Restriction map for pF1KB6758
 (Circular) (Six-base) MAPSORT of: pF1KB6758.seq  Check: 7880  from: 1  to: 3554



pF1KB6758  3554 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:22  ..

AccI GT'mk_AC

Cuts at:    592     592 
   Size:       3554
 
Acc65I G'GTAC_C

Cuts at:    570     570 
   Size:       3554
 
AclI AA'CG_TT

Cuts at:   3394    3394 
   Size:       3554
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    184    1379    1811    2558    2826     184 
   Size:       1195     432     747     268     912

  Fragments arranged by size: 

                1195     912     747     432     268
 
AflIII A'CryG_T

Cuts at:    906    2010    2987     906 
   Size:       1104     977    1473

  Fragments arranged by size: 

                1473    1104     977
 
AgeI A'CCGG_T

Cuts at:   1942    1942 
   Size:       3554
 
AlwNI CAG_nnn'CTG

Cuts at:    859    2426     859 
   Size:       1567    1987
 
ApaLI G'TGCA_C

Cuts at:   2324    2324 
   Size:       3554
 
ApoI r'AATT_y

Cuts at:    558    2907    3163     558 
   Size:       2349     256     949

  Fragments arranged by size: 

                2349     949     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3554
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3554
 
AvaI C'yCGr_G

Cuts at:    574     574 
   Size:       3554
 
BamHI G'GATC_C

Cuts at:    579     579 
   Size:       3554
 
BanI G'GyrC_C

Cuts at:    511     570     984    1232    1267     511 
   Size:         59     414     248      35    2798

  Fragments arranged by size: 

                2798     414     248      59      35
 
BanII G_rGCy'C

Cuts at:    568    1598     568 
   Size:       1030    2524
 
BbeI G_GCGC'C

Cuts at:    988    1236     988 
   Size:        248    3306
 
BbsI GAAGACnn'nnnn_

Cuts at:    145     145 
   Size:       3554
 
BbvCI CC'TCA_GC

Cuts at:    483     483 
   Size:       3554
 
BciVI GTATCCnnnnn_n'

Cuts at:   1452    2213    3554    1452 
   Size:        761    1341    1452

  Fragments arranged by size: 

                1452    1341     761
 
BclI T'GATC_A

Cuts at:   1074    1074 
   Size:       3554
 
BglII A'GATC_T

Cuts at:   1069    3106    1069 
   Size:       2037    1517
 
BlpI GC'TnA_GC

Cuts at:    223     666     223 
   Size:        443    3111
 
Bme1580I G_kGCm'C

Cuts at:    516    1179    1272    2328     516 
   Size:        663      93    1056    1742

  Fragments arranged by size: 

                1742    1056     663      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    766     847    1182    3326     766 
   Size:         81     335    2144     994

  Fragments arranged by size: 

                2144     994     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1921    1921 
   Size:       3554
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    305     305 
   Size:       3554
 
Bpu10I CC'TnA_GC

Cuts at:    483     483 
   Size:       3554
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    730    1609    2101    2399     730 
   Size:        879     492     298    1885

  Fragments arranged by size: 

                1885     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3258    3258 
   Size:       3554
 
BsaAI yAC'GTr

Cuts at:    907     907 
   Size:       3554
 
BsaBI GATnn'nnATC

Cuts at:      6    1068    3105       6 
   Size:       1062    2037     455

  Fragments arranged by size: 

                2037    1062     455
 
BsaHI Gr'CG_yC

Cuts at:    985    1233    1935    3431     985 
   Size:        248     702    1496    1108

  Fragments arranged by size: 

                1496    1108     702     248
 
BsaWI w'CCGG_w

Cuts at:    743    1264    1942    2216    2363    2774     743 
   Size:        521     678     274     147     411    1523

  Fragments arranged by size: 

                1523     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    303     333     508     538     303 
   Size:         30     175      30    3319

  Fragments arranged by size: 

                3319     175      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    130     130 
   Size:       3554
 
BseYI C'CCAG_C

Cuts at:    499     979    1737    2314    2810     499 
   Size:        480     758     577     496    1243

