Restriction map for
pF1KE0558
(Linear) (Six-base) MAPSORT of: KIEE0558.seq Check: 314 from: 1 to: 1203
KIEE0558.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1186 1203
Size: 1186 17
AatII G_ACGT'C
Cuts at: 0 728 983 1203
Size: 728 255 220
Fragments arranged by size:
728 255 220
AccI GT'mk_AC
Cuts at: 0 23 401 1203
Size: 23 378 802
Fragments arranged by size:
802 378 23
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 304 336 772 804 1203
Size: 304 32 436 32 399
Fragments arranged by size:
436 399 304 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 102 309 573 576 1203
Size: 102 207 264 3 627
Fragments arranged by size:
627 264 207 102 3
ApaI G_GGCC'C
Cuts at: 0 236 861 1203
Size: 236 625 342
Fragments arranged by size:
625 342 236
ApaLI G'TGCA_C
Cuts at: 0 1117 1162 1203
Size: 1117 45 41
Fragments arranged by size:
1117 45 41
ApoI r'AATT_y
Cuts at: 0 754 1203
Size: 754 449
AvaI C'yCGr_G
Cuts at: 0 852 1203
Size: 852 351
BanI G'GyrC_C
Cuts at: 0 505 932 1203
Size: 505 427 271
Fragments arranged by size:
505 427 271
BanII G_rGCy'C
Cuts at: 0 236 861 965 1203
Size: 236 625 104 238
Fragments arranged by size:
625 238 236 104
BbeI G_GCGC'C
Cuts at: 0 509 936 1203
Size: 509 427 267
Fragments arranged by size:
509 427 267
BbsI GAAGACnn'nnnn_
Cuts at: 0 593 720 1203
Size: 593 127 483
Fragments arranged by size:
593 483 127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 796 830 1203
Size: 796 34 373
Fragments arranged by size:
796 373 34
BglI GCCn_nnn'nGGC
Cuts at: 0 599 1203
Size: 599 604
BlpI GC'TnA_GC
Cuts at: 0 267 1203
Size: 267 936
Bme1580I G_kGCm'C
Cuts at: 0 236 535 861 1121 1166 1203
Size: 236 299 326 260 45 37
Fragments arranged by size:
326 299 260 236 45 37
BmgBI CAC'GTC
Cuts at: 0 249 696 1203
Size: 249 447 507
Fragments arranged by size:
507 447 249
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 331 979 1021 1177 1203
Size: 148 183 648 42 156 26
Fragments arranged by size:
648 183 156 148 42 26
Bpu10I CC'TnA_GC
Cuts at: 0 1136 1203
Size: 1136 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 652 1203
Size: 634 18 551
Fragments arranged by size:
634 551 18
BsaHI Gr'CG_yC
Cuts at: 0 506 725 933 980 1203
Size: 506 219 208 47 223
Fragments arranged by size:
506 223 219 208 47
BsaWI w'CCGG_w
Cuts at: 0 26 1203
Size: 26 1177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 304 334 1203
Size: 304 30 869
Fragments arranged by size:
869 304 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 1203
Size: 565 638
BseYI C'CCAG_C
Cuts at: 0 528 1008 1203
Size: 528 480 195
Fragments arranged by size:
528 480 195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 408 922 1036 1203
Size: 408 514 114 167
Fragments arranged by size:
514 408 167 114
BsiEI CG_ry'CG
Cuts at: 0 444 974 1203
Size: 444 530 229
Fragments arranged by size:
530 444 229
BsiHKAI G_wGCw'C
Cuts at: 0 965 1121 1166 1202 1203
Size: 965 156 45 36 1
Fragments arranged by size:
965 156 45 36 1
Bsp1286I G_dGCh'C
Cuts at: 0 236 535 861 965 1121 1166 1202 1203
Size: 236 299 326 104 156 45 36 1
Fragments arranged by size:
326 299 236 156 104 45 36 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 413 755 1186 1203
Size: 413 342 431 17
Fragments arranged by size:
431 413 342 17
BsrBI CCG'CTC
Cuts at: 0 480 1203
Size: 480 723
BsrFI r'CCGG_y
Cuts at: 0 767 916 1203
Size: 767 149 287
Fragments arranged by size:
767 287 149
BssSI C'ACGA_G
Cuts at: 0 55 1030 1111 1203
Size: 55 975 81 92
Fragments arranged by size:
975 92 81 55
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1125 1203
Size: 1125 78
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 1113 1203
Size: 219 894 90
Fragments arranged by size:
894 219 90
BstYI r'GATC_y
Cuts at: 0 656 1203
Size: 656 547
BtgI C'CryG_G
Cuts at: 0 218 488 1022 1203
Size: 218 270 534 181
Fragments arranged by size:
534 270 218 181
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 410 812 1203
Size: 410 402 391
Fragments arranged by size:
410 402 391
BtsI GCAGTG_nn'
Cuts at: 0 345 619 1203
Size: 345 274 584
Fragments arranged by size:
584 345 274
DrdI GACnn_nn'nnGTC
Cuts at: 0 724 730 1203
Size: 724 6 473
Fragments arranged by size:
724 473 6
EaeI y'GGCC_r
Cuts at: 0 59 914 1203
