BmyI        BsiHKAI     
Bsp1286I    BsrI        Cfr13I      DdeI        EarI        
HaeIII      Ksp632I     MboII       MnlI        MwoI        
NlaIV       SapI        Sau96I      Tsp509I     TspRI       
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