Restriction map for pF1KB8532
 (Linear) (Six-base) MAPSORT of: KIBB8532.seq  Check: 9664  from: 1  to: 2694



KIBB8532.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AccI GT'mk_AC

Cuts at:      0     449    2694 
   Size:        449    2245
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688    1255    1433    2694 
   Size:        688     567     178    1261

  Fragments arranged by size: 

                1261     688     567     178
 
AfeI AGC'GCT

Cuts at:      0    1672    2694 
   Size:       1672    1022
 
AgeI A'CCGG_T

Cuts at:      0    2184    2694 
   Size:       2184     510
 
AleI CACnn'nnGTG

Cuts at:      0     715    2694 
   Size:        715    1979
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     733     868    2694 
   Size:        733     135    1826

  Fragments arranged by size: 

                1826     733     135
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      88     120    2572    2604    2694 
   Size:         88      32    2452      32      90

  Fragments arranged by size: 

                2452      90      88      32      32
 
ApaI G_GGCC'C

Cuts at:      0    1277    1343    2694 
   Size:       1277      66    1351

  Fragments arranged by size: 

                1351    1277      66
 
ApaLI G'TGCA_C

Cuts at:      0     708    2694 
   Size:        708    1986
 
ApoI r'AATT_y

Cuts at:      0      14    1849    2428    2694 
   Size:         14    1835     579     266

  Fragments arranged by size: 

                1835     579     266      14
 
AvaI C'yCGr_G

Cuts at:      0     751    1425    2250    2389    2592    2694 
   Size:        751     674     825     139     203     102

  Fragments arranged by size: 

                 825     751     674     203     139     102
 
AvrII C'CTAG_G

Cuts at:      0     999    2694 
   Size:        999    1695
 
BamHI G'GATC_C

Cuts at:      0    2404    2694 
   Size:       2404     290
 
BanI G'GyrC_C

Cuts at:      0    1987    2187    2694 
   Size:       1987     200     507

  Fragments arranged by size: 

                1987     507     200
 
BanII G_rGCy'C

Cuts at:      0     758    1196    1277    1343    1628    1640    1824    2299 
   Size:        758     438      81      66     285      12     184     475

Cuts at:   2299    2694 
   Size:        395

  Fragments arranged by size: 

                 758     475     438     395     285     184      81      66
                  12
 
BbeI G_GCGC'C

Cuts at:      0    1991    2694 
   Size:       1991     703
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     247     428    2694 
   Size:        247     181    2266

  Fragments arranged by size: 

                2266     247     181
 
BbvCI CC'TCA_GC

Cuts at:      0    1448    2694 
   Size:       1448    1246
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     439     473    1308    1342    2694 
   Size:        439      34     835      34    1352

  Fragments arranged by size: 

                1352     835     439      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2459    2694 
   Size:       2459     235
 
BlpI GC'TnA_GC

Cuts at:      0    1859    2694 
   Size:       1859     835
 
Bme1580I G_kGCm'C

Cuts at:      0     203     712     722    1277    1343    1913    2192    2694 
   Size:        203     509      10     555      66     570     279     502

  Fragments arranged by size: 

                 570     555     509     502     279     203      66      10
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1048    1280    1483    1588    1819    2694 
   Size:       1048     232     203     105     231     875

  Fragments arranged by size: 

                1048     875     232     231     203     105
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1659    2694 
   Size:       1659    1035
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     349     575     847    2694 
   Size:        103     246     226     272    1847

  Fragments arranged by size: 

                1847     272     246     226     103
 
Bpu10I CC'TnA_GC

Cuts at:      0    1448    2478    2694 
   Size:       1448    1030     216

  Fragments arranged by size: 

                1448    1030     216
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     717    1612    2694 
   Size:        295     422     895    1082

  Fragments arranged by size: 

                1082     895     422     295
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1447    1642    2694 
   Size:       1447     195    1052

  Fragments arranged by size: 

                1447    1052     195
 
BsaAI yAC'GTr

Cuts at:      0    1368    2694 
   Size:       1368    1326
 
BsaHI Gr'CG_yC

Cuts at:      0     622     838    1988    2694 
   Size:        622     216    1150     706

  Fragments arranged by size: 

                1150     706     622     216
 
BsaWI w'CCGG_w

Cuts at:      0    2184    2694 
   Size:       2184     510
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1126    1156    2359    2389    2572    2602    2694 
   Size:       1126      30    1203      30     183      30      92

  Fragments arranged by size: 

                1203    1126     183      92      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      91    1138    1150    1195    1465    2130    2532    2694 
   Size:         91    1047      12      45     270     665     402     162

  Fragments arranged by size: 

                1047     665     402     270     162      91      45      12
            
 
BseYI C'CCAG_C

Cuts at:      0     229    1147    2020    2203    2623    2694 
   Size:        229     918     873     183     420      71

