Restriction map for pF1KE0348
 (Linear) (Six-base) MAPSORT of: KIEE0348.seq  Check: 5270  from: 1  to: 1509



KIEE0348.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     246     838     846     971    1509 
   Size:        246     592       8     125     538

  Fragments arranged by size: 

                 592     538     246     125       8
 
AccI GT'mk_AC

Cuts at:      0     911    1394    1509 
   Size:        911     483     115

  Fragments arranged by size: 

                 911     483     115
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     322     556    1509 
   Size:        322     234     953

  Fragments arranged by size: 

                 953     322     234
 
AfeI AGC'GCT

Cuts at:      0     886    1509 
   Size:        886     623
 
AlwNI CAG_nnn'CTG

Cuts at:      0     279     300     468     543     612     783    1509 
   Size:        279      21     168      75      69     171     726

  Fragments arranged by size: 

                 726     279     171     168      75      69      21
 
ApaI G_GGCC'C

Cuts at:      0    1459    1509 
   Size:       1459      50
 
ApaLI G'TGCA_C

Cuts at:      0    1099    1509 
   Size:       1099     410
 
ApoI r'AATT_y

Cuts at:      0    1020    1509 
   Size:       1020     489
 
AvaI C'yCGr_G

Cuts at:      0     592    1509 
   Size:        592     917
 
BanI G'GyrC_C

Cuts at:      0     403     797    1012    1253    1509 
   Size:        403     394     215     241     256

  Fragments arranged by size: 

                 403     394     256     241     215
 
BanII G_rGCy'C

Cuts at:      0     161    1459    1509 
   Size:        161    1298      50

  Fragments arranged by size: 

                1298     161      50
 
BbeI G_GCGC'C

Cuts at:      0    1257    1509 
   Size:       1257     252
 
BbvCI CC'TCA_GC

Cuts at:      0     649    1509 
   Size:        649     860
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1153    1187    1509 
   Size:       1153      34     322

  Fragments arranged by size: 

                1153     322      34
 
BlpI GC'TnA_GC

Cuts at:      0     461     679    1245    1509 
   Size:        461     218     566     264

  Fragments arranged by size: 

                 566     461     264     218
 
Bme1580I G_kGCm'C

Cuts at:      0     406    1103    1335    1459    1509 
   Size:        406     697     232     124      50

  Fragments arranged by size: 

                 697     406     232     124      50
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1161    1170    1509 
   Size:       1161       9     339

  Fragments arranged by size: 

                1161     339       9
 
BmtI G_CTAG'C

Cuts at:      0      16    1509 
   Size:         16    1493
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     187     289     367     547     550     634     726 
   Size:        168      19     102      78     180       3      84      92

Cuts at:    726    1367    1509 
   Size:        641     142

  Fragments arranged by size: 

                 641     180     168     142     102      92      84      78
                  19       3
 
Bpu10I CC'TnA_GC

Cuts at:      0     649    1509 
   Size:        649     860
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     354    1509 
   Size:        354    1155
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     186     499     610    1055    1509 
   Size:        186     313     111     445     454

  Fragments arranged by size: 

                 454     445     313     186     111
 
BsaHI Gr'CG_yC

Cuts at:      0    1254    1364    1391    1509 
   Size:       1254     110      27     118

  Fragments arranged by size: 

                1254     118     110      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      25     646    1509 
   Size:         25     621     863

  Fragments arranged by size: 

                 863     621      25
 
BseYI C'CCAG_C

Cuts at:      0       8     251     399    1065    1069    1083    1459    1509 
   Size:          8     243     148     666       4      14     376      50

  Fragments arranged by size: 

                 666     376     243     148      50      14       8       4
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     214     893    1509 
   Size:        214     679     616

  Fragments arranged by size: 

                 679     616     214
 
BsiHKAI G_wGCw'C

Cuts at:      0    1079    1103    1509 
   Size:       1079      24     406

  Fragments arranged by size: 

                1079     406      24
 
Bsp1286I G_dGCh'C

Cuts at:      0     161     406    1079    1103    1335    1459    1509 
   Size:        161     245     673      24     232     124      50

  Fragments arranged by size: 

                 673     245     232     161     124      50      24
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     246     342     838     846     971    1509 
   Size:        246      96     496       8     125     538

  Fragments arranged by size: 

                 538     496     246     125      96       8
 
BsrDI GCAATG_nn'

Cuts at:      0    1095    1509 
   Size:       1095     414
 
BssHII G'CGCG_C

Cuts at:      0     587    1509 
   Size:        587     922
 
BssSI C'ACGA_G

Cuts at:      0    1162    1509 
   Size:       1162     347
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     279     771     804    1509 
   Size:        279     492      33     705

  Fragments arranged by size: 

                 705     492     279      33
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      60     528     889    1509 
   Size:         60     468     361     620

  Fragments arranged by size: 

                 620     468     361      60
 
BstYI r'GATC_y

Cuts at:      0     434     878    1509 
   Size:        434     444     631

  Fragments arranged by size: 

                 631     444     434
 
Bsu36I CC'TnA_GG

Cuts at:      0     176    1509 
   Size:        176    1333
 
BtsI GCAGTG_nn'

Cuts at:      0     226     277     301     425     978    1206    1468    1509 
   Size:        226      51      24     124     553     228     262      41

  Fragments arranged by size: 

                 553     262     228     226     124      51      41      24
            
 
DraIII CAC_nnn'GTG

Cuts at:      0     296     804    1474    1509 
   Size:        296     508     670      35

