Restriction map for pF1KB6545
##################################
# NO CUT ENZYMES (  159 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspI        AvaII       AviII       AvrII       
BalI        BanII       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      Cfr13I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
DsaI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO109I    EcoRV       EcoT22I     
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HindIII     HpaI        HpaII       KasI        
KpnI        KspI        MaeI        MamI        MfeI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
Psp1406I    PstI        PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AfaI          492  GTAC                 GT^AC
AspHI         864  GAGCAC               G[AT]GC[AT]^C
AvaI           96  CTCGGG               C^[CT]CG[GA]G
BamHI         964  GGATCC               G^GATCC
BanI          712  GGCACC               G^G[CT][GA]CC
BclI          680  TGATCA               T^GATCA
BlpI          289  GCTCAGC              GC^T[ACGT]AGC
BmyI          864  GAGCAC               G[AGT]GC[ACT]^C
Bpu1102I      289  GCTCAGC              GC^T[ACGT]AGC
BsiHKAI       864  GAGCAC               G[AT]GC[AT]^C
BsmI          642  GAATGC               GAATGC(1/-1)
BsoBI          96  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      864  GAGCAC               G[AGT]GC[ACT]^C
BssSI         832  CACGAG               CACGAG(-5/-1)
BstAPI         56  GCAAAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         289  GCTCAGC              GC^T[ACGT]AGC
CfoI          698  GCGC                 GCG^C
EaeI          667  TGGCCG               [CT]^GGCC[GA]
EcoNI         537  CCTGGACGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         655  GAATTC               G^AATTC
FauI          395  CCCGC                CCCGC(4/6)
FbaI          680  TGATCA               T^GATCA
HhaI          698  GCGC                 GCG^C
HinP1I        698  GCGC                 G^CGC
MspA1I         74  CAGCTG               C[AC]G^C[GT]G
PvuII          74  CAGCTG               CAG^CTG
RsaI          492  GTAC                 GT^AC
SfcI          400  CTACAG               C^T[GA][CT]AG
TaqI         1053  TCGA                 T^CGA
TthHB8I      1053  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AciI          396  CCGC                 CCGC(-3/-1)
             1062  CCGC                
AcsI          655  GAATTC               [GA]^AATT[CT]
              781  GAATTT              
ApoI          655  GAATTC               [GA]^AATT[CT]
              781  GAATTT              
AseI          556  ATTAAT               AT^TAAT
              939  ATTAAT              
AsnI          556  ATTAAT               AT^TAAT
              939  ATTAAT              
BbsI          272  GTCTTC               GAAGAC(2/6)
              894  GTCTTC              
BpuAI         272  GTCTTC               GAAGAC(2/6)
              894  GTCTTC              
BsaI          766  GAGACC               GGTCTC(1/5)
              459  GGTCTC              
BsmAI         766  GAGAC                GTCTC(1/5)
              460  GTCTC               
BsmFI         773  GTCCC                GGGAC(10/14)
              888  GTCCC               
BspMI         577  ACCTGC               ACCTGC(4/8)
              918  ACCTGC              
BstEII         15  GGTCACC              G^GT[ACGT]ACC
              324  GGTCACC             
BstF5I         61  GGATG                GGATG(2/0)
              468  GGATG               
BstPI          15  GGTCACC              G^GT[ACGT]ACC
              324  GGTCACC             
Cac8I         503  GCAAGC               GC[ACGT]^[ACGT]GC
              721  GCCTGC              
EarI          107  CTCTTC               CTCTTC(1/4)
              972  GAAGAG              
EcoO65I        15  GGTCACC              G^GT[ACGT]ACC
              324  GGTCACC             
EcoT14I       708  CCTTGG               C^C[AT][AT]GG
              776  CCAAGG              
FokI           61  GGATG                GGATG(9/13)
              468  GGATG               
HaeIII        307  GGCC                 GG^CC
              668  GGCC                
Ksp632I       107  CTCTTC               CTCTTC(1/4)
              972  GAAGAG              
MaeII         358  ACGT                 A^CGT
              907  ACGT                
MseI          557  TTAA                 T^TAA
              940  TTAA                
NlaIII         82  CATG                 CATG^
              155  CATG                
PleI          270  GAGTC                GAGTC(4/5)
             1026  GAGTC               
PshBI         556  ATTAAT               AT^TAAT
              939  ATTAAT              
SmlI           40  CTCAAG               C^T[CT][GA]AG
              267  CTTGAG              
StyI          708  CCTTGG               C^C[AT][AT]GG
              776  CCAAGG              
Tru9I         557  TTAA                 T^TAA
              940  TTAA                
TspRI         303  CAGTG                CA[GC]TG[ACGT][ACGT]^
              701  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   39 enzymes) #
#########################################

AluI        AlwI        BbvI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsrDI       BsrI        
BssKI       BstNI       BstYI       DdeI        DpnII       
EcoRII      Fnu4HI      HincII      HindII      HinfI       
HphI        ItaI        MaeIII      MboI        MboII       
MflI        MnlI        MvaI        MwoI        NdeII       
NlaIV       Sau3AI      ScrFI       SfaNI       TfiI        
TseI        Tsp45I      Tsp509I     XhoII       
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