Restriction map for pF1KB8907
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AvaI        AviII       
AvrII       BalI        BamHI       BbrPI       BbvCI       
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BsaAI       BsaBI       BsaI        BsaWI       BseAI       
BsgI        BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspEI       BspHI       BspLU11I    
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       CpoI        
DraI        DraIII      EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HindIII     HpaI        KpnI        
Ksp632I     MamI        MfeI        MluI        MluNI       
MroI        MscI        MseI        MunI        NaeI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp509I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccII          85  CGCG                 CG^CG
AlwI          225  GATCC                GGATC(4/5)
AlwNI         232  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          477  GGGCCC               GGGCC^C
AspI          418  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI          121  GGCGCC               G^G[CT][GA]CC
BbeI          121  GGCGCC               GGCGC^C
BbsI           63  GAAGAC               GAAGAC(2/6)
BglI          338  GCCTGACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI          63  GAAGAC               GAAGAC(2/6)
BseRI         283  GAGGAG               GAGGAG(10/8)
BsiEI         408  CGACCG               CG[GA][CT]^CG
BsmAI         424  GTCTC                GTCTC(1/5)
BspDI         214  ATCGAT               AT^CGAT
BsrDI         307  CATTGC               GCAATG(2/0)
BstEII        240  GGTGACC              G^GT[ACGT]ACC
BstPI         240  GGTGACC              G^GT[ACGT]ACC
BstUI          85  CGCG                 CG^CG
BstXI         458  CCAGCGAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         224  AGATCC               [GA]^GATC[CT]
ClaI          214  ATCGAT               AT^CGAT
DraII         476  AGGGCCC              [GA]G^G[ACGT]CC[CT]
DrdI            9  GACCGTGGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          153  CGGCCA               [CT]^GGCC[GA]
EcoO109I      476  AGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       240  GGTGACC              G^GT[ACGT]ACC
HincII        297  GTTGAC               GT[CT]^[GA]AC
HindII        297  GTTGAC               GT[CT]^[GA]AC
KasI          121  GGCGCC               G^GCGCC
KspI           84  CCGCGG               CCGC^GG
MflI          224  AGATCC               [GA]^GATC[CT]
MslI          213  CATCGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           85  CGCG                 CG^CG
NarI          121  GGCGCC               GG^CGCC
NlaIII        177  CATG                 CATG^
PflFI         418  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         156  CCACGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          229  CTGCAG               CTGCA^G
SacII          84  CCGCGG               CCGC^GG
SfaNI         257  GCATC                GCATC(5/9)
SfcI          229  CTGCAG               C^T[GA][CT]AG
SfoI          121  GGCGCC               GGC^GCC
Tth111I       418  GACAGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        156  CCACGACTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         224  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AvaII           8  GGACC                G^G[AT]CC
              625  GGACC               
BfaI          146  CTAG                 C^TAG
              574  CTAG                
BspMI          32  ACCTGC               ACCTGC(4/8)
              203  ACCTGC              
BsrBI         360  CCGCTC               CCGCTC(-3/-3)
              286  GAGCGG              
BsrFI         151  ACCGGC               [GA]^CCGG[CT]
              410  ACCGGC              
Cfr10I        151  ACCGGC               [GA]^CCGG[CT]
              410  ACCGGC              
DdeI          548  CTGAG                C^T[ACGT]AG
              632  CTGAG               
DpnII         174  GATC                 ^GATC
              225  GATC                
DsaI           11  CCGTGG               C^C[GA][CT]GG
               84  CCGCGG              
HaeII         121  GGCGCC               [GA]GCGC^[CT]
              180  GGCGCT              
HapII         152  CCGG                 C^CGG
              411  CCGG                
HgaI           24  GACGC                GACGC(5/10)
              142  GCGTC               
HinfI          16  GAGTC                G^A[ACGT]TC
              634  GAGTC               
HpaII         152  CCGG                 C^CGG
              411  CCGG                
MaeI          146  CTAG                 C^TAG
              574  CTAG                
MaeII         295  ACGT                 A^CGT
              495  ACGT                
MboI          174  GATC                 ^GATC
              225  GATC                
MspI          152  CCGG                 C^CGG
              411  CCGG                
NdeII         174  GATC                 ^GATC
              225  GATC                
PleI           16  GAGTC                GAGTC(4/5)
              634  GAGTC               
PvuII         235  CAGCTG               CAG^CTG
              375  CAGCTG              
Sau3AI        174  GATC                 ^GATC
              225  GATC                
SmlI          363  CTCAAG               C^T[CT][GA]AG
              582  CTTGAG              
XcmI          404  CCACCGACCGGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              542  CCAGGACTGAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AcyI        AluI        BanII       BbvI        
BmyI        BsaHI       BsaJI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HhaI        
Hin1I       HinP1I      HphI        ItaI        MaeIII      
MboII       MnlI        MspA1I      MvaI        MwoI        
NlaIV       Sau96I      ScrFI       TaqI        TseI        
Tsp45I      TspRI       TthHB8I     
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