Restriction map for pF1KE0174
 (Linear) (Six-base) MAPSORT of: KIEE0174.seq  Check: 4040  from: 1  to: 918



KIEE0174.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AatII G_ACGT'C

Cuts at:      0     160     918 
   Size:        160     758
 
AccI GT'mk_AC

Cuts at:      0     147     918 
   Size:        147     771
 
Acc65I G'GTAC_C

Cuts at:      0      36     918 
   Size:         36     882
 
AclI AA'CG_TT

Cuts at:      0     647     918 
   Size:        647     271
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     650     918 
   Size:        650     268
 
AleI CACnn'nnGTG

Cuts at:      0     802     918 
   Size:        802     116
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     530     563     918 
   Size:        530      33     355

  Fragments arranged by size: 

                 530     355      33
 
BanI G'GyrC_C

Cuts at:      0      36     123     918 
   Size:         36      87     795

  Fragments arranged by size: 

                 795      87      36
 
BbeI G_GCGC'C

Cuts at:      0     127     918 
   Size:        127     791
 
BbvCI CC'TCA_GC

Cuts at:      0      91     116     918 
   Size:         91      25     802

  Fragments arranged by size: 

                 802      91      25
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     104     918 
   Size:        104     814
 
BclI T'GATC_A

Cuts at:      0     831     918 
   Size:        831      87
 
BglI GCCn_nnn'nGGC

Cuts at:      0     132     918 
   Size:        132     786
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     388     918 
   Size:        388     530
 
Bpu10I CC'TnA_GC

Cuts at:      0      91     116     918 
   Size:         91      25     802

  Fragments arranged by size: 

                 802      91      25
 
BsaHI Gr'CG_yC

Cuts at:      0     124     157     918 
   Size:        124      33     761

  Fragments arranged by size: 

                 761     124      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     119     149     918 
   Size:        119      30     769

  Fragments arranged by size: 

                 769     119      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     918 
   Size:        304     614
 
BseYI C'CCAG_C

Cuts at:      0      50      56     138     918 
   Size:         50       6      82     780

  Fragments arranged by size: 

                 780      82      50       6
 
BsiEI CG_ry'CG

Cuts at:      0     786     918 
   Size:        786     132
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     765     918 
   Size:        765     153
 
BssHII G'CGCG_C

Cuts at:      0      10     918 
   Size:         10     908
 
BstYI r'GATC_y

Cuts at:      0     448     918 
   Size:        448     470
 
BtgI C'CryG_G

Cuts at:      0      84     127     732     918 
   Size:         84      43     605     186

  Fragments arranged by size: 

                 605     186      84      43
 
BtsI GCAGTG_nn'

Cuts at:      0     681     918 
   Size:        681     237
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     650     918 
   Size:        650     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     321     918 
   Size:        321     597
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     418     918 
   Size:        418     500
 
HaeII r_GCGC'y

Cuts at:      0     127     918 
   Size:        127     791
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     346     378     886     918 
   Size:        346      32     508      32

  Fragments arranged by size: 

                 508     346      32      32
 
HincII GTy'rAC

Cuts at:      0     915     918 
   Size:        915       3
 
HpaI GTT'AAC

Cuts at:      0     915     918 
   Size:        915       3
 
KasI G'GCGC_C

Cuts at:      0     123     918 
   Size:        123     795
 
KpnI G_GTAC'C

Cuts at:      0      40     918 
   Size:         40     878
 
MfeI C'AATT_G

Cuts at:      0     485     918 
   Size:        485     433
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      25     324     426     918 
   Size:         25     299     102     492

  Fragments arranged by size: 

                 492     299     102      25
 
MslI CAynn'nnrTG

Cuts at:      0     802     918 
   Size:        802     116
 
MspA1I CmG'CkG

Cuts at:      0      22     120     532     918 
   Size:         22      98     412     386

  Fragments arranged by size: 

                 412     386      98      22
 
NarI GG'CG_CC

Cuts at:      0     124     918 
   Size:        124     794
 
NcoI C'CATG_G

Cuts at:      0     732     918 
   Size:        732     186
 
NspI r_CATG'y

Cuts at:      0     615     918 
   Size:        615     303
 
PfoI T'CCnGG_A

Cuts at:      0     225     918 
   Size:        225     693
 
PsiI TTA'TAA

Cuts at:      0     203     644     918 
   Size:        203     441     274

  Fragments arranged by size: 

                 441     274     203
 
PstI C_TGCA'G

Cuts at:      0     537     918 
   Size:        537     381
 
PvuI CG_AT'CG

Cuts at:      0     786     918 
   Size:        786     132
 
PvuII CAG'CTG

Cuts at:      0      22     532     918 
   Size:         22     510     386

  Fragments arranged by size: 

                 510     386      22
 
ScaI AGT'ACT

Cuts at:      0     791     918 
   Size:        791     127
 
SfcI C'TryA_G

Cuts at:      0     527     533     908     918 
   Size:        527       6     375      10

  Fragments arranged by size: 

                 527     375      10       6
 
SfoI GGC'GCC

Cuts at:      0     125     918 
   Size:        125     793
 
SphI G_CATG'C

Cuts at:      0     615     918 
   Size:        615     303
 
StyI C'CwwG_G

Cuts at:      0     456     732     757     918 
   Size:        456     276      25     161

  Fragments arranged by size: 

                 456     276     161      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      64     918 
   Size:         64     854
 
TatI w'GTAC_w

Cuts at:      0     653     789     918 
   Size:        653     136     129

  Fragments arranged by size: 

                 653     136     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151     152     184     918 
   Size:        119      32       1      32     734

  Fragments arranged by size: 

                 734     119      32      32       1
 
ZraI GAC'GTC

Cuts at:      0     158     918 
   Size:        158     760
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AleI     BaeI     BanI
     BbeI    BbvCI    BciVI     BclI     BglI     BmrI   Bpu10I    BsaHI
    BsaXI    BseRI    BseYI    BsiEI    BspMI   BssHII    BstYI     BtgI
     BtsI  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MmeI     MslI   MspA1I     NarI     NcoI
     NspI     PfoI     PsiI     PstI     PvuI    PvuII     ScaI     SfcI
     SfoI     SphI     StyI    TaqII     TatI     TstI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI    AflII   AflIII     AgeI     AhdI     AlfI     AloI
    AlwNI     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII    BamHI    BanII     BbsI     BcgI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII     MluI
     MscI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SrfI     SspI     StuI     SwaI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI
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