Restriction map for pF1KB6463
 (Linear) (Six-base) MAPSORT of: KIBB6463.seq  Check: 6439  from: 1  to: 966



KIBB6463.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:47  ..

Acc65I G'GTAC_C

Cuts at:      0     412     966 
   Size:        412     554
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     907     966 
   Size:        580     327      59

  Fragments arranged by size: 

                 580     327      59
 
AleI CACnn'nnGTG

Cuts at:      0     848     966 
   Size:        848     118
 
ApoI r'AATT_y

Cuts at:      0     178     393     431     760     966 
   Size:        178     215      38     329     206

  Fragments arranged by size: 

                 329     215     206     178      38
 
AseI AT'TA_AT

Cuts at:      0     554     966 
   Size:        554     412
 
BamHI G'GATC_C

Cuts at:      0      54     966 
   Size:         54     912
 
BanI G'GyrC_C

Cuts at:      0     412     966 
   Size:        412     554
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     116     344     966 
   Size:        116     228     622

  Fragments arranged by size: 

                 622     228     116
 
BglII A'GATC_T

Cuts at:      0     279     966 
   Size:        279     687
 
Bme1580I G_kGCm'C

Cuts at:      0     618     966 
   Size:        618     348
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      55     411     966 
   Size:         55     356     555

  Fragments arranged by size: 

                 555     356      55
 
BsaHI Gr'CG_yC

Cuts at:      0       4     966 
   Size:          4     962
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     787     966 
   Size:        787     179
 
Bsp1286I G_dGCh'C

Cuts at:      0     618     966 
   Size:        618     348
 
BspHI T'CATG_A

Cuts at:      0     941     966 
   Size:        941      25
 
BsrDI GCAATG_nn'

Cuts at:      0     351     966 
   Size:        351     615
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     525     966 
   Size:        525     441
 
BstYI r'GATC_y

Cuts at:      0      54     279     966 
   Size:         54     225     687

  Fragments arranged by size: 

                 687     225      54
 
Bsu36I CC'TnA_GG

Cuts at:      0     788     966 
   Size:        788     178
 
BtgI C'CryG_G

Cuts at:      0      26     966 
   Size:         26     940
 
BtsI GCAGTG_nn'

Cuts at:      0     263     966 
   Size:        263     703
 
DraI TTT'AAA

Cuts at:      0     392     506     966 
   Size:        392     114     460

  Fragments arranged by size: 

                 460     392     114
 
EarI CTCTTCn'nnn_

Cuts at:      0      74     137     810     966 
   Size:         74      63     673     156

  Fragments arranged by size: 

                 673     156      74      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     580     907     966 
   Size:        580     327      59

  Fragments arranged by size: 

                 580     327      59
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     666     966 
   Size:        666     300
 
HindIII A'AGCT_T

Cuts at:      0     185     966 
   Size:        185     781
 
KpnI G_GTAC'C

Cuts at:      0     416     966 
   Size:        416     550
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     930     966 
   Size:        930      36
 
MslI CAynn'nnrTG

Cuts at:      0     848     966 
   Size:        848     118
 
MspA1I CmG'CkG

Cuts at:      0     492     966 
   Size:        492     474
 
PstI C_TGCA'G

Cuts at:      0     236     270     966 
   Size:        236      34     696

  Fragments arranged by size: 

                 696     236      34
 
PvuII CAG'CTG

Cuts at:      0     492     966 
   Size:        492     474
 
SfcI C'TryA_G

Cuts at:      0     232     266     966 
   Size:        232      34     700

  Fragments arranged by size: 

                 700     232      34
 
SspI AAT'ATT

Cuts at:      0     174     966 
   Size:        174     792
 
Tth111I GACn'n_nGTC

Cuts at:      0     151     966 
   Size:        151     815
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     522     966 
   Size:        522     444
 
XmnI GAAnn'nnTTC

Cuts at:      0     431     830     966 
   Size:        431     399     136

  Fragments arranged by size: 

                 431     399     136
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     ApoI     AseI    BamHI     BanI     BbsI
    BglII Bme1580I     BmrI    BsaHI    BseRI Bsp1286I    BspHI    BsrDI
    BstXI    BstYI   Bsu36I     BtgI     BtsI     DraI     EarI  Eco57MI
     FalI  HindIII     KpnI     MmeI     MslI   MspA1I     PstI    PvuII
     SfcI     SspI  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI
     AvaI    AvrII     BaeI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
  BstZ17I    BtgZI     ClaI    CspCI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI
    TaqII     TatI     TstI     XbaI     XhoI     XmaI     ZraI
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