Restriction map for pF1KB6331
##################################
# NO CUT ENZYMES (  177 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BanII       
BbeI        BbrPI       BbsI        BcgI        BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     CelII       CfoI        ClaI        CpoI        
DpnII       DraI        DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MaeII       MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StyI        SwaI        TfiI        Tru9I       Tsp45I      
Tth111I     Van91I      XbaI        XhoII       XmaCI       
XmaI        XmnI        


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# ONE CUT ENZYMES (   35 enzymes) #
###################################

AlwNI         148  CAGGCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          747  CCTTAGG              CC^T[ACGT]AGG
AspHI         205  GTGCTC               G[AT]GC[AT]^C
AvaI          449  CTCGAG               C^[CT]CG[GA]G
AvaII          31  GGACC                G^G[AT]CC
BanI          524  GGCACC               G^G[CT][GA]CC
BciVI         191  GGATAC               GTATCC(6/5)
BcnI          686  CCCGG                CC^[GC]GG
BmyI          205  GTGCTC               G[AGT]GC[ACT]^C
BsiHKAI       205  GTGCTC               G[AT]GC[AT]^C
BsoBI         449  CTCGAG               C^[CT]CG[GA]G
Bsp1286I      205  GTGCTC               G[AGT]GC[ACT]^C
BsrDI         105  CATTGC               GCAATG(2/0)
BsrFI         275  ACCGGC               [GA]^CCGG[CT]
BsrI          162  CCAGT                ACTGG(1/-1)
BssSI          83  CTCGTG               CACGAG(-5/-1)
Bsu36I        747  CCTTAGG              CC^T[ACGT]AGG
Cfr10I        275  ACCGGC               [GA]^CCGG[CT]
DraII         149  AGGCCCT              [GA]G^G[ACGT]CC[CT]
Eco81I        747  CCTTAGG              CC^T[ACGT]AGG
EcoNI         387  CCTTTCTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      149  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HinfI         484  GACTC                G^A[ACGT]TC
MslI          504  CATCCTAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          686  CCCGG                CC^[GC]GG
NlaIV         524  GGCACC               GG[ACGT]^[ACGT]CC
NspI          569  GCATGT               [GA]CATG^[CT]
PaeR7I        449  CTCGAG               C^TCGAG
PleI          484  GACTC                GAGTC(4/5)
PstI          219  CTGCAG               CTGCA^G
TaqI          450  TCGA                 T^CGA
Tsp509I       756  AATT                 ^AATT
TthHB8I       450  TCGA                 T^CGA
XcmI          470  CCAGGCATGGCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          449  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BbvCI          18  CCTCAGC              CCTCAGC(-5/-2)
              252  GCTGAGG             
BpmI          331  CTGGAG               CTGGAG(16/14)
              666  CTGGAG              
BseRI          16  CTCCTC               GAGGAG(10/8)
              705  CTCCTC              
BsmAI         695  GAGAC                GTCTC(1/5)
              779  GTCTC               
BsmFI         230  GGGAC                GGGAC(10/14)
              377  GGGAC               
EarI           42  CTCTTC               CTCTTC(1/4)
              423  CTCTTC              
HphI          763  GGTGA                GGTGA(8/7)
              211  TCACC               
Ksp632I        42  CTCTTC               CTCTTC(1/4)
              423  CTCTTC              
MboII          43  TCTTC                GAAGA(8/7)
              424  TCTTC               
SfcI          219  CTGCAG               C^T[GA][CT]AG
              294  CTACAG              
SmlI          449  CTCGAG               C^T[CT][GA]AG
              592  CTCAAG              
StuI          335  AGGCCT               AGG^CCT
              722  AGGCCT              
TspRI         126  CACTG                CA[GC]TG[ACGT][ACGT]^
              203  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   33 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BsaJI       
BsgI        BsiYI       BslI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HpaII       
ItaI        MnlI        MspA1I      MspI        MvaI        
MwoI        NlaIII      PvuII       RsaI        Sau96I      
ScrFI       SfaNI       TseI        
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