Restriction map for pF1KE0678
 (Linear) (Six-base) MAPSORT of: KIEE0678.seq  Check: 9547  from: 1  to: 1188



KIEE0678.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     341    1188 
   Size:        341     847
 
AflIII A'CryG_T

Cuts at:      0     284     707    1033    1188 
   Size:        284     423     326     155

  Fragments arranged by size: 

                 423     326     284     155
 
AhdI GACnn_n'nnGTC

Cuts at:      0     618    1188 
   Size:        618     570
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     271     303    1188 
   Size:        271      32     885

  Fragments arranged by size: 

                 885     271      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     231    1188 
   Size:        231     957
 
ApaLI G'TGCA_C

Cuts at:      0     478    1188 
   Size:        478     710
 
ApoI r'AATT_y

Cuts at:      0      71    1188 
   Size:         71    1117
 
BanI G'GyrC_C

Cuts at:      0     544     561    1188 
   Size:        544      17     627

  Fragments arranged by size: 

                 627     544      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     195     287    1030    1188 
   Size:        195      92     743     158

  Fragments arranged by size: 

                 743     195     158      92
 
Bme1580I G_kGCm'C

Cuts at:      0     476     482    1188 
   Size:        476       6     706

  Fragments arranged by size: 

                 706     476       6
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     864    1188 
   Size:        864     324
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     253     564     663    1188 
   Size:        199      54     311      99     525

  Fragments arranged by size: 

                 525     311     199      99      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     958    1188 
   Size:        958     230
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     311     654    1188 
   Size:        311     343     534

  Fragments arranged by size: 

                 534     343     311
 
BsaAI yAC'GTr

Cuts at:      0     477    1188 
   Size:        477     711
 
BseYI C'CCAG_C

Cuts at:      0     309    1188 
   Size:        309     879
 
BsiHKAI G_wGCw'C

Cuts at:      0     239     482    1052    1188 
   Size:        239     243     570     136

  Fragments arranged by size: 

                 570     243     239     136
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     709    1188 
   Size:        709     479
 
Bsp1286I G_dGCh'C

Cuts at:      0     239     476     482    1052    1188 
   Size:        239     237       6     570     136

  Fragments arranged by size: 

                 570     239     237     136       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     329     341     416    1188 
   Size:        329      12      75     772

  Fragments arranged by size: 

                 772     329      75      12
 
BsrDI GCAATG_nn'

Cuts at:      0     155     192     497    1188 
   Size:        155      37     305     691

  Fragments arranged by size: 

                 691     305     155      37
 
BsrGI T'GTAC_A

Cuts at:      0     117     702    1188 
   Size:        117     585     486

  Fragments arranged by size: 

                 585     486     117
 
BtgI C'CryG_G

Cuts at:      0     536    1188 
   Size:        536     652
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     520    1188 
   Size:        520     668
 
DraIII CAC_nnn'GTG

Cuts at:      0    1162    1188 
   Size:       1162      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     379     868    1188 
   Size:        379     489     320

  Fragments arranged by size: 

                 489     379     320
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     253     564     663    1188 
   Size:        199      54     311      99     525

  Fragments arranged by size: 

                 525     311     199      99      54
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     902    1188 
   Size:        902     286
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1069    1188 
   Size:       1069     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     604     636     651     683    1188 
   Size:        604      32      15      32     505

  Fragments arranged by size: 

                 604     505      32      32      15
 
HincII GTy'rAC

Cuts at:      0      54     612    1188 
   Size:         54     558     576

  Fragments arranged by size: 

                 576     558      54
 
MslI CAynn'nnrTG

Cuts at:      0     289     295    1188 
   Size:        289       6     893

  Fragments arranged by size: 

                 893     289       6
 
NcoI C'CATG_G

Cuts at:      0     536    1188 
   Size:        536     652
 
NdeI CA'TA_TG

Cuts at:      0     639    1188 
   Size:        639     549
 
NspI r_CATG'y

Cuts at:      0     288    1037    1188 
   Size:        288     749     151

  Fragments arranged by size: 

                 749     288     151
 
PasI CC'CwG_GG

Cuts at:      0     515     624    1188 
   Size:        515     109     564

  Fragments arranged by size: 

                 564     515     109
 
PciI A'CATG_T

Cuts at:      0     284    1033    1188 
   Size:        284     749     155

  Fragments arranged by size: 

                 749     284     155
 
PmlI CAC'GTG

Cuts at:      0     477    1188 
   Size:        477     711
 
PstI C_TGCA'G

Cuts at:      0     413    1188 
   Size:        413     775
 
SapI GCTCTTCn'nnn_

Cuts at:      0     379    1188 
   Size:        379     809
 
ScaI AGT'ACT

Cuts at:      0     684    1188 
   Size:        684     504
 
SfcI C'TryA_G

Cuts at:      0     409    1188 
   Size:        409     779
 
SmlI C'TyrA_G

Cuts at:      0     937    1188 
   Size:        937     251
 
SspI AAT'ATT

Cuts at:      0     453    1188 
   Size:        453     735
 
StyI C'CwwG_G

Cuts at:      0     536    1136    1188 
   Size:        536     600      52

  Fragments arranged by size: 

                 600     536      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     301    1188 
   Size:        301     887
 
TatI w'GTAC_w

Cuts at:      0     117     155     682     702    1188 
   Size:        117      38     527      20     486

  Fragments arranged by size: 

                 527     486     117      38      20
 
Tth111I GACn'n_nGTC

Cuts at:      0    1060    1188 
   Size:       1060     128
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     555    1188 
   Size:        555     633
 
XmnI GAAnn'nnTTC

Cuts at:      0     373    1072    1188 
   Size:        373     699     116

  Fragments arranged by size: 

                 699     373     116
 

 Enzymes that do cut:

     AarI   AflIII     AhdI     AloI    AlwNI    ApaLI     ApoI     BanI
     BbsI Bme1580I     BmrI     BpmI    BpuEI     BsaI    BsaAI    BseYI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI    BsrGI     BtgI    BtgZI
   DraIII     EarI  Eco57MI    EcoNI     FalI    Hin4I   HincII     MslI
     NcoI     NdeI     NspI     PasI     PciI     PmlI     PstI     SapI
     ScaI     SfcI     SmlI     SspI     StyI    TaqII     TatI  Tth111I
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     NsiI     PacI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TstI     XbaI     XhoI
     XmaI     ZraI
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