  Fragments arranged by size: 

                 670     508     296      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     513    1509 
   Size:        513     996
 
EaeI y'GGCC_r

Cuts at:      0     120     218     638    1509 
   Size:        120      98     420     871

  Fragments arranged by size: 

                 871     420     120      98
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     100    1509 
   Size:        100    1409
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     168     187     289     322     367     547     550     556 
   Size:        168      19     102      33      45     180       3       6

Cuts at:    556     634     726    1367    1509 
   Size:         78      92     641     142

  Fragments arranged by size: 

                 641     180     168     142     102      92      78      45
                  33      19       6       3
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     751    1509 
   Size:        751     758
 
EcoO109I rG'GnC_Cy

Cuts at:      0     172     609    1455    1456    1509 
   Size:        172     437     846       1      53

  Fragments arranged by size: 

                 846     437     172      53       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     348    1509 
   Size:        348    1161
 
HaeII r_GCGC'y

Cuts at:      0     695     888    1002    1257    1509 
   Size:        695     193     114     255     252

  Fragments arranged by size: 

                 695     255     252     193     114
 
KasI G'GCGC_C

Cuts at:      0    1253    1509 
   Size:       1253     256
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     218    1509 
   Size:        218    1291
 
MscI TGG'CCA

Cuts at:      0     220     640    1509 
   Size:        220     420     869

  Fragments arranged by size: 

                 869     420     220
 
MslI CAynn'nnrTG

Cuts at:      0      58     922    1509 
   Size:         58     864     587

  Fragments arranged by size: 

                 864     587      58
 
MspA1I CmG'CkG

Cuts at:      0     267     540     780    1029    1069    1241    1439    1509 
   Size:        267     273     240     249      40     172     198      70

  Fragments arranged by size: 

                 273     267     249     240     198     172      70      40
            
 
NarI GG'CG_CC

Cuts at:      0    1254    1509 
   Size:       1254     255
 
NheI G'CTAG_C

Cuts at:      0      12    1509 
   Size:         12    1497
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     783    1509 
   Size:        783     726
 
PfoI T'CCnGG_A

Cuts at:      0     863    1509 
   Size:        863     646
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     844     876    1509 
   Size:        844      32     633

  Fragments arranged by size: 

                 844     633      32
 
PshAI GACnn'nnGTC

Cuts at:      0    1316    1509 
   Size:       1316     193
 
PspOMI G'GGCC_C

Cuts at:      0    1455    1509 
   Size:       1455      54
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1198    1230    1509 
   Size:       1198      32     279

  Fragments arranged by size: 

                1198     279      32
 
PstI C_TGCA'G

Cuts at:      0      26     284     755     968    1277    1509 
   Size:         26     258     471     213     309     232

  Fragments arranged by size: 

                 471     309     258     232     213      26
 
PvuII CAG'CTG

Cuts at:      0     267     540     780    1069    1509 
   Size:        267     273     240     289     440

  Fragments arranged by size: 

                 440     289     273     267     240
 
SbfI CC_TGCA'GG

Cuts at:      0     755    1509 
   Size:        755     754
 
SexAI A'CCwGG_T

Cuts at:      0     800    1509 
   Size:        800     709
 
SfcI C'TryA_G

Cuts at:      0      22     126     280     547     751     964    1273    1509 
   Size:         22     104     154     267     204     213     309     236

  Fragments arranged by size: 

                 309     267     236     213     204     154     104      22
            
 
SfoI GGC'GCC

Cuts at:      0    1255    1509 
   Size:       1255     254
 
SmlI C'TyrA_G

Cuts at:      0     333     592    1509 
   Size:        333     259     917

  Fragments arranged by size: 

                 917     333     259
 
StuI AGG'CCT

Cuts at:      0    1168    1509 
   Size:       1168     341
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     265     463    1509 
   Size:        265     198    1046

  Fragments arranged by size: 

                1046     265     198
 
TatI w'GTAC_w

Cuts at:      0    1418    1509 
   Size:       1418      91
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      95     127    1509 
   Size:         95      32    1382

  Fragments arranged by size: 

                1382      95      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     396     525    1342    1509 
   Size:         57     339     129     817     167

  Fragments arranged by size: 

                 817     339     167     129      57
 
XhoI C'TCGA_G

Cuts at:      0     592    1509 
   Size:        592     917
 
XmnI GAAnn'nnTTC

Cuts at:      0     952    1509 
   Size:        952     557
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AfeI    AlwNI     ApaI    ApaLI     ApoI
     AvaI     BanI    BanII     BbeI    BbvCI     BcgI     BlpI Bme1580I
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BsrDI   BssHII    BssSI
   BstAPI    BstXI    BstYI   Bsu36I     BtsI   DraIII     DrdI     EaeI
     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII     KasI     MmeI
     MscI     MslI   MspA1I     NarI     NheI    PflMI     PfoI     PpiI
    PshAI   PspOMI     PsrI     PstI    PvuII     SbfI    SexAI     SfcI
     SfoI     SmlI     StuI    TaqII     TatI     TstI     XcmI     XhoI
     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbsI    BciVI     BclI    BfrBI     BglI    BglII    BmgBI     BplI
    BsaAI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BsrBI    BsrFI    BsrGI    BstBI   BstEII  BstZ17I
     BtgI    BtgZI     ClaI    CspCI     DraI     EagI     EarI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     NaeI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI    PpuMI     PsiI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI     SfiI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI  Tth111I
     XbaI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com