  Fragments arranged by size: 

                1243     758     577     496     480
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    214     214 
   Size:       3554
 
BsiEI CG_ry'CG

Cuts at:    104    1096    1142    2350     104 
   Size:        992      46    1208    1308

  Fragments arranged by size: 

                1308    1208     992      46
 
BsiHKAI G_wGCw'C

Cuts at:    568    1346    1536    2328     568 
   Size:        778     190     792    1794

  Fragments arranged by size: 

                1794     792     778     190
 
BsmI GAATG_Cn'

Cuts at:    422     422 
   Size:       3554
 
BsmBI CGTCTCn'nnnn_

Cuts at:    379     379 
   Size:       3554
 
Bsp1286I G_dGCh'C

Cuts at:    516     568    1179    1272    1346    1536    1598    2328     516 
   Size:         52     611      93      74     190      62     730    1742

  Fragments arranged by size: 

                1742     730     611     190      93      74      62      52
            
 
BspEI T'CCGG_A

Cuts at:   2774    2774 
   Size:       3554
 
BspHI T'CATG_A

Cuts at:   2730    2730 
   Size:       3554
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    604     818    1120    1501     604 
   Size:        214     302     381    2657

  Fragments arranged by size: 

                2657     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1846    1900    3384    3549      50 
   Size:       1796      54    1484     165      55

  Fragments arranged by size: 

                1796    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1466    1466 
   Size:       3554
 
BsrFI r'CCGG_y

Cuts at:   1552    1733    1942    1552 
   Size:        181     209    3164

  Fragments arranged by size: 

                3164     209     181
 
BssSI C'ACGA_G

Cuts at:   1825    2183    1825 
   Size:        358    3196
 
BstAPI GCAn_nnn'nTGC

Cuts at:    452     931     452 
   Size:        479    3075
 
BstBI TT'CG_AA

Cuts at:   1915    1915 
   Size:       3554
 
BstXI CCAn_nnnn'nTGG

Cuts at:    499     499 
   Size:       3554
 
BstYI r'GATC_y

Cuts at:      1     579    1046    1069    1404    1650    2662    2748    2760 
   Size:        578     467      23     335     246    1012      86      12

Cuts at:   2760    3106       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     578     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    188     188 
   Size:       3554
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1504    1685    1848    1504 
   Size:        181     163    3210

  Fragments arranged by size: 

                3210     181     163
 
BtsI GCAGTG_nn'

Cuts at:    427     797     427 
   Size:        370    3184
 
DraI TTT'AAA

Cuts at:    553     553 
   Size:       3554
 
DrdI GACnn_nn'nnGTC

Cuts at:   1260    2118    1260 
   Size:        858    2696
 
EaeI y'GGCC_r

Cuts at:    170    1139    1313    1704    1731    3076     170 
   Size:        969     174     391      27    1345     648

  Fragments arranged by size: 

                1345     969     648     391     174      27
 
EagI C'GGCC_G

Cuts at:   1139    1139 
   Size:       3554
 
EarI CTCTTCn'nnn_

Cuts at:   1577    1787    1577 
   Size:        210    3344
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1762    2072    2218    3364    1762 
   Size:        310     146    1146    1952

  Fragments arranged by size: 

                1952    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    566     566 
   Size:       3554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    184     305    1379    1811    2558    2826     184 
   Size:        121    1074     432     747     268     912

  Fragments arranged by size: 

                1074     912     747     432     268     121
 
EcoO109I rG'GnC_Cy

Cuts at:    693     693 
   Size:       3554
 
EcoRI G'AATT_C

Cuts at:    558     558 
   Size:       3554
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1573    1573 
   Size:       3554
 
FspI TGC'GCA

Cuts at:   1335    1335 
   Size:       3554
 
HaeII r_GCGC'y

Cuts at:    988    1236    2258    3237     988 
   Size:        248    1022     979    1305

  Fragments arranged by size: 

                1305    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    376     378     408     410    1396    1426    1428    1458     376 
   Size:          2      30       2     986      30       2      30    2472

