Restriction map for pF1KE0174
##################################
# NO CUT ENZYMES (  154 enzymes) #
##################################

AccIII      AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanII       BbrPI       BbsI        BcgI        BcnI        
BfrI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiHKAI     
BsiWI       BsmAI       BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EaeI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FseI        FspI        HapII       HgaI        HindIII     
HpaII       Ksp632I     KspI        MamI        MluI        
MluNI       MroI        MscI        MspI        NaeI        
NciI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SexAI       SfaNI       SfiI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII         156  GACGTC               GACGT^C
Acc65I         36  GGTACC               G^GTACC
AccI          146  GTAGAC               GT^[AC][GT]AC
AccII          11  CGCG                 CG^CG
AclI          646  AACGTT               AA^CGTT
AlwI          448  GGATC                GGATC(4/5)
Asp718I        36  GGTACC               G^GTACC
AvaII          76  GGTCC                G^G[AT]CC
BbeI          123  GGCGCC               GGCGC^C
BciVI         110  GGATAC               GTATCC(6/5)
BclI          831  TGATCA               T^GATCA
BglI          126  GCCACGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI         289  GAGGAG               GAGGAG(10/8)
BsiEI         783  CGATCG               CG[GA][CT]^CG
BspMI         774  GCAGGT               ACCTGC(4/8)
BsrI          378  ACTGG                ACTGG(1/-1)
BssHII         10  GCGCGC               G^CGCGC
BstUI          11  CGCG                 CG^CG
BstYI         448  GGATCT               [GA]^GATC[CT]
EcoNI         317  CCTGCATAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          120  GCGGG                CCCGC(4/6)
FbaI          831  TGATCA               T^GATCA
HaeII         123  GGCGCC               [GA]GCGC^[CT]
HincII        913  GTTAAC               GT[CT]^[GA]AC
HindII        913  GTTAAC               GT[CT]^[GA]AC
HinfI         438  GATTC                G^A[ACGT]TC
HpaI          913  GTTAAC               GTT^AAC
HphI          834  TCACC                GGTGA(8/7)
KasI          123  GGCGCC               G^GCGCC
KpnI           36  GGTACC               GGTAC^C
MfeI          485  CAATTG               C^AATTG
MflI          448  GGATCT               [GA]^GATC[CT]
MslI          798  CACAGATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          485  CAATTG               C^AATTG
MvnI           11  CGCG                 CG^CG
NarI          123  GGCGCC               GG^CGCC
NcoI          732  CCATGG               C^CATGG
NspI          611  GCATGC               [GA]CATG^[CT]
Psp1406I      646  AACGTT               AA^CGTT
PstI          533  CTGCAG               CTGCA^G
PvuI          783  CGATCG               CGAT^CG
ScaI          789  AGTACT               AGT^ACT
SfoI          123  GGCGCC               GGC^GCC
SphI          611  GCATGC               GCATG^C
TfiI          438  GATTC                G^A[AT]TC
XhoII         448  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AcyI          123  GGCGCC               G[GA]^CG[CT]C
              156  GACGTC              
BanI           36  GGTACC               G^G[CT][GA]CC
              123  GGCGCC              
BbvCI          90  CCTCAGC              CCTCAGC(-5/-2)
              115  CCTCAGC             
BfaI          307  CTAG                 C^TAG
              452  CTAG                
BsaHI         123  GGCGCC               G[GA]^CG[CT]C
              156  GACGTC              
BsmFI          67  GTCCC                GGGAC(10/14)
               77  GTCCC               
Cfr13I         76  GGTCC                G^G[ACGT]CC
              430  GGGCC               
Hin1I         123  GGCGCC               G[GA]^CG[CT]C
              156  GACGTC              
MaeI          307  CTAG                 C^TAG
              452  CTAG                
MaeII         157  ACGT                 A^CGT
              647  ACGT                
MboII         631  GAAGA                GAAGA(8/7)
              236  TCTTC               
PvuII          20  CAGCTG               CAG^CTG
              530  CAGCTG              
Sau96I         76  GGTCC                G^G[ACGT]CC
              430  GGGCC               
TaqI          177  TCGA                 T^CGA
              786  TCGA                
TthHB8I       177  TCGA                 T^CGA
              786  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AciI        AfaI        AluI        BbvI        BsaJI       
BsiYI       BslI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        DdeI        DpnII       DsaI        
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HhaI        HinP1I      ItaI        MaeIII      MboI        
MnlI        MseI        MspA1I      MvaI        MwoI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
ScrFI       SfcI        StyI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       
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