Restriction map for pF1KB5876
##################################
# NO CUT ENZYMES (  112 enzymes) #
##################################

AatII       Acc65I      AccI        AflII       AgeI        
Alw44I      AocI        Aor51HI     ApaLI       AscI        
AseI        AsnI        Asp718I     AspI        AviII       
AvrII       BamHI       BanI        BbeI        BbrPI       
BbvCI       BclI        BfrI        BglI        BglII       
BlnI        BsaAI       BsaBI       BsaI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspMI       BsrBI       BsrFI       BsrGI       BssHII      
Bst1107I    BstAPI      BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DrdI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FseI        FspI        HaeII       HincII      
HindII      HpaI        HphI        KasI        KpnI        
MamI        MfeI        MluI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       PvuI        PvuII       RcaI        RsrII       
SacI        SalI        SapI        SbfI        ScaI        
SfiI        SfoI        SgrAI       SmlI        SnaBI       
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   66 enzymes) #
###################################

AccII          98  CGCG                 CG^CG
AccIII       1380  TCCGGA               T^CCGGA
AclI         1326  AACGTT               AA^CGTT
AfaI         1425  GTAC                 GT^AC
AflIII        575  ACATGT               A^C[GA][CT]GT
AhdI          280  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         923  CAGTCACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI           73  GGGCCC               GGGCC^C
AspEI         280  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1196  GTGCTC               G[AT]GC[AT]^C
AvaII         967  GGTCC                G^G[AT]CC
BalI          749  TGGCCA               TGG^CCA
BbsI          366  GTCTTC               GAAGAC(2/6)
BcgI         1413  GCAGTGTGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1455  GGATAC               GTATCC(6/5)
BlpI            4  GCTGAGC              GC^T[ACGT]AGC
BpmI          695  CTCCAG               CTGGAG(16/14)
Bpu1102I        4  GCTGAGC              GC^T[ACGT]AGC
BpuAI         366  GTCTTC               GAAGAC(2/6)
BseAI        1380  TCCGGA               T^CCGGA
BsgI          620  CTGCAC               GTGCAG(16/14)
BsiHKAI      1196  GTGCTC               G[AT]GC[AT]^C
BsmBI          47  CGTCTC               CGTCTC(1/5)
BsmFI         101  GGGAC                GGGAC(10/14)
BspEI        1380  TCCGGA               T^CCGGA
BspLU11I      575  ACATGT               A^CATGT
BsrDI         121  GCAATG               GCAATG(2/0)
BssSI         625  CACGAG               CACGAG(-5/-1)
BstBI        1333  TTCGAA               TT^CGAA
BstUI          98  CGCG                 CG^CG
BstYI         987  AGATCC               [GA]^GATC[CT]
CelII           4  GCTGAGC              GC^T[ACGT]AGC
CfoI          265  GCGC                 GCG^C
DraI          547  TTTAAA               TTT^AAA
DraIII        345  CACATCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      280  GACGCCCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          792  GAAGAG               CTCTTC(1/4)
EcoT22I       658  ATGCAT               ATGCA^T
HhaI          265  GCGC                 GCG^C
HinP1I        265  GCGC                 G^CGC
HindIII      1275  AAGCTT               A^AGCTT
Ksp632I       792  GAAGAG               CTCTTC(1/4)
KspI           97  CCGCGG               CCGC^GG
MflI          987  AGATCC               [GA]^GATC[CT]
MluNI         749  TGGCCA               TGG^CCA
MroI         1380  TCCGGA               T^CCGGA
MscI          749  TGGCCA               TGG^CCA
MslI          943  CACTTGTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           98  CGCG                 CG^CG
MwoI          129  GCCGCCACCGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI          203  CCATGG               C^CATGG
NsiI          658  ATGCAT               ATGCA^T
NspV         1333  TTCGAA               TT^CGAA
PflMI         344  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I     1326  AACGTT               AA^CGTT
PstI          407  CTGCAG               CTGCA^G
RsaI         1425  GTAC                 GT^AC
SacII          97  CCGCGG               CCGC^GG
SexAI        1178  ACCAGGT              A^CC[AT]GGT
SfcI          407  CTGCAG               C^T[GA][CT]AG
SfuI         1333  TTCGAA               TT^CGAA
SpeI           13  ACTAGT               A^CTAGT
SspI          754  AATATT               AAT^ATT
Van91I        344  CCACATCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI          910  TCTAGA               T^CTAGA
XhoII         987  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   17 enzymes) #
###################################

AcyI          244  GACGCC               G[GA]^CG[CT]C
              280  GACGCC              
AlwI          988  GATCC                GGATC(4/5)
              931  GGATC               
BanII          73  GGGCCC               G[GA]GC[CT]^C
              227  GGGCTC              
BsaHI         244  GACGCC               G[GA]^CG[CT]C
              280  GACGCC              
BsaWI         972  ACCGGA               [AT]^CCGG[AT]
             1380  TCCGGA              
BseRI         797  GAGGAG               GAGGAG(10/8)
             1033  GAGGAG              
DraII          72  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               73  GGGCCCC             
DsaI           97  CCGCGG               C^C[GA][CT]GG
              203  CCATGG              
EaeI          318  CGGCCA               [CT]^GGCC[GA]
              749  TGGCCA              
EcoO109I       72  AGGGCCC              [GA]G^G[ACGT]CC[CT]
               73  GGGCCCC             
Hin1I         244  GACGCC               G[GA]^CG[CT]C
              280  GACGCC              
MspA1I         97  CCGCGG               C[AC]G^C[GT]G
              315  CAGCGG              
SfaNI         442  GATGC                GCATC(5/9)
              387  GCATC               
SmaI          223  CCCGGG               CCC^GGG
              259  CCCGGG              
SphI           42  GCATGC               GCATG^C
              660  GCATGC              
XmaCI         223  CCCGGG               C^CCGGG
              259  CCCGGG              
XmaI          223  CCCGGG               C^CCGGG
              259  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        Asp700I     
AvaI        BbvI        BcnI        BfaI        BmyI        
BsaJI       BsiYI       BslI        BsmAI       BsoBI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HinfI       HpaII       ItaI        
MaeI        MaeII       MaeIII      MboI        MboII       
MnlI        MseI        MspI        MvaI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
Sau3AI      Sau96I      ScrFI       StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XmnI        
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