Restriction map for pF1KB6821
##################################
# NO CUT ENZYMES (  191 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwI        AlwNI       AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BbeI        BbrPI       BbsI        BbvCI       BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BpmI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmFI       BsoBI       
Bsp1407I    BspDI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DpnII       DraIII      DrdI        DsaI        
EaeI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoT14I     
EcoT22I     FauI        FbaI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        HphI        KasI        KpnI        
KspI        MaeI        MamI        MboI        MfeI        
MflI        MluI        MluNI       MscI        MslI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau3AI      SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TfiI        Tsp45I      Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccIII        164  TCCGGA               T^CCGGA
AciI           66  CCGC                 CCGC(-3/-1)
AfaI          448  GTAC                 GT^AC
ApaI           31  GGGCCC               GGGCC^C
AvaII         152  GGACC                G^G[AT]CC
BbvI          172  GCAGC                GCAGC(8/12)
BcgI           53  GCATGATGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI         164  TCCGGA               [AT]^CCGG[AT]
BseAI         164  TCCGGA               T^CCGGA
BsmI          160  GCATTC               GAATGC(1/-1)
BspEI         164  TCCGGA               T^CCGGA
BssKI          43  CCTGG                ^CC[ACGT]GG
BstAPI         68  GCAACGATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI          43  CCTGG                CC^[AT]GG
Cac8I         415  GCAGGC               GC[ACGT]^[ACGT]GC
DraI          118  TTTAAA               TTT^AAA
DraII          30  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoNI         409  CCTCTTGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I       30  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRII         43  CCTGG                ^CC[AT]GG
EcoRV         367  GATATC               GAT^ATC
Fnu4HI        172  GCAGC                GC^[ACGT]GC
HaeII         305  AGCGCC               [GA]GCGC^[CT]
HaeIII         32  GGCC                 GG^CC
HapII         165  CCGG                 C^CGG
HpaII         165  CCGG                 C^CGG
ItaI          172  GCAGC                GC^[ACGT]GC
MaeII         219  ACGT                 A^CGT
MaeIII        456  GTTAC                ^GT[ACGT]AC
MroI          164  TCCGGA               T^CCGGA
MseI          119  TTAA                 T^TAA
MspA1I        173  CAGCTG               C[AC]G^C[GT]G
MspI          165  CCGG                 C^CGG
MvaI           43  CCTGG                CC^[AT]GG
PvuII         173  CAGCTG               CAG^CTG
RsaI          448  GTAC                 GT^AC
ScrFI          43  CCTGG                CC^[ACGT]GG
SfcI          352  CTACAG               C^T[GA][CT]AG
TaqI            8  TCGA                 T^CGA
Tru9I         119  TTAA                 T^TAA
TseI          172  GCAGC                G^C[AT]GC
TspRI         191  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I         8  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcsI          208  GAATTT               [GA]^AATT[CT]
              316  AAATTT              
ApoI          208  GAATTT               [GA]^AATT[CT]
              316  AAATTT              
BanII          31  GGGCCC               G[GA]GC[CT]^C
              404  GAGCCC              
BmyI           31  GGGCCC               G[AGT]GC[ACT]^C
              404  GAGCCC              
BsiYI          43  CCTGGACACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              409  CCTCTTGCAGG         
BslI           43  CCTGGACACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              409  CCTCTTGCAGG         
Bsp1286I       31  GGGCCC               G[AGT]GC[ACT]^C
              404  GAGCCC              
BstF5I        390  CATCC                GGATG(2/0)
              465  GGATG               
CfoI          129  GCGC                 GCG^C
              306  GCGC                
EarI          279  GAAGAG               CTCTTC(1/4)
              436  GAAGAG              
FokI          390  CATCC                GGATG(9/13)
              465  GGATG               
HhaI          129  GCGC                 GCG^C
              306  GCGC                
HinP1I        129  GCGC                 G^CGC
              306  GCGC                
Ksp632I       279  GAAGAG               CTCTTC(1/4)
              436  GAAGAG              
NlaIII         54  CATG                 CATG^
              474  CATG                
NlaIV          30  GGGGCC               GG[ACGT]^[ACGT]CC
               31  GGGCCC              
SfaNI         271  GATGC                GCATC(5/9)
              481  GATGC               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

AluI        Cfr13I      MboII       MnlI        MwoI        
Sau96I      Tsp509I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com