Restriction map for pF1KB6407
##################################
# NO CUT ENZYMES (  152 enzymes) #
##################################

Acc65I      AccI        AccII       AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AviII       AvrII       BanII       BbeI        BbrPI       
BbsI        BbvCI       BciVI       BclI        BcnI        
BfaI        BfrI        BglI        BlnI        BpuAI       
BsaBI       BsaI        BseRI       BsgI        BsiEI       
BsiWI       BsmI        BspDI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BssHII      BssKI       
Bst1107I    BstAPI      BstBI       BstEII      BstNI       
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
Cfr10I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRII      EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MamI        MfeI        MluI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScrFI       
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        StuI        SwaI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AatII         502  GACGTC               GACGT^C
AccIII         47  TCCGGA               T^CCGGA
AcsI          130  AAATTT               [GA]^AATT[CT]
Aor51HI       704  AGCGCT               AGC^GCT
ApoI          130  AAATTT               [GA]^AATT[CT]
AvaII         112  GGTCC                G^G[AT]CC
BamHI          44  GGATCC               G^GATCC
BanI          780  GGTGCC               G^G[CT][GA]CC
BcgI           53  GCAGACGCCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI          47  TCCGGA               [AT]^CCGG[AT]
BseAI          47  TCCGGA               T^CCGGA
BsmBI         641  GAGACG               CGTCTC(1/5)
Bsp1407I      450  TGTACA               T^GTACA
BspEI          47  TCCGGA               T^CCGGA
BsrBI         754  GAGCGG               CCGCTC(-3/-3)
BsrGI         450  TGTACA               T^GTACA
BssSI         700  CACGAG               CACGAG(-5/-1)
BstF5I        599  GGATG                GGATG(2/0)
DsaI          776  CCATGG               C^C[GA][CT]GG
Eco47III      704  AGCGCT               AGC^GCT
FauI          756  GCGGG                CCCGC(4/6)
FokI          599  GGATG                GGATG(9/13)
HapII          48  CCGG                 C^CGG
HgaI           56  GACGC                GACGC(5/10)
HpaII          48  CCGG                 C^CGG
HphI          613  GGTGA                GGTGA(8/7)
MaeIII        175  GTTAC                ^GT[ACGT]AC
MroI           47  TCCGGA               T^CCGGA
MseI           14  TTAA                 T^TAA
MslI          774  CACCATGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           48  CCGG                 C^CGG
NcoI          776  CCATGG               C^CATGG
NdeI          479  CATATG               CA^TATG
NspI          231  ACATGC               [GA]CATG^[CT]
PleI          518  GAGTC                GAGTC(4/5)
ScaI          315  AGTACT               AGT^ACT
SmlI          292  CTTGAG               C^T[CT][GA]AG
SspBI         450  TGTACA               T^GTACA
Tru9I          14  TTAA                 T^TAA
TspRI         384  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcyI           56  GACGCC               G[GA]^CG[CT]C
              502  GACGTC              
AspHI         771  GAGCAC               G[AT]GC[AT]^C
              913  GAGCAC              
AvaI           61  CTCGGG               C^[CT]CG[GA]G
              638  CCCGAG              
BalI          380  TGGCCA               TGG^CCA
              683  TGGCCA              
BglII         288  AGATCT               A^GATCT
              907  AGATCT              
BlpI          540  GCTAAGC              GC^T[ACGT]AGC
              768  GCTGAGC             
Bpu1102I      540  GCTAAGC              GC^T[ACGT]AGC
              768  GCTGAGC             
BsaAI          37  TACGTG               [CT]AC^GT[GA]
              573  TACGTG              
BsaHI          56  GACGCC               G[GA]^CG[CT]C
              502  GACGTC              
BsiHKAI       771  GAGCAC               G[AT]GC[AT]^C
              913  GAGCAC              
BsmFI         500  GGGAC                GGGAC(10/14)
              834  GGGAC               
BsoBI          61  CTCGGG               C^[CT]CG[GA]G
              638  CCCGAG              
CelII         540  GCTAAGC              GC^T[ACGT]AGC
              768  GCTGAGC             
Cfr13I        112  GGTCC                G^G[ACGT]CC
              758  GGGCC               
Hin1I          56  GACGCC               G[GA]^CG[CT]C
              502  GACGTC              
HindIII        98  AAGCTT               A^AGCTT
              874  AAGCTT              
MboII         195  GAAGA                GAAGA(8/7)
              744  GAAGA               
MluNI         380  TGGCCA               TGG^CCA
              683  TGGCCA              
MscI          380  TGGCCA               TGG^CCA
              683  TGGCCA              
MwoI          676  GCTGCCTTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              696  GCAGCACGAGC         
NlaIV          44  GGATCC               GG[ACGT]^[ACGT]CC
              780  GGTGCC              
Sau96I        112  GGTCC                G^G[ACGT]CC
              758  GGGCC               
SfcI          712  CTACAG               C^T[GA][CT]AG
              903  CTACAG              
Tsp509I        10  AATT                 ^AATT
              131  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   42 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        BbvI        
BmyI        BpmI        BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BsrI        BstYI       Cac8I       
CfoI        DdeI        DpnII       EaeI        EcoT14I     
Fnu4HI      HaeII       HaeIII      HhaI        HinP1I      
HinfI       ItaI        MaeII       MboI        MflI        
MnlI        NdeII       NlaIII      RsaI        Sau3AI      
SfaNI       StyI        TaqI        TfiI        TseI        
TthHB8I     XhoII       
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