Restriction map for pF1KE0965
##################################
# NO CUT ENZYMES (  134 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Alw44I      
AlwNI       AocI        ApaI        ApaLI       AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BclI        BcnI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BspDI       BspEI       BsrBI       BsrFI       
BssHII      BssSI       Bst1107I    BstAPI      BstEII      
BstPI       BstUI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DraIII      EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       FbaI        
FseI        FspI        HapII       HgaI        Hin1I       
HindIII     HpaI        HpaII       KasI        KpnI        
Ksp632I     KspI        MamI        MfeI        MluI        
MroI        MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PstI        PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    StuI        SwaI        Tsp45I      
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AccI          193  GTCTAC               GT^[AC][GT]AC
AflIII         98  ACATGT               A^C[GA][CT]GT
Aor51HI      1136  AGCGCT               AGC^GCT
Asp700I       702  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1089  GAGCAC               G[AT]GC[AT]^C
AvaI          476  CCCGAG               C^[CT]CG[GA]G
BanI         1129  GGCACC               G^G[CT][GA]CC
BanII         505  GGGCTC               G[GA]GC[CT]^C
BbsI          584  GTCTTC               GAAGAC(2/6)
BbvCI         768  GCTGAGG              CCTCAGC(-5/-2)
BciVI         900  GGATAC               GTATCC(6/5)
BglII         704  AGATCT               A^GATCT
BpmI          822  CTCCAG               CTGGAG(16/14)
BpuAI         584  GTCTTC               GAAGAC(2/6)
BsaI          115  GGTCTC               GGTCTC(1/5)
BseRI         711  CTCCTC               GAGGAG(10/8)
BsiHKAI      1089  GAGCAC               G[AT]GC[AT]^C
BsmBI         479  GAGACG               CGTCTC(1/5)
BsoBI         476  CCCGAG               C^[CT]CG[GA]G
BspLU11I       98  ACATGT               A^CATGT
BspMI         650  GCAGGT               ACCTGC(4/8)
BstBI         917  TTCGAA               TT^CGAA
BstYI         704  AGATCT               [GA]^GATC[CT]
DraII         973  GGGACCC              [GA]G^G[ACGT]CC[CT]
DrdI          246  GACCCCGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          269  CCGTGG               C^C[GA][CT]GG
Eco47III     1136  AGCGCT               AGC^GCT
EcoO109I      973  GGGACCC              [GA]G^G[ACGT]CC[CT]
EcoT22I      1078  ATGCAT               ATGCA^T
FauI          249  CCCGC                CCCGC(4/6)
HaeII        1136  AGCGCT               [GA]GCGC^[CT]
HincII         82  GTCAAC               GT[CT]^[GA]AC
HindII         82  GTCAAC               GT[CT]^[GA]AC
MaeIII        296  GTAAC                ^GT[ACGT]AC
MflI          704  AGATCT               [GA]^GATC[CT]
NsiI         1078  ATGCAT               ATGCA^T
NspI           98  ACATGT               [GA]CATG^[CT]
NspV          917  TTCGAA               TT^CGAA
PleI         1454  GACTC                GAGTC(4/5)
PpuMI         973  GGGACCC              [GA]G^G[AT]CC[CT]
SfcI          389  CTGTAG               C^T[GA][CT]AG
SfuI          917  TTCGAA               TT^CGAA
SspI         1021  AATATT               AAT^ATT
XbaI         1158  TCTAGA               T^CTAGA
XhoII         704  AGATCT               [GA]^GATC[CT]
XmnI          702  GAAGATCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

AcsI          354  GAATTT               [GA]^AATT[CT]
              442  AAATTT              
ApoI          354  GAATTT               [GA]^AATT[CT]
              442  AAATTT              
AvaII         135  GGACC                G^G[AT]CC
              974  GGACC               
BsmI         1426  GAATGC               GAATGC(1/-1)
             1080  GCATTC              
Bsp1407I      562  TGTACA               T^GTACA
              752  TGTACA              
BspHI          35  TCATGA               T^CATGA
              855  TCATGA              
BsrGI         562  TGTACA               T^GTACA
              752  TGTACA              
BsrI          290  ACTGG                ACTGG(1/-1)
              824  CCAGT               
BstXI        1169  CCATCCAGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1244  CCAGGCGGATGG        
CfoI          453  GCGC                 GCG^C
             1137  GCGC                
DraI          445  TTTAAA               TTT^AAA
             1008  TTTAAA              
HhaI          453  GCGC                 GCG^C
             1137  GCGC                
HinP1I        453  GCGC                 G^CGC
             1137  GCGC                
MaeII         191  ACGT                 A^CGT
              482  ACGT                
MwoI          149  GCCAGCAGTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1130  GCACCAAGCGC         
PflMI         264  CCAAGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1373  CCAACAGATGG         
RcaI           35  TCATGA               T^CATGA
              855  TCATGA              
SfaNI         765  GATGC                GCATC(5/9)
              173  GCATC               
SspBI         562  TGTACA               T^GTACA
              752  TGTACA              
TfiI           18  GATTC                G^A[AT]TC
             1189  GATTC               
Van91I        264  CCAAGCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1373  CCAACAGATGG         
XcmI          360  CCACTTGGAGGTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1133  CCAAGCGCTACATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BalI        
BbvI        BfaI        BmyI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrDI       
BssKI       BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       EaeI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MboI        MboII       MluNI       
MnlI        MscI        MseI        MslI        MspA1I      
MvaI        NdeII       NlaIII      NlaIV       PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       StyI        
TaqI        Tru9I       TseI        Tsp509I     TspRI       
TthHB8I     
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