  Fragments arranged by size: 

                 918     873     420     229     183      71
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1348    2694 
   Size:       1348    1346
 
BsiHKAI G_wGCw'C

Cuts at:      0     251     712     758    1640    2694 
   Size:        251     461      46     882    1054

  Fragments arranged by size: 

                1054     882     461     251      46
 
BsmI GAATG_Cn'

Cuts at:      0      51    1215    2016    2094    2109    2694 
   Size:         51    1164     801      78      15     585

  Fragments arranged by size: 

                1164     801     585      78      51      15
 
Bsp1286I G_dGCh'C

Cuts at:      0     203     251     712     722     758    1196    1277    1343 
   Size:        203      48     461      10      36     438      81      66

Cuts at:   1343    1628    1640    1824    1913    2192    2299    2694 
   Size:        285      12     184      89     279     107     395

  Fragments arranged by size: 

                 461     438     395     285     279     203     184     107
                  89      81      66      48      36      12      10
 
BsrBI CCG'CTC

Cuts at:      0     117    2694 
   Size:        117    2577
 
BsrFI r'CCGG_y

Cuts at:      0    2014    2184    2694 
   Size:       2014     170     510

  Fragments arranged by size: 

                2014     510     170
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     963    2694 
   Size:        963    1731
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1305    1717    2694 
   Size:       1305     412     977

  Fragments arranged by size: 

                1305     977     412
 
BstYI r'GATC_y

Cuts at:      0     324    1073    2404    2694 
   Size:        324     749    1331     290

  Fragments arranged by size: 

                1331     749     324     290
 
BtgI C'CryG_G

Cuts at:      0    1995    2346    2694 
   Size:       1995     351     348

  Fragments arranged by size: 

                1995     351     348
 
BtsI GCAGTG_nn'

Cuts at:      0     961    1478    1538    2694 
   Size:        961     517      60    1156

  Fragments arranged by size: 

                1156     961     517      60
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2421    2456    2694 
   Size:       2421      35     238

  Fragments arranged by size: 

                2421     238      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     949    2694 
   Size:        949    1745
 
EaeI y'GGCC_r

Cuts at:      0     383     395     782     981    1355    1370    2601    2694 
   Size:        383      12     387     199     374      15    1231      93

  Fragments arranged by size: 

                1231     387     383     374     199      93      15      12
            
 
EarI CTCTTCn'nnn_

Cuts at:      0    1609    1810    1935    2694 
   Size:       1609     201     125     759

  Fragments arranged by size: 

                1609     759     201     125
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2417    2537    2694 
   Size:       2417     120     157

  Fragments arranged by size: 

                2417     157     120
 
EcoICRI GAG'CTC

Cuts at:      0     756    1638    2694 
   Size:        756     882    1056

  Fragments arranged by size: 

                1056     882     756
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     103     349     575     688     847    1255    1433    2694 
   Size:        103     246     226     113     159     408     178    1261

  Fragments arranged by size: 

                1261     408     246     226     178     159     113     103
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     310     376    2694 
   Size:        310      66    2318

  Fragments arranged by size: 

                2318     310      66
 
EcoO109I rG'GnC_Cy

Cuts at:      0     300     702     730     810    1340    1891    2694 
   Size:        300     402      28      80     530     551     803

  Fragments arranged by size: 

                 803     551     530     402     300      80      28
 
EcoRV GAT'ATC

Cuts at:      0     939    2694 
   Size:        939    1755
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     336    2694 
   Size:        336    2358
 
FspI TGC'GCA

Cuts at:      0     953    2694 
   Size:        953    1741
 
HaeII r_GCGC'y

Cuts at:      0     124    1674    1991    2694 
   Size:        124    1550     317     703

  Fragments arranged by size: 

                1550     703     317     124
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1126    1158    2694 
   Size:       1126      32    1536

  Fragments arranged by size: 

                1536    1126      32
 
HincII GTy'rAC

Cuts at:      0     450     573    2694 
   Size:        450     123    2121

  Fragments arranged by size: 

                2121     450     123
 
HindIII A'AGCT_T

Cuts at:      0    1771    2694 
   Size:       1771     923
 
KasI G'GCGC_C

Cuts at:      0    1987    2694 
   Size:       1987     707
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      91     161     654    1748    2432    2694 
   Size:         91      70     493    1094     684     262

  Fragments arranged by size: 

                1094     684     493     262      91      70
 
MscI TGG'CCA

Cuts at:      0     397    2694 
   Size:        397    2297
 
MslI CAynn'nnrTG

Cuts at:      0     196     715     850    1303    2694 
   Size:        196     519     135     453    1391

  Fragments arranged by size: 

                1391     519     453     196     135
 
MspA1I CmG'CkG

Cuts at:      0    1233    2694 
   Size:       1233    1461
 
NarI GG'CG_CC

Cuts at:      0    1988    2694 
   Size:       1988     706
 
NcoI C'CATG_G

Cuts at:      0    1995    2346    2694 
   Size:       1995     351     348

  Fragments arranged by size: 

