Restriction map for
pF1KB6890
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BseAI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MseI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 154 GAATTT [GA]^AATT[CT]
AcyI 229 GACGCC G[GA]^CG[CT]C
AfaI 348 GTAC GT^AC
AflIII 209 ACATGT A^C[GA][CT]GT
AlwI 165 GATCC GGATC(4/5)
AlwNI 254 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 186 GGGCCC GGGCC^C
ApoI 154 GAATTT [GA]^AATT[CT]
BalI 2 TGGCCA TGG^CCA
BclI 159 TGATCA T^GATCA
BfaI 169 CTAG C^TAG
BsaHI 229 GACGCC G[GA]^CG[CT]C
BsaI 490 GAGACC GGTCTC(1/5)
BsaWI 65 TCCGGT [AT]^CCGG[AT]
BseRI 577 CTCCTC GAGGAG(10/8)
BsmFI 458 GGGAC GGGAC(10/14)
BspLU11I 209 ACATGT A^CATGT
BspMI 292 ACCTGC ACCTGC(4/8)
BsrI 455 ACTGG ACTGG(1/-1)
BstEII 435 GGTGACC G^GT[ACGT]ACC
BstPI 435 GGTGACC G^GT[ACGT]ACC
BstYI 164 AGATCC [GA]^GATC[CT]
Cac8I 195 GCCAGC GC[ACGT]^[ACGT]GC
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoO65I 435 GGTGACC G^GT[ACGT]ACC
FbaI 159 TGATCA T^GATCA
HgaI 229 GACGC GACGC(5/10)
Hin1I 229 GACGCC G[GA]^CG[CT]C
HincII 337 GTCAAC GT[CT]^[GA]AC
HindII 337 GTCAAC GT[CT]^[GA]AC
HinfI 172 GACTC G^A[ACGT]TC
MaeI 169 CTAG C^TAG
MboII 120 GAAGA GAAGA(8/7)
MflI 164 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 318 CATCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 406 CAGCGG C[AC]G^C[GT]G
NspI 209 ACATGT [GA]CATG^[CT]
PleI 172 GACTC GAGTC(4/5)
PpuMI 497 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 276 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsaI 348 GTAC GT^AC
SmaI 431 CCCGGG CCC^GGG
Tsp509I 155 AATT ^AATT
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 164 AGATCC [GA]^GATC[CT]
XmaCI 431 CCCGGG C^CCGGG
XmaI 431 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaI 42 CCCGAG C^[CT]CG[GA]G
431 CCCGGG
BcnI 431 CCCGG CC^[GC]GG
432 CCGGG
BsoBI 42 CCCGAG C^[CT]CG[GA]G
431 CCCGGG
DraII 186 GGGCCCT [GA]G^G[ACGT]CC[CT]
497 AGGACCC
EcoO109I 186 GGGCCCT [GA]G^G[ACGT]CC[CT]
497 AGGACCC
FauI 375 CCCGC CCCGC(4/6)
440 CCCGC
HapII 66 CCGG C^CGG
432 CCGG
HpaII 66 CCGG C^CGG
432 CCGG
HphI 435 GGTGA GGTGA(8/7)
290 TCACC
MaeII 23 ACGT A^CGT
335 ACGT
MaeIII 274 GTGAC ^GT[ACGT]AC
436 GTGAC
MspI 66 CCGG C^CGG
432 CCGG
NciI 431 CCCGG CC^[GC]GG
432 CCGGG
NcoI 303 CCATGG C^CATGG
593 CCATGG
Tsp45I 274 GTGAC ^GT[GC]AC
436 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII DsaI
EcoRII EcoT14I Fnu4HI FokI HaeIII
ItaI MboI MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TseI
TthHB8I