Restriction map for pF1KE0327
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaLI       AscI        Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbeI        BbvCI       BclI        BfrI        BlnI        
BlpI        Bpu1102I    BsaHI       BsaI        BseAI       
BsiEI       BsiWI       BsmBI       BspEI       BspLU11I    
BsrBI       BsrFI       BssHII      BssSI       BstBI       
BstEII      BstPI       BstXI       Bsu36I      CelII       
Cfr10I      DraI        DrdI        DsaI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoT22I     FbaI        FseI        
FspI        HgaI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MluI        MroI        NaeI        NarI        NcoI        
NgoMIV      NotI        NruI        NsiI        NspV        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        SacI        SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmlI        SpeI        
SphI        Sse8387I    StuI        SwaI        Tth111I     
Van91I      XhoI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

AccI         1782  GTATAC               GT^[AC][GT]AC
AccII         114  CGCG                 CG^CG
AlwNI          22  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         1333  GGGCCC               GGGCC^C
AseI          922  ATTAAT               AT^TAAT
AsnI          922  ATTAAT               AT^TAAT
AvaI         1658  CCCGGG               C^[CT]CG[GA]G
BbrPI         502  CACGTG               CAC^GTG
BcgI          501  GCACGTGAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI           26  GCCTGAAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1732  AGATCT               A^GATCT
BpmI         1228  CTGGAG               CTGGAG(16/14)
BsaBI        1342  GATTTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         122  ACCGGA               [AT]^CCGG[AT]
BsmAI        1139  GTCTC                GTCTC(1/5)
BsoBI        1658  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      590  TGTACA               T^GTACA
BspDI        1439  ATCGAT               AT^CGAT
BspHI         728  TCATGA               T^CATGA
BspMI         998  ACCTGC               ACCTGC(4/8)
BsrGI         590  TGTACA               T^GTACA
Bst1107I     1782  GTATAC               GTA^TAC
BstUI         114  CGCG                 CG^CG
BstZ17I      1782  GTATAC               GTA^TAC
ClaI         1439  ATCGAT               AT^CGAT
CpoI          124  CGGACCG              CG^G[AT]CCG
DraIII       1204  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EcoRI         386  GAATTC               G^AATTC
EcoT14I      1049  CCTTGG               C^C[AT][AT]GG
HphI          831  GGTGA                GGTGA(8/7)
MamI         1342  GATTTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         1470  CAATTG               C^AATTG
MunI         1470  CAATTG               C^AATTG
MvnI          114  CGCG                 CG^CG
NdeI          323  CATATG               CA^TATG
NheI          519  GCTAGC               G^CTAGC
NspI          545  GCATGT               [GA]CATG^[CT]
PacI          919  TTAATTAA             TTAAT^TAA
PleI          507  GAGTC                GAGTC(4/5)
PmaCI         502  CACGTG               CAC^GTG
PmlI          502  CACGTG               CAC^GTG
PpuMI        1533  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI         922  ATTAAT               AT^TAAT
PstI         1003  CTGCAG               CTGCA^G
RcaI          728  TCATGA               T^CATGA
RsrII         124  CGGACCG              CG^G[AT]CCG
SapI         1585  GAAGAGC              GCTCTTC(1/4)
SmaI         1658  CCCGGG               CCC^GGG
SnaBI        1792  TACGTA               TAC^GTA
SspBI         590  TGTACA               T^GTACA
StyI         1049  CCTTGG               C^C[AT][AT]GG
Tsp45I       1243  GTGAC                ^GT[GC]AC
XmaCI        1658  CCCGGG               C^CCGGG
XmaI         1658  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AlwI          172  GGATC                GGATC(4/5)
             1694  GGATC               
Asp700I       292  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              744  GAAGAGTTTC          
AspHI         118  GAGCAC               G[AT]GC[AT]^C
             1231  GAGCAC              
BanI          162  GGCACC               G^G[CT][GA]CC
              789  GGTGCC              
BbsI          431  GTCTTC               GAAGAC(2/6)
             1273  GTCTTC              
BpuAI         431  GTCTTC               GAAGAC(2/6)
             1273  GTCTTC              
BsaAI         502  CACGTG               [CT]AC^GT[GA]
             1792  TACGTA              
BseRI         872  CTCCTC               GAGGAG(10/8)
             1102  GAGGAG              
BsgI         1163  CTGCAC               GTGCAG(16/14)
              689  GTGCAG              
BsiHKAI       118  GAGCAC               G[AT]GC[AT]^C
             1231  GAGCAC              
BsmFI        1393  GTCCC                GGGAC(10/14)
             1762  GTCCC               
BsmI          565  GCATTC               GAATGC(1/-1)
             1753  GCATTC              
BsrDI         639  CATTGC               GCAATG(2/0)
              708  CATTGC              
BstAPI        373  GCATTTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              545  GCATGTTTTGC         
BstYI         172  GGATCT               [GA]^GATC[CT]
             1732  AGATCT              
DraII        1333  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1533  AGGACCT             
EarI          744  GAAGAG               CTCTTC(1/4)
             1585  GAAGAG              
EcoO109I     1333  GGGCCCC              [GA]G^G[ACGT]CC[CT]
             1533  AGGACCT             
HaeII          23  AGCGCC               [GA]GCGC^[CT]
             1147  GGCGCT              
Ksp632I       744  GAAGAG               CTCTTC(1/4)
             1585  GAAGAG              
MflI          172  GGATCT               [GA]^GATC[CT]
             1732  AGATCT              
MseI          919  TTAA                 T^TAA
              923  TTAA                
PvuII         368  CAGCTG               CAG^CTG
              659  CAGCTG              
SfaNI         516  GATGC                GCATC(5/9)
             1723  GATGC               
SspI          311  AATATT               AAT^ATT
             1670  AATATT              
TfiI          405  GATTC                G^A[AT]TC
             1578  GAATC               
Tru9I         919  TTAA                 T^TAA
              923  TTAA                
XbaI         1581  TCTAGA               T^CTAGA
             1729  TCTAGA              
XcmI          638  CCATTGCTGTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1057  CCAATGTATATTTGG     
XhoII         172  GGATCT               [GA]^GATC[CT]
             1732  AGATCT              
XmnI          292  GAAGGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              744  GAAGAGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
AvaII       BalI        BanII       BbvI        BciVI       
BcnI        BfaI        BmyI        BsaJI       BsiYI       
BslI        Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoRV       FauI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfcI        TaqI        TseI        Tsp509I     TspRI       
TthHB8I     
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