  Fragments arranged by size: 

                2472     986      30      30      30       2       2       2
            
 
HincII GTy'rAC

Cuts at:    593     593 
   Size:       3554
 
KasI G'GCGC_C

Cuts at:    984    1232     984 
   Size:        248    3306
 
KpnI G_GTAC'C

Cuts at:    574     574 
   Size:       3554
 
MluI A'CGCG_T

Cuts at:   2987    2987 
   Size:       3554
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2225    2409    2225 
   Size:        184    3370
 
MscI TGG'CCA

Cuts at:   1315    1315 
   Size:       3554
 
MslI CAynn'nnrTG

Cuts at:   1543    1670    1543 
   Size:        127    3427
 
MspA1I CmG'CkG

Cuts at:    222     665     979    1339    2352    2597     222 
   Size:        443     314     360    1013     245    1179

  Fragments arranged by size: 

                1179    1013     443     360     314     245
 
NaeI GCC'GGC

Cuts at:   1735    1735 
   Size:       3554
 
NarI GG'CG_CC

Cuts at:    985    1233     985 
   Size:        248    3306
 
NgoMIV G'CCGG_C

Cuts at:   1733    1733 
   Size:       3554
 
NspI r_CATG'y

Cuts at:    607    1107    2014     607 
   Size:        500     907    2147

  Fragments arranged by size: 

                2147     907     500
 
PciI A'CATG_T

Cuts at:   2010    2010 
   Size:       3554
 
PmeI GTTT'AAAC

Cuts at:    553     553 
   Size:       3554
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2693    2725    2693 
   Size:         32    3522
 
PstI C_TGCA'G

Cuts at:    601    1286     601 
   Size:        685    2869
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3554
 
PvuII CAG'CTG

Cuts at:    979    1339     979 
   Size:        360    3194
 
RsrII CG'GwC_CG

Cuts at:   1749    1749 
   Size:       3554
 
SacI G_AGCT'C

Cuts at:    568     568 
   Size:       3554
 
SalI G'TCGA_C

Cuts at:    591     591 
   Size:       3554
 
SapI GCTCTTCn'nnn_

Cuts at:   1577    1787    1577 
   Size:        210    3344
 
SbfI CC_TGCA'GG

Cuts at:    601     601 
   Size:       3554
 
ScaI AGT'ACT

Cuts at:   3071    3071 
   Size:       3554
 
SfcI C'TryA_G

Cuts at:     33     506     597    1282    2275    2466      33 
   Size:        473      91     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     473     191      91
 
SfoI GGC'GCC

Cuts at:    986    1234     986 
   Size:        248    3306
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3554
 
SmaI CCC'GGG

Cuts at:    576     576 
   Size:       3554
 
SmlI C'TyrA_G

Cuts at:    709    1624    2116    2378     709 
   Size:        915     492     262    1885

  Fragments arranged by size: 

                1885     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3554
 
SphI G_CATG'C

Cuts at:    607    1107     607 
   Size:        500    3054
 
StuI AGG'CCT

Cuts at:    241     241 
   Size:       3554
 
StyI C'CwwG_G

Cuts at:    125     688    1042     125 
   Size:        563     354    2637

  Fragments arranged by size: 

                2637     563     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1137    1270    1729    1938    1137 
   Size:        133     459     209    2753

  Fragments arranged by size: 

                2753     459     209     133
 
TatI w'GTAC_w

Cuts at:   3069    3069 
   Size:       3554
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    495     508     527     540    3071    3103     495 
   Size:         13      19      13    2531      32     946

  Fragments arranged by size: 

                2531     946      32      19      13      13
 
Tth111I GACn'n_nGTC

Cuts at:    390    1351     390 
   Size:        961    2593
 
XbaI T'CTAG_A

Cuts at:    585     585 
   Size:       3554
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1049    1049 
   Size:       3554
 
XmaI C'CCGG_G

Cuts at:    574     574 
   Size:       3554
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI
     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     NspI     PciI     PmeI     PpiI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI
     BmtI    BsiWI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI
    CspCI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI
     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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