Restriction map for pF1KB6161
 (Linear) (Six-base) MAPSORT of: KIBB6161.seq  Check: 6976  from: 1  to: 1797



KIBB6161.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AccI GT'mk_AC

Cuts at:      0      63     518    1797 
   Size:         63     455    1279

  Fragments arranged by size: 

                1279     455      63
 
AclI AA'CG_TT

Cuts at:      0    1515    1797 
   Size:       1515     282
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     689    1797 
   Size:        689    1108
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1114    1797 
   Size:       1114     683
 
ApoI r'AATT_y

Cuts at:      0     362    1075    1669    1797 
   Size:        362     713     594     128

  Fragments arranged by size: 

                 713     594     362     128
 
AvaI C'yCGr_G

Cuts at:      0    1502    1797 
   Size:       1502     295
 
BanI G'GyrC_C

Cuts at:      0     202    1797 
   Size:        202    1595
 
BbeI G_GCGC'C

Cuts at:      0     206    1797 
   Size:        206    1591
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1188    1797 
   Size:       1188     609
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      53      87    1797 
   Size:         53      34    1710

  Fragments arranged by size: 

                1710      53      34
 
BclI T'GATC_A

Cuts at:      0    1365    1797 
   Size:       1365     432
 
BfrBI ATG'CAT

Cuts at:      0     113     979    1797 
   Size:        113     866     818

  Fragments arranged by size: 

                 866     818     113
 
BglI GCCn_nnn'nGGC

Cuts at:      0     200    1797 
   Size:        200    1597
 
BglII A'GATC_T

Cuts at:      0     645    1797 
   Size:        645    1152
 
BmtI G_CTAG'C

Cuts at:      0    1087    1797 
   Size:       1087     710
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1118    1797 
   Size:       1118     679
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     984    1797 
   Size:        984     813
 
BsaHI Gr'CG_yC

Cuts at:      0     203    1797 
   Size:        203    1594
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     829     859    1193    1223    1797 
   Size:        829      30     334      30     574

  Fragments arranged by size: 

                 829     574     334      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     671    1217    1797 
   Size:        671     546     580

  Fragments arranged by size: 

                 671     580     546
 
BseYI C'CCAG_C

Cuts at:      0    1131    1797 
   Size:       1131     666
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1396    1797 
   Size:       1396     401
 
BsmI GAATG_Cn'

Cuts at:      0      76    1175    1797 
   Size:         76    1099     622

  Fragments arranged by size: 

                1099     622      76
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1443    1797 
   Size:       1443     354
 
BstBI TT'CG_AA

Cuts at:      0      96    1797 
   Size:         96    1701
 
BstYI r'GATC_y

Cuts at:      0     445     553     645    1704    1797 
   Size:        445     108      92    1059      93

  Fragments arranged by size: 

                1059     445     108      93      92
 
Bsu36I CC'TnA_GG

Cuts at:      0     530    1797 
   Size:        530    1267
 
BtgI C'CryG_G

Cuts at:      0     441    1797 
   Size:        441    1356
 
ClaI AT'CG_AT

Cuts at:      0     255    1797 
   Size:        255    1542
 
DraI TTT'AAA

Cuts at:      0     359     758     993    1797 
   Size:        359     399     235     804

  Fragments arranged by size: 

                 804     399     359     235
 
EaeI y'GGCC_r

Cuts at:      0    1571    1797 
   Size:       1571     226
 
EarI CTCTTCn'nnn_

Cuts at:      0    1695    1762    1797 
   Size:       1695      67      35

  Fragments arranged by size: 

                1695      67      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1118    1797 
   Size:       1118     679
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     302    1196    1797 
   Size:        302     894     601

  Fragments arranged by size: 

                 894     601     302
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1319    1560    1797 
   Size:       1319     241     237

  Fragments arranged by size: 

                1319     241     237
 
HaeII r_GCGC'y

Cuts at:      0     206     884    1797 
   Size:        206     678     913

  Fragments arranged by size: 

