Restriction map for pF1KB8532
##################################
# NO CUT ENZYMES (   97 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AhdI        AlwNI       AocI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        BciVI       BclI        BfrI        BglII       
BsaBI       BseAI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoT22I     FbaI        FseI        
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccI          448  GTCGAC               GT^[AC][GT]AC
AgeI         2184  ACCGGT               A^CCGGT
Alw44I        708  GTGCAC               G^TGCAC
Aor51HI      1670  AGCGCT               AGC^GCT
ApaLI         708  GTGCAC               G^TGCAC
AviII         951  TGCGCA               TGC^GCA
AvrII         999  CCTAGG               C^CTAGG
BalI          395  TGGCCA               TGG^CCA
BamHI        2404  GGATCC               G^GATCC
BbeI         1987  GGCGCC               GGCGC^C
BbrPI        1366  CACGTG               CAC^GTG
BbvCI        1447  GCTGAGG              CCTCAGC(-5/-2)
BglI         2453  GCCGAAGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          999  CCTAGG               C^CTAGG
BlpI         1858  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     1858  GCTAAGC              GC^T[ACGT]AGC
BsaAI        1366  CACGTG               [CT]AC^GT[GA]
BsaWI        2184  ACCGGT               [AT]^CCGG[AT]
BsgI         1363  CTGCAC               GTGCAG(16/14)
BsrBI         115  GAGCGG               CCGCTC(-3/-3)
BstAPI        957  GCACGCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        1858  GCTAAGC              GC^T[ACGT]AGC
DraIII        944  CACAGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1670  AGCGCT               AGC^GCT
EcoRV         937  GATATC               GAT^ATC
FspI          951  TGCGCA               TGC^GCA
HindIII      1771  AAGCTT               A^AGCTT
KasI         1987  GGCGCC               G^GCGCC
MluNI         395  TGGCCA               TGG^CCA
MscI          395  TGGCCA               TGG^CCA
MseI         1376  TTAA                 T^TAA
MspA1I       1231  CAGCTG               C[AC]G^C[GT]G
NarI         1987  GGCGCC               GG^CGCC
PinAI        2184  ACCGGT               A^CCGGT
PmaCI        1366  CACGTG               CAC^GTG
PmlI         1366  CACGTG               CAC^GTG
PstI         1246  CTGCAG               CTGCA^G
PvuII        1231  CAGCTG               CAG^CTG
SalI          448  GTCGAC               G^TCGAC
SfoI         1987  GGCGCC               GGC^GCC
SgrAI        2183  CACCGGTG             C[GA]^CCGG[CT]G
TfiI         1167  GATTC                G^A[AT]TC
Tru9I        1376  TTAA                 T^TAA
XbaI          915  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII         715  CGCG                 CG^CG
             1969  CGCG                
ApaI         1273  GGGCCC               GGGCC^C
             1339  GGGCCC              
BanI         1987  GGCGCC               G^G[CT][GA]CC
             2187  GGTGCC              
BbsI          240  GAAGAC               GAAGAC(2/6)
              421  GAAGAC              
BcgI          450  CGACGATGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1319  GCAATTCATTCG        
BpuAI         240  GAAGAC               GAAGAC(2/6)
              421  GAAGAC              
BsaI         1453  GAGACC               GGTCTC(1/5)
             1648  GAGACC              
BsrFI        2014  GCCGGT               [GA]^CCGG[CT]
             2184  ACCGGT              
BstUI         715  CGCG                 CG^CG
             1969  CGCG                
BstXI        1298  CCATGTCTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1710  CCAAGGTGCTGG        
Cfr10I       2014  GCCGGT               [GA]^CCGG[CT]
             2184  ACCGGT              
DsaI         1995  CCATGG               C^C[GA][CT]GG
             2346  CCATGG              
EcoNI         306  CCTACTCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              372  CCTGGACAAGG         
HincII        448  GTCGAC               GT[CT]^[GA]AC
              571  GTCAAC              
HindII        448  GTCGAC               GT[CT]^[GA]AC
              571  GTCAAC              
HphI         2167  GGTGA                GGTGA(8/7)
             2182  TCACC               
MvnI          715  CGCG                 CG^CG
             1969  CGCG                
NcoI         1995  CCATGG               C^CATGG
             2346  CCATGG              
PaeR7I        751  CTCGAG               C^TCGAG
             1425  CTCGAG              
PflMI          36  CCATGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               93  CCAGAACATGG         
PpuMI         701  GGGACCT              [GA]G^G[AT]CC[CT]
              729  GGGTCCC             
SacI          754  GAGCTC               GAGCT^C
             1636  GAGCTC              
SmaI         2389  CCCGGG               CCC^GGG
             2592  CCCGGG              
StuI         1331  AGGCCT               AGG^CCT
             1742  AGGCCT              
Tsp45I       2168  GTGAC                ^GT[GC]AC
             2505  GTCAC               
Van91I         36  CCATGTGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
               93  CCAGAACATGG         
XhoI          751  CTCGAG               C^TCGAG
             1425  CTCGAG              
XmaCI        2389  CCCGGG               C^CCGGG
             2592  CCCGGG              
XmaI         2389  CCCGGG               C^CCGGG
             2592  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   88 enzymes) #
#########################################

AciI        AcsI        AcyI        AfaI        AluI        
AlwI        ApoI        AspHI       AvaI        AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaHI       BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TseI        Tsp509I     TspRI       
TthHB8I     XcmI        XhoII       
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