Restriction map for pF1KE0327
 (Linear) (Six-base) MAPSORT of: KIEE0327.seq  Check: 2910  from: 1  to: 1854



KIEE0327.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

AccI GT'mk_AC

Cuts at:      0    1783    1854 
   Size:       1783      71
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     188    1153    1465    1854 
   Size:         49     139     965     312     389

  Fragments arranged by size: 

                 965     389     312     139      49
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27    1854 
   Size:         27    1827
 
ApaI G_GGCC'C

Cuts at:      0    1337    1854 
   Size:       1337     517
 
ApoI r'AATT_y

Cuts at:      0     386     936    1023    1354    1854 
   Size:        386     550      87     331     500

  Fragments arranged by size: 

                 550     500     386     331      87
 
AseI AT'TA_AT

Cuts at:      0     923    1854 
   Size:        923     931
 
AvaI C'yCGr_G

Cuts at:      0    1658    1854 
   Size:       1658     196
 
BanI G'GyrC_C

Cuts at:      0     162     789    1854 
   Size:        162     627    1065

  Fragments arranged by size: 

                1065     627     162
 
BanII G_rGCy'C

Cuts at:      0      18    1289    1337    1854 
   Size:         18    1271      48     517

  Fragments arranged by size: 

                1271     517      48      18
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     424    1266    1854 
   Size:        424     842     588

  Fragments arranged by size: 

                 842     588     424
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     490     524    1854 
   Size:        490      34    1330

  Fragments arranged by size: 

                1330     490      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      48    1136    1358    1854 
   Size:         48    1088     222     496

  Fragments arranged by size: 

                1088     496     222      48
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32    1854 
   Size:         32    1822
 
BglII A'GATC_T

Cuts at:      0    1732    1854 
   Size:       1732     122
 
Bme1580I G_kGCm'C

Cuts at:      0     165    1337    1854 
   Size:        165    1172     517

  Fragments arranged by size: 

                1172     517     165
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     270    1854 
   Size:        270    1584
 
BmtI G_CTAG'C

Cuts at:      0     523    1854 
   Size:        523    1331
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1249    1854 
   Size:       1249     605
 
Bpu10I CC'TnA_GC

Cuts at:      0     223    1854 
   Size:        223    1631
 
BsaAI yAC'GTr

Cuts at:      0     504    1794    1854 
   Size:        504    1290      60

  Fragments arranged by size: 

                1290     504      60
 
BsaBI GATnn'nnATC

Cuts at:      0    1346    1854 
   Size:       1346     508
 
BsaWI w'CCGG_w

Cuts at:      0     122    1854 
   Size:        122    1732
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1038    1068    1854 
   Size:       1038      30     786

  Fragments arranged by size: 

                1038     786      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     863    1117    1854 
   Size:        863     254     737

  Fragments arranged by size: 

                 863     737     254
 
BseYI C'CCAG_C

Cuts at:      0      90     318     648     661    1447    1854 
   Size:         90     228     330      13     786     407

  Fragments arranged by size: 

                 786     407     330     228      90      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1148    1854 
   Size:        710     438     706

  Fragments arranged by size: 

                 710     706     438
 
BsiHKAI G_wGCw'C

Cuts at:      0     122    1235    1854 
   Size:        122    1113     619

  Fragments arranged by size: 

                1113     619     122
 
BsmI GAATG_Cn'

Cuts at:      0     565    1753    1854 
   Size:        565    1188     101

  Fragments arranged by size: 

                1188     565     101
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     122     165    1235    1289    1337    1854 
   Size:         18     104      43    1070      54      48     517

  Fragments arranged by size: 

                1070     517     104      54      48      43      18
 
BspHI T'CATG_A

Cuts at:      0     728    1854 
   Size:        728    1126
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1007    1854 
   Size:       1007     847
 
BsrDI GCAATG_nn'

Cuts at:      0     638     707    1854 
   Size:        638      69    1147

  Fragments arranged by size: 

                1147     638      69
 
BsrGI T'GTAC_A

Cuts at:      0     590    1854 
   Size:        590    1264
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     379     551    1854 
   Size:        379     172    1303

  Fragments arranged by size: 

                1303     379     172
 
BstYI r'GATC_y

Cuts at:      0     172    1732    1854 
   Size:        172    1560     122

  Fragments arranged by size: 

                1560     172     122
 
BstZ17I GTA'TAC

Cuts at:      0    1784    1854 
   Size:       1784      70
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     441    1854 
   Size:        441    1413
 
BtsI GCAGTG_nn'

Cuts at:      0      54    1854 
   Size:         54    1800
 
ClaI AT'CG_AT

Cuts at:      0    1440    1854 
   Size:       1440     414
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1021    1056    1533    1568    1854 
   Size:       1021      35     477      35     286

  Fragments arranged by size: 

                1021     477     286      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1209    1854 
   Size:       1209     645
 
EaeI y'GGCC_r

Cuts at:      0     364     679    1541    1854 
   Size:        364     315     862     313

  Fragments arranged by size: 

                 862     364     315     313
 
EarI CTCTTCn'nnn_

Cuts at:      0     739    1580    1854 
   Size:        739     841     274

  Fragments arranged by size: 

                 841     739     274
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     188    1153    1249    1465    1854 
   Size:         49     139     965      96     216     389