Size: 59 855 289
Fragments arranged by size:
855 289 59
EarI CTCTTCn'nnn_
Cuts at: 0 340 526 1203
Size: 340 186 677
Fragments arranged by size:
677 340 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 104 448 872 1203
Size: 104 344 424 331
Fragments arranged by size:
424 344 331 104
EcoICRI GAG'CTC
Cuts at: 0 963 1203
Size: 963 240
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 331 979 1021 1177 1203
Size: 148 183 648 42 156 26
Fragments arranged by size:
648 183 156 148 42 26
EcoO109I rG'GnC_Cy
Cuts at: 0 232 233 306 857 858 1203
Size: 232 1 73 551 1 345
Fragments arranged by size:
551 345 232 73 1 1
FspI TGC'GCA
Cuts at: 0 514 1203
Size: 514 689
FspAI rTGC'GCAy
Cuts at: 0 514 1203
Size: 514 689
HaeII r_GCGC'y
Cuts at: 0 509 936 1203
Size: 509 427 267
Fragments arranged by size:
509 427 267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 469 501 1203
Size: 469 32 702
Fragments arranged by size:
702 469 32
HincII GTy'rAC
Cuts at: 0 735 1203
Size: 735 468
KasI G'GCGC_C
Cuts at: 0 505 932 1203
Size: 505 427 271
Fragments arranged by size:
505 427 271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 99 1203
Size: 99 1104
MslI CAynn'nnrTG
Cuts at: 0 217 1111 1203
Size: 217 894 92
Fragments arranged by size:
894 217 92
MspA1I CmG'CkG
Cuts at: 0 162 490 573 1203
Size: 162 328 83 630
Fragments arranged by size:
630 328 162 83
NaeI GCC'GGC
Cuts at: 0 918 1203
Size: 918 285
NarI GG'CG_CC
Cuts at: 0 506 933 1203
Size: 506 427 270
Fragments arranged by size:
506 427 270
NcoI C'CATG_G
Cuts at: 0 218 1203
Size: 218 985
NgoMIV G'CCGG_C
Cuts at: 0 916 1203
Size: 916 287
NspI r_CATG'y
Cuts at: 0 996 1203
Size: 996 207
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 1203
Size: 309 894
PfoI T'CCnGG_A
Cuts at: 0 308 1057 1203
Size: 308 749 146
Fragments arranged by size:
749 308 146
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 336 1203
Size: 304 32 867
Fragments arranged by size:
867 304 32
PpuMI rG'GwC_Cy
Cuts at: 0 306 1203
Size: 306 897
PspOMI G'GGCC_C
Cuts at: 0 232 857 1203
Size: 232 625 346
Fragments arranged by size:
625 346 232
PstI C_TGCA'G
Cuts at: 0 107 614 809 1203
Size: 107 507 195 394
Fragments arranged by size:
507 394 195 107
PvuI CG_AT'CG
Cuts at: 0 974 1203
Size: 974 229
PvuII CAG'CTG
Cuts at: 0 162 573 1203
Size: 162 411 630
Fragments arranged by size:
630 411 162
SacI G_AGCT'C
Cuts at: 0 965 1203
Size: 965 238
SacII CC_GC'GG
Cuts at: 0 491 1203
Size: 491 712
SfcI C'TryA_G
Cuts at: 0 103 610 805 1203
Size: 103 507 195 398
Fragments arranged by size:
507 398 195 103
SfoI GGC'GCC
Cuts at: 0 507 934 1203
Size: 507 427 269
Fragments arranged by size:
507 427 269
SmaI CCC'GGG
Cuts at: 0 854 1203
Size: 854 349
SmlI C'TyrA_G
Cuts at: 0 649 667 1203
Size: 649 18 536
Fragments arranged by size:
649 536 18
StuI AGG'CCT
Cuts at: 0 241 1203
Size: 241 962
StyI C'CwwG_G
Cuts at: 0 218 1203
Size: 218 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 135 414 1203
Size: 135 279 789
Fragments arranged by size:
789 279 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 149 181 1203
Size: 149 32 1022
Fragments arranged by size:
1022 149 32
Tth111I GACn'n_nGTC
Cuts at: 0 979 1203
Size: 979 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 151 1155 1203
Size: 151 1004 48
Fragments arranged by size:
1004 151 48
XmaI C'CCGG_G
Cuts at: 0 852 1203
Size: 852 351
ZraI GAC'GTC
Cuts at: 0 726 981 1203
Size: 726 255 222
Fragments arranged by size:
726 255 222
Enzymes that do cut:
AarI AatII AccI AloI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BcgI BglI BlpI
Bme1580I BmgBI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FspI
FspAI HaeII Hin4I HincII KasI MmeI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PfoI PpiI
PpuMI PspOMI PstI PvuI PvuII SacI SacII SfcI
SfoI SmaI SmlI StuI StyI TaqII TstI Tth111I
XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BclI BfrBI BglII BmrI BmtI BplI
BsaI BsaAI BsaBI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DraIII EagI EcoNI EcoRI EcoRV FalI
FseI HindIII HpaI KpnI MfeI MluI MscI NdeI
NheI NotI NruI NsiI PacI PasI PciI PmeI
PmlI PshAI PsiI PspXI PsrI RsrII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI XbaI XhoI
XmnI