Restriction map for pF1KA0006
 (Linear) (Six-base) MAPSORT of: KIAA0006.seq  Check: 9487  from: 1  to: 2328



KIAA0006.orf

 With 182 enzymes: SgfI * 

                        October 24, 2019 20:49  ..

AccI GT'mk_AC

Cuts at:      0     957    1779    2328 
   Size:        957     822     549

  Fragments arranged by size: 

                 957     822     549
 
Acc65I G'GTAC_C

Cuts at:      0    1406    2328 
   Size:       1406     922
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     763    1001    2203    2328 
   Size:        206     557     238    1202     125

  Fragments arranged by size: 

                1202     557     238     206     125
 
AflII C'TTAA_G

Cuts at:      0     801    2328 
   Size:        801    1527
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1293    2328 
   Size:       1293    1035
 
ApoI r'AATT_y

Cuts at:      0    1882    2010    2328 
   Size:       1882     128     318

  Fragments arranged by size: 

                1882     318     128
 
AvaI C'yCGr_G

Cuts at:      0    1727    2328 
   Size:       1727     601
 
BamHI G'GATC_C

Cuts at:      0     174    2328 
   Size:        174    2154
 
BanI G'GyrC_C

Cuts at:      0    1406    2328 
   Size:       1406     922
 
BanII G_rGCy'C

Cuts at:      0    1090    2328 
   Size:       1090    1238
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1906    2328 
   Size:       1906     422
 
BbvCI CC'TCA_GC

Cuts at:      0    1117    2328 
   Size:       1117    1211
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1105    1179    2328 
   Size:       1105      74    1149

  Fragments arranged by size: 

                1149    1105      74
 
BclI T'GATC_A

Cuts at:      0     131    1637    2328 
   Size:        131    1506     691

  Fragments arranged by size: 

                1506     691     131
 
BglII A'GATC_T

Cuts at:      0    1257    2328 
   Size:       1257    1071
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     623     865    2328 
   Size:        623     242    1463

  Fragments arranged by size: 

                1463     623     242
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1159    1300    2328 
   Size:       1159     141    1028

  Fragments arranged by size: 

                1159    1028     141
 
Bpu10I CC'TnA_GC

Cuts at:      0    1117    2328 
   Size:       1117    1211
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2016    2328 
   Size:       2016     312
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     657    2328 
   Size:        657    1671
 
BsaBI GATnn'nnATC

Cuts at:      0    1190    2328 
   Size:       1190    1138
 
BsaWI w'CCGG_w

Cuts at:      0      78    1717    2328 
   Size:         78    1639     611

  Fragments arranged by size: 

                1639     611      78
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1758    1788    2328 
   Size:       1758      30     540

  Fragments arranged by size: 

                1758     540      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      97    1414    1736    1936    2328 
   Size:         97    1317     322     200     392

  Fragments arranged by size: 

                1317     392     322     200      97
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1393    1524    1772    1983    2328 
   Size:       1393     131     248     211     345

  Fragments arranged by size: 

                1393     345     248     211     131
 
BsiHKAI G_wGCw'C

Cuts at:      0    1034    1698    2328 
   Size:       1034     664     630

  Fragments arranged by size: 

                1034     664     630
 
BsmI GAATG_Cn'

Cuts at:      0    1228    2328 
   Size:       1228    1100
 
Bsp1286I G_dGCh'C

Cuts at:      0    1034    1090    1698    2328 
   Size:       1034      56     608     630

  Fragments arranged by size: 

                1034     630     608      56
 
BspEI T'CCGG_A

Cuts at:      0      78    2328 
   Size:         78    2250
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1656    1794    2328 
   Size:       1656     138     534

  Fragments arranged by size: 

                1656     534     138
 
BsrBI CCG'CTC

Cuts at:      0    1404    2328 
   Size:       1404     924
 
BsrDI GCAATG_nn'

Cuts at:      0    1255    2215    2328 
   Size:       1255     960     113

  Fragments arranged by size: 

                1255     960     113
 
BssSI C'ACGA_G

Cuts at:      0     569    2328 
   Size:        569    1759
 
BstYI r'GATC_y

Cuts at:      0     174    1257    2328 
   Size:        174    1083    1071

  Fragments arranged by size: 

                1083    1071     174
 
Bsu36I CC'TnA_GG

Cuts at:      0    2072    2328 
   Size:       2072     256
 
BtgI C'CryG_G

Cuts at:      0    1762    2025    2328 
   Size:       1762     263     303

  Fragments arranged by size: 

                1762     303     263
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     437     472    2328 
   Size:        437      35    1856

  Fragments arranged by size: 

                1856     437      35
 
DraI TTT'AAA

Cuts at:      0      92     981    2328 
   Size:         92     889    1347

  Fragments arranged by size: 