  Fragments arranged by size: 

                 965     389     216     139      96      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1334    1534    1854 
   Size:       1334     200     320

  Fragments arranged by size: 

                1334     320     200
 
EcoRI G'AATT_C

Cuts at:      0     386    1854 
   Size:        386    1468
 
EcoRV GAT'ATC

Cuts at:      0    1222    1327    1608    1854 
   Size:       1222     105     281     246

  Fragments arranged by size: 

                1222     281     246     105
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     768    1854 
   Size:        768    1086
 
HaeII r_GCGC'y

Cuts at:      0      27    1151    1854 
   Size:         27    1124     703

  Fragments arranged by size: 

                1124     703      27
 
MfeI C'AATT_G

Cuts at:      0    1470    1854 
   Size:       1470     384
 
MscI TGG'CCA

Cuts at:      0     366     681    1543    1854 
   Size:        366     315     862     311

  Fragments arranged by size: 

                 862     366     315     311
 
MslI CAynn'nnrTG

Cuts at:      0     544     643    1134    1854 
   Size:        544      99     491     720

  Fragments arranged by size: 

                 720     544     491      99
 
MspA1I CmG'CkG

Cuts at:      0      38     370     661    1854 
   Size:         38     332     291    1193

  Fragments arranged by size: 

                1193     332     291      38
 
NdeI CA'TA_TG

Cuts at:      0     324    1854 
   Size:        324    1530
 
NheI G'CTAG_C

Cuts at:      0     519    1854 
   Size:        519    1335
 
NspI r_CATG'y

Cuts at:      0     549    1854 
   Size:        549    1305
 
PacI TTA_AT'TAA

Cuts at:      0     923    1854 
   Size:        923     931
 
PmlI CAC'GTG

Cuts at:      0     504    1854 
   Size:        504    1350
 
PpuMI rG'GwC_Cy

Cuts at:      0    1534    1854 
   Size:       1534     320
 
PsiI TTA'TAA

Cuts at:      0    1075    1854 
   Size:       1075     779
 
PspOMI G'GGCC_C

Cuts at:      0    1333    1854 
   Size:       1333     521
 
PstI C_TGCA'G

Cuts at:      0    1007    1854 
   Size:       1007     847
 
PvuII CAG'CTG

Cuts at:      0     370     661    1854 
   Size:        370     291    1193

  Fragments arranged by size: 

                1193     370     291
 
RsrII CG'GwC_CG

Cuts at:      0     125    1854 
   Size:        125    1729
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1580    1854 
   Size:       1580     274
 
SfcI C'TryA_G

Cuts at:      0    1003    1370    1707    1854 
   Size:       1003     367     337     147

  Fragments arranged by size: 

                1003     367     337     147
 
SmaI CCC'GGG

Cuts at:      0    1660    1854 
   Size:       1660     194
 
SnaBI TAC'GTA

Cuts at:      0    1794    1854 
   Size:       1794      60
 
SspI AAT'ATT

Cuts at:      0     313    1672    1854 
   Size:        313    1359     182

  Fragments arranged by size: 

                1359     313     182
 
StyI C'CwwG_G

Cuts at:      0    1049    1854 
   Size:       1049     805
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     142    1854 
   Size:        142    1712
 
TatI w'GTAC_w

Cuts at:      0     590    1184    1854 
   Size:        590     594     670

  Fragments arranged by size: 

                 670     594     590
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1830    1854 
   Size:       1830      24
 
XbaI T'CTAG_A

Cuts at:      0    1581    1729    1854 
   Size:       1581     148     125

  Fragments arranged by size: 

                1581     148     125
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     645    1064    1854 
   Size:        645     419     790

  Fragments arranged by size: 

                 790     645     419
 
XmaI C'CCGG_G

Cuts at:      0    1658    1854 
   Size:       1658     196
 
XmnI GAAnn'nnTTC

Cuts at:      0     296     748    1854 
   Size:        296     452    1106

  Fragments arranged by size: 

                1106     452     296
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     ApaI     ApoI     AseI     AvaI     BanI
    BanII     BbsI     BcgI    BciVI     BglI    BglII Bme1580I     BmrI
     BmtI     BpmI   Bpu10I    BsaAI    BsaBI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrDI
    BsrGI   BstAPI    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI
   DraIII     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI
    HaeII     MfeI     MscI     MslI   MspA1I     NdeI     NheI     NspI
     PacI     PmlI    PpuMI     PsiI   PspOMI     PstI    PvuII    RsrII
     SapI     SfcI     SmaI    SnaBI     SspI     StyI    TaqII     TatI
     TstI     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI    AsiSI    AvrII
     BaeI    BamHI     BbeI    BbvCI     BclI    BfrBI     BlpI    BmgBI
     BplI    BpuEI     BsaI    BsaHI    BsiEI    BsiWI    BsmBI    BspEI
    BsrBI    BsrFI   BssHII    BssSI    BstBI   BstEII    BstXI   Bsu36I
     BtgI     DraI     DrdI     EagI     EciI  EcoICRI    EcoNI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MluI     MmeI     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NsiI     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PshAI
    PspXI     PsrI     PvuI     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmlI     SpeI
     SphI     SrfI     StuI     SwaI  Tth111I     XhoI     ZraI
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