Restriction map for pF1KA0006
##################################
# NO CUT ENZYMES (  123 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflIII      
AgeI        AhdI        Alw44I      Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        AspEI       
AviII       AvrII       BalI        BbeI        BbrPI       
BcgI        BcnI        BglI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaHI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrFI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Cac8I       CelII       Cfr10I      ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoNI       EcoO65I     
EcoRI       EcoRV       EcoT22I     FauI        FseI        
FspI        HaeII       HgaI        Hin1I       HindIII     
HpaI        KasI        KspI        MfeI        MluI        
MluNI       MscI        MunI        MvnI        NaeI        
NarI        NciI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StuI        SwaI        XbaI        XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

Acc65I       1406  GGTACC               G^GTACC
AccIII         78  TCCGGA               T^CCGGA
AflII         801  CTTAAG               C^TTAAG
AlwNI        1288  CAGATACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         2071  CCTGAGG              CC^T[ACGT]AGG
Asp718I      1406  GGTACC               G^GTACC
AspI         2167  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1727  CCCGAG               C^[CT]CG[GA]G
BamHI         174  GGATCC               G^GATCC
BanI         1406  GGTACC               G^G[CT][GA]CC
BanII        1086  GAGCCC               G[GA]GC[CT]^C
BbsI         1899  GAAGAC               GAAGAC(2/6)
BbvCI        1116  CCTCAGC              CCTCAGC(-5/-2)
BfrI          801  CTTAAG               C^TTAAG
BglII        1257  AGATCT               A^GATCT
BpuAI        1899  GAAGAC               GAAGAC(2/6)
BsaBI        1186  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          663  GAGACC               GGTCTC(1/5)
BseAI          78  TCCGGA               T^CCGGA
BsmI         1228  GCATTC               GAATGC(1/-1)
BsoBI        1727  CCCGAG               C^[CT]CG[GA]G
BspEI          78  TCCGGA               T^CCGGA
BsrBI        1402  GAGCGG               CCGCTC(-3/-3)
BssSI         569  CACGAG               CACGAG(-5/-1)
Bsu36I       2071  CCTGAGG              CC^T[ACGT]AGG
CfoI         2006  GCGC                 GCG^C
EaeI         2112  CGGCCA               [CT]^GGCC[GA]
Eco81I       2071  CCTGAGG              CC^T[ACGT]AGG
HhaI         2006  GCGC                 GCG^C
HinP1I       2006  GCGC                 G^CGC
HincII        316  GTCAAC               GT[CT]^[GA]AC
HindII        316  GTCAAC               GT[CT]^[GA]AC
HphI         1506  GGTGA                GGTGA(8/7)
KpnI         1406  GGTACC               GGTAC^C
MamI         1186  GATACTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           78  TCCGGA               T^CCGGA
MslI         1042  CACAGTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI         2025  CCATGG               C^CATGG
NdeI         1177  CATATG               CA^TATG
NspI         1384  GCATGT               [GA]CATG^[CT]
PflFI        2167  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         900  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI          811  CTGCAG               CTGCA^G
SfcI          811  CTGCAG               C^T[GA][CT]AG
SspI          249  AATATT               AAT^ATT
Tth111I      2167  GACGAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        900  CCAAGCCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          956  GTCTAC               GT^[AC][GT]AC
             1778  GTCTAC              
AciI         1404  GCGG                 CCGC(-3/-1)
             2259  GCGG                
AcsI         1882  AAATTT               [GA]^AATT[CT]
             2010  AAATTT              
ApoI         1882  AAATTT               [GA]^AATT[CT]
             2010  AAATTT              
Asp700I      1300  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2042  GAAAAGATTC          
AspHI        1030  GTGCTC               G[AT]GC[AT]^C
             1694  GTGCTC              
BciVI        1185  GGATAC               GTATCC(6/5)
             1094  GTATCC              
BclI          131  TGATCA               T^GATCA
             1637  TGATCA              
BpmI         1138  CTGGAG               CTGGAG(16/14)
             1279  CTGGAG              
BsaWI          78  TCCGGA               [AT]^CCGG[AT]
             1717  ACCGGA              
BsiHKAI      1030  GTGCTC               G[AT]GC[AT]^C
             1694  GTGCTC              
BspMI        1647  ACCTGC               ACCTGC(4/8)
             1785  ACCTGC              
BsrDI        1248  GCAATG               GCAATG(2/0)
             2208  GCAATG              
BstYI         174  GGATCC               [GA]^GATC[CT]
             1257  AGATCT              
DraI           90  TTTAAA               TTT^AAA
              979  TTTAAA              
DsaI         1762  CCGTGG               C^C[GA][CT]GG
             2025  CCATGG              
FbaI          131  TGATCA               T^GATCA
             1637  TGATCA              
HaeIII       2023  GGCC                 GG^CC
             2113  GGCC                
HapII          79  CCGG                 C^CGG
             1718  CCGG                
HpaII          79  CCGG                 C^CGG
             1718  CCGG                
MaeII         360  ACGT                 A^CGT
              563  ACGT                
MflI          174  GGATCC               [GA]^GATC[CT]
             1257  AGATCT              
MspI           79  CCGG                 C^CGG
             1718  CCGG                
SmlI          801  CTTAAG               C^T[CT][GA]AG
             2031  CTCAAG              
XhoII         174  GGATCC               [GA]^GATC[CT]
             1257  AGATCT              
XmnI         1300  GAACCTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2042  GAAAAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   62 enzymes) #
#########################################

AfaI        AluI        AlwI        AvaII       BbvI        
BfaI        BmyI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HinfI       
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScaI        ScrFI       SfaNI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
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