Restriction map for pF1KB8278
##################################
# NO CUT ENZYMES (  179 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaI        AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BglI        BglII       BlnI        
BlpI        BmyI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiHKAI     BsiWI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrFI       
BsrGI       BssHII      BssSI       BstAPI      BstBI       
BstUI       BstYI       Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoRV       EcoT14I     FauI        FbaI        
FseI        FspI        HaeII       HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HpaI        HpaII       KasI        KpnI        KspI        
MamI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MslI        MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   36 enzymes) #
###################################

AciI          902  GCGG                 CCGC(-3/-1)
AcsI          406  GAATTC               [GA]^AATT[CT]
AflII         522  CTTAAG               C^TTAAG
AflIII        121  ACGTGT               A^C[GA][CT]GT
AlwI          429  GGATC                GGATC(4/5)
ApoI          406  GAATTC               [GA]^AATT[CT]
AvaII         655  GGTCC                G^G[AT]CC
BfaI          818  CTAG                 C^TAG
BfrI          522  CTTAAG               C^TTAAG
BsaI          142  GAGACC               GGTCTC(1/5)
BsaJI         250  CCAGGG               C^C[ACGT][ACGT]GG
BsgI          835  GTGCAG               GTGCAG(16/14)
BsrDI         505  GCAATG               GCAATG(2/0)
Bst1107I      235  GTATAC               GTA^TAC
BstEII        904  GGTAACC              G^GT[ACGT]ACC
BstPI         904  GGTAACC              G^GT[ACGT]ACC
BstXI         714  CCAGAGAGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I       235  GTATAC               GTA^TAC
Cfr13I        655  GGTCC                G^G[ACGT]CC
DraI          597  TTTAAA               TTT^AAA
EcoNI         641  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       904  GGTAACC              G^GT[ACGT]ACC
EcoRI         406  GAATTC               G^AATTC
EcoT22I         1  ATGCAT               ATGCA^T
HaeIII        771  GGCC                 GG^CC
HindIII       393  AAGCTT               A^AGCTT
MaeI          818  CTAG                 C^TAG
MaeII         121  ACGT                 A^CGT
MspA1I        900  CAGCGG               C[AC]G^C[GT]G
MwoI          310  GCTGATGCTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIII        579  CATG                 CATG^
NsiI            1  ATGCAT               ATGCA^T
PleI          754  GACTC                GAGTC(4/5)
Sau96I        655  GGTCC                G^G[ACGT]CC
SmlI          522  CTTAAG               C^T[CT][GA]AG
TfiI           17  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AccI          101  GTCTAC               GT^[AC][GT]AC
              235  GTATAC              
BsiYI         641  CCTGGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              781  CCAGCAGGAGG         
BslI          641  CCTGGAGAAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              781  CCAGCAGGAGG         
BsmAI         142  GAGAC                GTCTC(1/5)
              840  GTCTC               
BstF5I        301  CATCC                GGATG(2/0)
              847  CATCC               
EarI          804  CTCTTC               CTCTTC(1/4)
              273  GAAGAG              
FokI          301  CATCC                GGATG(9/13)
              847  CATCC               
HinfI          17  GAATC                G^A[ACGT]TC
              754  GACTC               
HphI          471  GGTGA                GGTGA(8/7)
              711  TCACC               
Ksp632I       804  CTCTTC               CTCTTC(1/4)
              273  GAAGAG              
MseI          523  TTAA                 T^TAA
              598  TTAA                
NlaIV         591  GGAACC               GG[ACGT]^[ACGT]CC
              883  GGAACC              
ScaI          826  AGTACT               AGT^ACT
              871  AGTACT              
Tru9I         523  TTAA                 T^TAA
              598  TTAA                
TspRI          27  CACTG                CA[GC]TG[ACGT][ACGT]^
              833  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AfaI        AluI        BbvI        BpmI        BseRI       
BsrI        BssKI       BstNI       DdeI        DpnII       
EcoRII      Fnu4HI      ItaI        MaeIII      MboI        
MboII       MnlI        MvaI        NdeII       RsaI        
Sau3AI      ScrFI       SfaNI       TaqI        TseI        
Tsp45I      Tsp509I     TthHB8I     
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