                1347     889      92
 
EaeI y'GGCC_r

Cuts at:      0    2112    2328 
   Size:       2112     216
 
EarI CTCTTCn'nnn_

Cuts at:      0     372     791    1261    1955    2072    2328 
   Size:        372     419     470     694     117     256

  Fragments arranged by size: 

                 694     470     419     372     256     117
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     763    1001    1159    1300    2203    2328 
   Size:        206     557     238     158     141     903     125

  Fragments arranged by size: 

                 903     557     238     206     158     141     125
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1645    1732    2241    2328 
   Size:       1645      87     509      87

  Fragments arranged by size: 

                1645     509      87      87
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     325     357     520     552     832     864    1819    1851 
   Size:        325      32     163      32     280      32     955      32

Cuts at:   1851    2328 
   Size:        477

  Fragments arranged by size: 

                 955     477     325     280     163      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0     318    2328 
   Size:        318    2010
 
KpnI G_GTAC'C

Cuts at:      0    1410    2328 
   Size:       1410     918
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1034    1598    2328 
   Size:       1034     564     730

  Fragments arranged by size: 

                1034     730     564
 
MslI CAynn'nnrTG

Cuts at:      0    1046    2328 
   Size:       1046    1282
 
MspA1I CmG'CkG

Cuts at:      0    1021    1278    1626    1951    2328 
   Size:       1021     257     348     325     377

  Fragments arranged by size: 

                1021     377     348     325     257
 
NcoI C'CATG_G

Cuts at:      0    2025    2328 
   Size:       2025     303
 
NdeI CA'TA_TG

Cuts at:      0    1178    2328 
   Size:       1178    1150
 
NspI r_CATG'y

Cuts at:      0    1388    2328 
   Size:       1388     940
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     906    2328 
   Size:        906    1422
 
PfoI T'CCnGG_A

Cuts at:      0     746    1607    2328 
   Size:        746     861     721

  Fragments arranged by size: 

                 861     746     721
 
PpuMI rG'GwC_Cy

Cuts at:      0    1645    1732    2241    2328 
   Size:       1645      87     509      87

  Fragments arranged by size: 

                1645     509      87      87
 
PsiI TTA'TAA

Cuts at:      0    1756    1823    2328 
   Size:       1756      67     505

  Fragments arranged by size: 

                1756     505      67
 
PstI C_TGCA'G

Cuts at:      0     815    2328 
   Size:        815    1513
 
PvuII CAG'CTG

Cuts at:      0    1021    1278    1626    1951    2328 
   Size:       1021     257     348     325     377

  Fragments arranged by size: 

                1021     377     348     325     257
 
ScaI AGT'ACT

Cuts at:      0     303     834    2037    2328 
   Size:        303     531    1203     291

  Fragments arranged by size: 

                1203     531     303     291
 
SfcI C'TryA_G

Cuts at:      0     811    2328 
   Size:        811    1517
 
SmlI C'TyrA_G

Cuts at:      0     801    2031    2328 
   Size:        801    1230     297

  Fragments arranged by size: 

                1230     801     297
 
SspI AAT'ATT

Cuts at:      0     251    2328 
   Size:        251    2077
 
StyI C'CwwG_G

Cuts at:      0     290     905    1736    2025    2328 
   Size:        290     615     831     289     303

  Fragments arranged by size: 

                 831     615     303     290     289
 
TatI w'GTAC_w

Cuts at:      0     301     832    1945    2035    2328 
   Size:        301     531    1113      90     293

  Fragments arranged by size: 

                1113     531     301     293      90
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1490    1522    2328 
   Size:       1490      32     806

  Fragments arranged by size: 

                1490     806      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    2170    2328 
   Size:       2170     158
 
XmnI GAAnn'nnTTC

Cuts at:      0    1304    2046    2328 
   Size:       1304     742     282

  Fragments arranged by size: 

                1304     742     282
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI    AflII    AlwNI     ApoI     AvaI    BamHI
     BanI    BanII     BbsI    BbvCI    BciVI     BclI    BglII     BmrI
     BpmI   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI
     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI
    BssSI    BstYI   Bsu36I     BtgI    CspCI     DraI     EaeI     EarI
  Eco57MI EcoO109I    Hin4I   HincII     KpnI     MmeI     MslI   MspA1I
     NcoI     NdeI     NspI    PflMI     PfoI    PpuMI     PsiI     PstI
    PvuII     ScaI     SfcI     SmlI     SspI     StyI     TatI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI     BbeI     BcgI    BfrBI     BglI     BlpI Bme1580I    BmgBI
     BmtI     BplI    BsaAI    BsaHI    BseYI    BsiEI    BsiWI    BsmBI
    BspHI    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI
  BstZ17I    BtgZI     BtsI     ClaI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     SwaI    TaqII     XbaI     XcmI     XhoI
     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com