                1995     351     348
 
PasI CC'CwG_GG

Cuts at:      0    2500    2694 
   Size:       2500     194
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42      99    2694 
   Size:         42      57    2595

  Fragments arranged by size: 

                2595      57      42
 
PfoI T'CCnGG_A

Cuts at:      0     371    2694 
   Size:        371    2323
 
PmlI CAC'GTG

Cuts at:      0    1368    2694 
   Size:       1368    1326
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2572    2604    2694 
   Size:       2572      32      90

  Fragments arranged by size: 

                2572      90      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     702     730    2694 
   Size:        702      28    1964

  Fragments arranged by size: 

                1964     702      28
 
PspOMI G'GGCC_C

Cuts at:      0    1273    1339    2694 
   Size:       1273      66    1355

  Fragments arranged by size: 

                1355    1273      66
 
PspXI vC'TCGA_Gb

Cuts at:      0    1425    2694 
   Size:       1425    1269
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    2671    2694 
   Size:       2671      23
 
PstI C_TGCA'G

Cuts at:      0    1250    2694 
   Size:       1250    1444
 
PvuII CAG'CTG

Cuts at:      0    1233    2694 
   Size:       1233    1461
 
SacI G_AGCT'C

Cuts at:      0     758    1640    2694 
   Size:        758     882    1054

  Fragments arranged by size: 

                1054     882     758
 
SalI G'TCGA_C

Cuts at:      0     448    2694 
   Size:        448    2246
 
SanDI GG'GwC_CC

Cuts at:      0     730    2694 
   Size:        730    1964
 
SfcI C'TryA_G

Cuts at:      0    1246    2067    2079    2301    2436    2610    2694 
   Size:       1246     821      12     222     135     174      84

  Fragments arranged by size: 

                1246     821     222     174     135      84      12
 
SfoI GGC'GCC

Cuts at:      0    1989    2694 
   Size:       1989     705
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2184    2694 
   Size:       2184     510
 
SmaI CCC'GGG

Cuts at:      0    2391    2594    2694 
   Size:       2391     203     100

  Fragments arranged by size: 

                2391     203     100
 
SmlI C'TyrA_G

Cuts at:      0     310     696     751    1425    1627    2694 
   Size:        310     386      55     674     202    1067

  Fragments arranged by size: 

                1067     674     386     310     202      55
 
StuI AGG'CCT

Cuts at:      0    1333    1744    2694 
   Size:       1333     411     950

  Fragments arranged by size: 

                1333     950     411
 
StyI C'CwwG_G

Cuts at:      0     617     999    1710    1995    2163    2346    2550    2694 
   Size:        617     382     711     285     168     183     204     144

  Fragments arranged by size: 

                 711     617     382     285     204     183     168     144
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     787     807    1471    1860    2694 
   Size:        787      20     664     389     834

  Fragments arranged by size: 

                 834     787     664     389      20
 
TatI w'GTAC_w

Cuts at:      0    1250    2687    2694 
   Size:       1250    1437       7

  Fragments arranged by size: 

                1437    1250       7
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     499     531     714     746    2094    2126    2694 
   Size:        499      32     183      32    1348      32     568

  Fragments arranged by size: 

                1348     568     499     183      32      32      32
 
XbaI T'CTAG_A

Cuts at:      0     915    2694 
   Size:        915    1779
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     792    1365    1731    2611    2694 
   Size:        792     573     366     880      83

  Fragments arranged by size: 

                 880     792     573     366      83
 
XhoI C'TCGA_G

Cuts at:      0     751    1425    2694 
   Size:        751     674    1269

  Fragments arranged by size: 

                1269     751     674
 
XmaI C'CCGG_G

Cuts at:      0    2389    2592    2694 
   Size:       2389     203     102

  Fragments arranged by size: 

                2389     203     102
 

 Enzymes that do cut:

     AccI     AcuI     AfeI     AgeI     AleI     AlfI     AloI     ApaI
    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI     BglI     BlpI Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrFI
   BstAPI    BstXI    BstYI     BtgI     BtsI    CspCI   DraIII     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     KasI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     PasI    PflMI     PfoI     PmlI
     PpiI    PpuMI   PspOMI    PspXI     PsrI     PstI    PvuII     SacI
     SalI    SanDI     SfcI     SfoI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TatI     TstI     XbaI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII   AflIII     AhdI    AlwNI
     AscI     AseI    AsiSI     BaeI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmtI    BsaBI    BsiEI    BsiWI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI     DrdI     EagI    EcoRI     FseI
    FspAI     HpaI     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
    PshAI     PsiI     PvuI    RsrII    SacII     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI  Tth111I     XmnI     ZraI
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