Restriction map for pF1KB7340
##################################
# NO CUT ENZYMES (   77 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       BfrI        BglI        BglII       BsiWI       
Bsp1407I    BspDI       BspHI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        CpoI        
DrdI        Eam1105I    Eco81I      EcoO65I     EcoRV       
FseI        FspI        KpnI        MfeI        MluI        
MunI        NcoI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsrII       
SalI        SbfI        ScaI        SfiI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AflIII       3447  ACATGT               A^C[GA][CT]GT
Aor51HI       940  AGCGCT               AGC^GCT
Asp700I       915  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         867  CCTAGG               C^CTAGG
BbeI           67  GGCGCC               GGCGC^C
BbrPI         389  CACGTG               CAC^GTG
BbsI          285  GAAGAC               GAAGAC(2/6)
BciVI        1734  GGATAC               GTATCC(6/5)
BclI         1422  TGATCA               T^GATCA
BlnI          867  CCTAGG               C^CTAGG
BpuAI         285  GAAGAC               GAAGAC(2/6)
BsaAI         389  CACGTG               [CT]AC^GT[GA]
BsaBI         487  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI         1363  GCATTC               GAATGC(1/-1)
BspLU11I     3447  ACATGT               A^CATGT
BspMI        1059  ACCTGC               ACCTGC(4/8)
BsrBI          74  CCGCTC               CCGCTC(-3/-3)
DsaI          141  CCGCGG               C^C[GA][CT]GG
EagI          233  CGGCCG               C^GGCCG
EclXI         233  CGGCCG               C^GGCCG
Eco47III      940  AGCGCT               AGC^GCT
Eco52I        233  CGGCCG               C^GGCCG
EcoNI         974  CCTGCAAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      2098  ATGCAT               ATGCA^T
FbaI         1422  TGATCA               T^GATCA
HincII       2841  GTTAAC               GT[CT]^[GA]AC
HindII       2841  GTTAAC               GT[CT]^[GA]AC
HindIII      1973  AAGCTT               A^AGCTT
HpaI         2841  GTTAAC               GTT^AAC
KasI           67  GGCGCC               G^GCGCC
KspI          141  CCGCGG               CCGC^GG
MamI          487  GATGAGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI           67  GGCGCC               GG^CGCC
NdeI         3434  CATATG               CA^TATG
NheI         3217  GCTAGC               G^CTAGC
NotI          232  GCGGCCGC             GC^GGCCGC
NsiI         2098  ATGCAT               ATGCA^T
PmaCI         389  CACGTG               CAC^GTG
PmlI          389  CACGTG               CAC^GTG
PshAI        1848  GACATCAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII         141  CCGCGG               CCGC^GG
SexAI        2826  ACCAGGT              A^CC[AT]GGT
SfoI           67  GGCGCC               GGC^GCC
StuI          172  AGGCCT               AGG^CCT
XmnI          915  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AccII         131  CGCG                 CG^CG
              142  CGCG                
AccIII        768  TCCGGA               T^CCGGA
              892  TCCGGA              
AspHI         690  GAGCTC               G[AT]GC[AT]^C
             1791  GAGCTC              
BalI          275  TGGCCA               TGG^CCA
             3186  TGGCCA              
BbvCI        1933  CCTCAGC              CCTCAGC(-5/-2)
             2600  CCTCAGC             
BcgI          992  CGATTCCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              188  GCATCTTCGTCG        
BlpI         1063  GCTAAGC              GC^T[ACGT]AGC
             2881  GCTGAGC             
Bpu1102I     1063  GCTAAGC              GC^T[ACGT]AGC
             2881  GCTGAGC             
BseAI         768  TCCGGA               T^CCGGA
              892  TCCGGA              
BsiEI         105  CGGTCG               CG[GA][CT]^CG
              233  CGGCCG              
BsiHKAI       690  GAGCTC               G[AT]GC[AT]^C
             1791  GAGCTC              
BsmBI         680  CGTCTC               CGTCTC(1/5)
              587  GAGACG              
BspEI         768  TCCGGA               T^CCGGA
              892  TCCGGA              
BstUI         131  CGCG                 CG^CG
              142  CGCG                
CelII        1063  GCTAAGC              GC^T[ACGT]AGC
             2881  GCTGAGC             
DraI         2289  TTTAAA               TTT^AAA
             2670  TTTAAA              
DraIII        386  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2804  CACTGAGTG           
EcoRI        1644  GAATTC               G^AATTC
             3288  GAATTC              
HgaI           91  GACGC                GACGC(5/10)
              679  GCGTC               
MluNI         275  TGGCCA               TGG^CCA
             3186  TGGCCA              
MroI          768  TCCGGA               T^CCGGA
              892  TCCGGA              
MscI          275  TGGCCA               TGG^CCA
             3186  TGGCCA              
MvnI          131  CGCG                 CG^CG
              142  CGCG                
SacI          690  GAGCTC               GAGCT^C
             1791  GAGCTC              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApaI        ApoI        
AvaI        AvaII       BamHI       BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrDI       BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        Hin1I       
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NaeI        
NciI        NdeII       NgoMIV      NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       PstI        
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XcmI        XhoII       
XmaCI       XmaI        
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