                 913     678     206
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     829     861    1447    1479    1485    1517    1797 
   Size:        829      32     586      32       6      32     280

  Fragments arranged by size: 

                 829     586     280      32      32      32       6
 
HincII GTy'rAC

Cuts at:      0      14      33      64     350    1797 
   Size:         14      19      31     286    1447

  Fragments arranged by size: 

                1447     286      31      19      14
 
HpaI GTT'AAC

Cuts at:      0      14    1797 
   Size:         14    1783
 
KasI G'GCGC_C

Cuts at:      0     202    1797 
   Size:        202    1595
 
MfeI C'AATT_G

Cuts at:      0     212     593    1088    1797 
   Size:        212     381     495     709

  Fragments arranged by size: 

                 709     495     381     212
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     306     872     976    1797 
   Size:        306     566     104     821

  Fragments arranged by size: 

                 821     566     306     104
 
MscI TGG'CCA

Cuts at:      0    1573    1797 
   Size:       1573     224
 
MslI CAynn'nnrTG

Cuts at:      0     811    1797 
   Size:        811     986
 
NarI GG'CG_CC

Cuts at:      0     203    1797 
   Size:        203    1594
 
NheI G'CTAG_C

Cuts at:      0    1083    1797 
   Size:       1083     714
 
NsiI A_TGCA'T

Cuts at:      0     115     981    1797 
   Size:        115     866     816

  Fragments arranged by size: 

                 866     816     115
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     391    1797 
   Size:        391    1406
 
PfoI T'CCnGG_A

Cuts at:      0    1050    1797 
   Size:       1050     747
 
PsiI TTA'TAA

Cuts at:      0    1232    1797 
   Size:       1232     565
 
PspXI vC'TCGA_Gb

Cuts at:      0    1502    1797 
   Size:       1502     295
 
SalI G'TCGA_C

Cuts at:      0      62    1797 
   Size:         62    1735
 
SfcI C'TryA_G

Cuts at:      0      89    1399    1716    1797 
   Size:         89    1310     317      81

  Fragments arranged by size: 

                1310     317      89      81
 
SfoI GGC'GCC

Cuts at:      0     204    1797 
   Size:        204    1593
 
SmlI C'TyrA_G

Cuts at:      0     963    1502    1797 
   Size:        963     539     295

  Fragments arranged by size: 

                 963     539     295
 
StyI C'CwwG_G

Cuts at:      0     390    1797 
   Size:        390    1407
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     581    1328    1342    1797 
   Size:        581     747      14     455

  Fragments arranged by size: 

                 747     581     455      14
 
XbaI T'CTAG_A

Cuts at:      0     477    1797 
   Size:        477    1320
 
XhoI C'TCGA_G

Cuts at:      0    1502    1797 
   Size:       1502     295
 

 Enzymes that do cut:

     AccI     AclI     AlfI    AlwNI     ApoI     AvaI     BanI     BbeI
     BbsI     BcgI     BclI    BfrBI     BglI    BglII     BmtI     BpmI
    BpuEI    BsaHI    BsaXI    BseRI    BseYI     BsgI     BsmI    BspMI
    BstBI    BstYI   Bsu36I     BtgI     ClaI     DraI     EaeI     EarI
  Eco57MI    EcoNI     FalI    HaeII    Hin4I   HincII     HpaI     KasI
     MfeI     MmeI     MscI     MslI     NarI     NheI     NsiI    PflMI
     PfoI     PsiI    PspXI     SalI     SfcI     SfoI     SmlI     StyI
    TaqII     XbaI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII    BbvCI    BciVI     BlpI Bme1580I
    BmgBI     BmrI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI
  BstZ17I    BtgZI     BtsI    CspCI   DraIII     DrdI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI  HindIII
     KpnI     MluI   MspA1I     NaeI     NcoI     NdeI   NgoMIV     NotI
     NruI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XcmI     XmaI     XmnI
     ZraI
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