Restriction map for pF1KB6331
 (Linear) (Six-base) MAPSORT of: KIBB6331.seq  Check: 4383  from: 1  to: 783



KIBB6331.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:46  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     612     783 
   Size:        600      12     171

  Fragments arranged by size: 

                 600     171      12
 
AlwNI CAG_nnn'CTG

Cuts at:      0     153     783 
   Size:        153     630
 
AvaI C'yCGr_G

Cuts at:      0     449     783 
   Size:        449     334
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     104     137     148     181     783 
   Size:        104      33      11      33     602

  Fragments arranged by size: 

                 602     104      33      33      11
 
BanI G'GyrC_C

Cuts at:      0     524     783 
   Size:        524     259
 
BbvCI CC'TCA_GC

Cuts at:      0      19     253     783 
   Size:         19     234     530

  Fragments arranged by size: 

                 530     234      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     185     783 
   Size:        185     598
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     156     783 
   Size:        156     627
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      27     718     783 
   Size:         27     691      65

  Fragments arranged by size: 

                 691      65      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     687     783 
   Size:        352     335      96

  Fragments arranged by size: 

                 352     335      96
 
Bpu10I CC'TnA_GC

Cuts at:      0      19     146     253     783 
   Size:         19     127     107     530

  Fragments arranged by size: 

                 530     127     107      19
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     577     783 
   Size:        577     206
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     688     718     783 
   Size:        688      30      65

  Fragments arranged by size: 

                 688      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0       7     696     783 
   Size:          7     689      87

  Fragments arranged by size: 

                 689      87       7
 
BseYI C'CCAG_C

Cuts at:      0     141     236     528     690     783 
   Size:        141      95     292     162      93

  Fragments arranged by size: 

                 292     162     141      95      93
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     249     332     376     783 
   Size:        249      83      44     407

  Fragments arranged by size: 

                 407     249      83      44
 
BsiHKAI G_wGCw'C

Cuts at:      0     209     783 
   Size:        209     574
 
Bsp1286I G_dGCh'C

Cuts at:      0     209     783 
   Size:        209     574
 
BsrDI GCAATG_nn'

Cuts at:      0     104     783 
   Size:        104     679
 
BsrFI r'CCGG_y

Cuts at:      0     275     783 
   Size:        275     508
 
BssSI C'ACGA_G

Cuts at:      0      83     783 
   Size:         83     700
 
Bsu36I CC'TnA_GG

Cuts at:      0     748     783 
   Size:        748      35
 
EarI CTCTTCn'nnn_

Cuts at:      0      48     429     783 
   Size:         48     381     354

  Fragments arranged by size: 

                 381     354      48
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      39     273     783 
   Size:         39     234     510

  Fragments arranged by size: 

                 510     234      39
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     600     612     687     783 
   Size:        352     248      12      75      96

  Fragments arranged by size: 

                 352     248      96      75      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     391     783 
   Size:        391     392
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     783 
   Size:        150     633
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     630     783 
   Size:        630     153
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      23      55     688     720     783 
   Size:         23      32     633      32      63

  Fragments arranged by size: 

                 633      63      32      32      23
 
MslI CAynn'nnrTG

Cuts at:      0     508     783 
   Size:        508     275
 
MspA1I CmG'CkG

Cuts at:      0     330     632     716     783 
   Size:        330     302      84      67

  Fragments arranged by size: 

                 330     302      84      67
 
NspI r_CATG'y

Cuts at:      0     573     783 
   Size:        573     210
 
PstI C_TGCA'G

Cuts at:      0     223     783 
   Size:        223     560
 
PvuII CAG'CTG

Cuts at:      0     330     632     716     783 
   Size:        330     302      84      67

  Fragments arranged by size: 

                 330     302      84      67
 
SfcI C'TryA_G

Cuts at:      0     219     294     783 
   Size:        219      75     489

  Fragments arranged by size: 

                 489     219      75
 
SmlI C'TyrA_G

Cuts at:      0     449     592     783 
   Size:        449     143     191

  Fragments arranged by size: 

                 449     191     143
 
StuI AGG'CCT

Cuts at:      0     337     724     783 
   Size:        337     387      59

  Fragments arranged by size: 

                 387     337      59
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     542     783 
   Size:        250     292     241

  Fragments arranged by size: 

                 292     250     241
 
TatI w'GTAC_w

Cuts at:      0     641     783 
   Size:        641     142
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     477     783 
   Size:        477     306
 
XhoI C'TCGA_G

Cuts at:      0     449     783 
   Size:        449     334
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BaeI     BanI    BbvCI    BciVI     BmrI
     BplI     BpmI   Bpu10I    BpuEI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI Bsp1286I    BsrDI    BsrFI    BssSI   Bsu36I     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    Hin4I     MslI   MspA1I     NspI
     PstI    PvuII     SfcI     SmlI     StuI    TaqII     TatI     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII    BamHI    BanII     BbeI     BbsI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StyI     SwaI     TstI  Tth111I     XbaI
     XmaI     XmnI     ZraI
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