Restriction map for pF1KE0436
 (Linear) (Six-base) MAPSORT of: KIEE0436.seq  Check: 7897  from: 1  to: 1092



KIEE0436.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AccI GT'mk_AC

Cuts at:      0     170    1092 
   Size:        170     922
 
AclI AA'CG_TT

Cuts at:      0     849    1092 
   Size:        849     243
 
AfeI AGC'GCT

Cuts at:      0     146    1092 
   Size:        146     946
 
AflII C'TTAA_G

Cuts at:      0     981    1092 
   Size:        981     111
 
AflIII A'CryG_T

Cuts at:      0      23      83     339     603    1092 
   Size:         23      60     256     264     489

  Fragments arranged by size: 

                 489     264     256      60      23
 
AleI CACnn'nnGTG

Cuts at:      0      82    1092 
   Size:         82    1010
 
ApoI r'AATT_y

Cuts at:      0     436     745     770     880    1092 
   Size:        436     309      25     110     212

  Fragments arranged by size: 

                 436     309     212     110      25
 
BanI G'GyrC_C

Cuts at:      0      72    1092 
   Size:         72    1020
 
BbeI G_GCGC'C

Cuts at:      0      76    1092 
   Size:         76    1016
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     964    1092 
   Size:        964     128
 
BlpI GC'TnA_GC

Cuts at:      0     401    1092 
   Size:        401     691
 
Bme1580I G_kGCm'C

Cuts at:      0      70    1092 
   Size:         70    1022
 
BsaAI yAC'GTr

Cuts at:      0      26      84     351     477    1092 
   Size:         26      58     267     126     615

  Fragments arranged by size: 

                 615     267     126      58      26
 
BsaHI Gr'CG_yC

Cuts at:      0      73    1092 
   Size:         73    1019
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      13      43    1092 
   Size:         13      30    1049

  Fragments arranged by size: 

                1049      30      13
 
BseYI C'CCAG_C

Cuts at:      0      49    1092 
   Size:         49    1043
 
BsiEI CG_ry'CG

Cuts at:      0     156    1092 
   Size:        156     936
 
BsiHKAI G_wGCw'C

Cuts at:      0      20      62     188    1092 
   Size:         20      42     126     904

  Fragments arranged by size: 

                 904     126      42      20
 
BsmI GAATG_Cn'

Cuts at:      0     333    1092 
   Size:        333     759
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      62      70     188    1092 
   Size:         20      42       8     118     904

  Fragments arranged by size: 

                 904     118      42      20       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      50    1092 
   Size:         50    1042
 
BsrFI r'CCGG_y

Cuts at:      0     155    1092 
   Size:        155     937
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     574    1092 
   Size:        574     518
 
BstBI TT'CG_AA

Cuts at:      0     854    1092 
   Size:        854     238
 
BstYI r'GATC_y

Cuts at:      0     897    1092 
   Size:        897     195
 
BtsI GCAGTG_nn'

Cuts at:      0     435    1092 
   Size:        435     657
 
EaeI y'GGCC_r

Cuts at:      0     314    1092 
   Size:        314     778
 
EarI CTCTTCn'nnn_

Cuts at:      0     193     717    1092 
   Size:        193     524     375

  Fragments arranged by size: 

                 524     375     193
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     105    1092 
   Size:        105     987
 
HaeII r_GCGC'y

Cuts at:      0      76     148    1092 
   Size:         76      72     944

  Fragments arranged by size: 

                 944      76      72
 
HincII GTy'rAC

Cuts at:      0     291    1092 
   Size:        291     801
 
KasI G'GCGC_C

Cuts at:      0      72    1092 
   Size:         72    1020
 
MscI TGG'CCA

Cuts at:      0     316    1092 
   Size:        316     776
 
MslI CAynn'nnrTG

Cuts at:      0      82    1006    1092 
   Size:         82     924      86

  Fragments arranged by size: 

                 924      86      82
 
NarI GG'CG_CC

Cuts at:      0      73    1092 
   Size:         73    1019
 
NdeI CA'TA_TG

Cuts at:      0     827    1092 
   Size:        827     265
 
NspI r_CATG'y

Cuts at:      0     343     607    1005    1092 
   Size:        343     264     398      87

  Fragments arranged by size: 

                 398     343     264      87
 
PciI A'CATG_T

Cuts at:      0     339     603    1092 
   Size:        339     264     489

  Fragments arranged by size: 

                 489     339     264
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     147     180     385    1092 
   Size:        147      33     205     707

  Fragments arranged by size: 

                 707     205     147      33
 
PstI C_TGCA'G

Cuts at:      0     999    1092 
   Size:        999      93
 
SapI GCTCTTCn'nnn_

Cuts at:      0     193    1092 
   Size:        193     899
 
SfcI C'TryA_G

Cuts at:      0     995    1092 
   Size:        995      97
 
SfoI GGC'GCC

Cuts at:      0      74    1092 
   Size:         74    1018
 
SmlI C'TyrA_G

Cuts at:      0     981    1092 
   Size:        981     111
 
SphI G_CATG'C

Cuts at:      0    1005    1092 
   Size:       1005      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     377    1027    1092 
   Size:        377     650      65

  Fragments arranged by size: 

                 650     377      65
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      13      45    1092 
   Size:         13      32    1047

  Fragments arranged by size: 

                1047      32      13
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      84    1092 
   Size:         84    1008
 

 Enzymes that do cut:

     AccI     AclI     AfeI    AflII   AflIII     AleI     ApoI     BanI
     BbeI     BbsI     BlpI Bme1580I    BsaAI    BsaHI    BsaXI    BseYI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspMI    BsrFI   BstAPI    BstBI
    BstYI     BtsI     EaeI     EarI     EciI    HaeII   HincII     KasI
     MscI     MslI     NarI     NdeI     NspI     PciI    PflMI     PstI
     SapI     SfcI     SfoI     SmlI     SphI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AgeI     AhdI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaBI    BsaWI    BseRI     BsgI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI
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