Restriction map for pF1KB6382
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BbvI        BcgI        
BclI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaBI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstNI       BstPI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoO65I     EcoRI       EcoRII      EcoRV       
EcoT22I     FbaI        FseI        FspI        HaeII       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MunI        MvaI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NruI        NsiI        
NspV        PacI        PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
TseI        Van91I      XbaI        XcmI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          184  AAATTC               [GA]^AATT[CT]
AcyI          637  GACGCC               G[GA]^CG[CT]C
AfaI          237  GTAC                 GT^AC
ApoI          184  AAATTC               [GA]^AATT[CT]
Asp700I       298  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          475  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          707  CTCGAG               C^[CT]CG[GA]G
BciVI         268  GGATAC               GTATCC(6/5)
BcnI          741  CCGGG                CC^[GC]GG
BsaAI         791  CACGTA               [CT]AC^GT[GA]
BsaHI         637  GACGCC               G[GA]^CG[CT]C
BsaI          448  GAGACC               GGTCTC(1/5)
BsaJI         314  CCAAGG               C^C[ACGT][ACGT]GG
BsaWI          81  ACCGGA               [AT]^CCGG[AT]
BsgI          751  CTGCAC               GTGCAG(16/14)
BsiEI          65  CGGCCG               CG[GA][CT]^CG
BsmBI          37  GAGACG               CGTCTC(1/5)
BsmFI         743  GGGAC                GGGAC(10/14)
BsoBI         707  CTCGAG               C^[CT]CG[GA]G
BspMI         749  ACCTGC               ACCTGC(4/8)
BsrI          701  CCAGT                ACTGG(1/-1)
BssKI         741  CCGGG                ^CC[ACGT]GG
BstAPI        205  GCACATGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DdeI          773  CTAAG                C^T[ACGT]AG
DraIII        206  CACATGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          517  GACGATGTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI           65  CGGCCG               [CT]^GGCC[GA]
EagI           65  CGGCCG               C^GGCCG
EarI          397  GAAGAG               CTCTTC(1/4)
EclXI          65  CGGCCG               C^GGCCG
Eco52I         65  CGGCCG               C^GGCCG
EcoNI           6  CCTGCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       314  CCAAGG               C^C[AT][AT]GG
Hin1I         637  GACGCC               G[GA]^CG[CT]C
Ksp632I       397  GAAGAG               CTCTTC(1/4)
MspA1I        641  CCGCTG               C[AC]G^C[GT]G
NciI          741  CCGGG                CC^[GC]GG
NotI           64  GCGGCCGC             GC^GGCCGC
NspI          341  GCATGT               [GA]CATG^[CT]
PaeR7I        707  CTCGAG               C^TCGAG
PflFI         475  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RsaI          237  GTAC                 GT^AC
ScrFI         741  CCGGG                CC^[ACGT]GG
StuI          776  AGGCCT               AGG^CCT
StyI          314  CCAAGG               C^C[AT][AT]GG
Tth111I       475  GACAGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          707  CTCGAG               C^TCGAG
XmnI          298  GAAAATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AflIII        734  ACACGT               A^C[GA][CT]GT
              790  ACACGT              
AluI          336  AGCT                 AG^CT
              623  AGCT                
AlwI            3  GATCC                GGATC(4/5)
              729  GATCC               
AspHI         704  GTGCTC               G[AT]GC[AT]^C
              768  GTGCTC              
AvaII         120  GGTCC                G^G[AT]CC
              591  GGTCC               
BbsI          882  GAAGAC               GAAGAC(2/6)
              490  GTCTTC              
BmyI          704  GTGCTC               G[AGT]GC[ACT]^C
              768  GTGCTC              
BpmI           10  CTGGAG               CTGGAG(16/14)
              619  CTGGAG              
BpuAI         882  GAAGAC               GAAGAC(2/6)
              490  GTCTTC              
BseRI         614  CTCCTC               GAGGAG(10/8)
               34  GAGGAG              
BsiHKAI       704  GTGCTC               G[AT]GC[AT]^C
              768  GTGCTC              
Bsp1286I      704  GTGCTC               G[AGT]GC[ACT]^C
              768  GTGCTC              
CfoI           46  GCGC                 GCG^C
              836  GCGC                
Cfr13I        120  GGTCC                G^G[ACGT]CC
              591  GGTCC               
DpnII           3  GATC                 ^GATC
              729  GATC                
DraII         119  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              590  AGGTCCT             
EcoO109I      119  GGGTCCT              [GA]G^G[ACGT]CC[CT]
              590  AGGTCCT             
FauI          896  CCCGC                CCCGC(4/6)
              798  GCGGG               
HaeIII         66  GGCC                 GG^CC
              777  GGCC                
HhaI           46  GCGC                 GCG^C
              836  GCGC                
HinP1I         46  GCGC                 G^CGC
              836  GCGC                
HphI           15  GGTGA                GGTGA(8/7)
              649  GGTGA               
MboI            3  GATC                 ^GATC
              729  GATC                
MseI          226  TTAA                 T^TAA
              506  TTAA                
MslI          691  CACACCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              789  CACACGTATG          
MwoI          205  GCACATGGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              566  GCAGAGCCAGC         
NdeII           3  GATC                 ^GATC
              729  GATC                
NlaIV         119  GGGTCC               GG[ACGT]^[ACGT]CC
              609  GGAACC              
PleI          463  GAGTC                GAGTC(4/5)
              479  GAGTC               
PpuMI         119  GGGTCCT              [GA]G^G[AT]CC[CT]
              590  AGGTCCT             
Sau3AI          3  GATC                 ^GATC
              729  GATC                
Sau96I        120  GGTCC                G^G[ACGT]CC
              591  GGTCC               
TfiI          262  GAATC                G^A[AT]TC
              319  GATTC               
Tru9I         226  TTAA                 T^TAA
              506  TTAA                
TspRI         500  CAGTG                CA[GC]TG[ACGT][ACGT]^
              702  CAGTG               


#########################################
# 3 OR MORE CUT ENZYMES (   28 enzymes) #
#########################################

AccII       AciI        BsiYI       BslI        BsmAI       
BstF5I      BstUI       Cac8I       Fnu4HI      FokI        
HapII       HgaI        HinfI       HpaII       ItaI        
MaeII       MaeIII      MboII       MnlI        MspI        
MvnI        NlaIII      SfaNI       SmlI        TaqI        
Tsp45I      Tsp509I     TthHB8I     
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