Restriction map for pF1KB5876
 (Linear) (Six-base) MAPSORT of: KIBB5876.seq  Check: 3312  from: 1  to: 1485



KIBB5876.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AclI AA'CG_TT

Cuts at:      0    1327    1485 
   Size:       1327     158
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444    1175    1485 
   Size:        444     731     310

  Fragments arranged by size: 

                 731     444     310
 
AflIII A'CryG_T

Cuts at:      0     575    1485 
   Size:        575     910
 
AhdI GACnn_n'nnGTC

Cuts at:      0     285    1485 
   Size:        285    1200
 
AleI CACnn'nnGTG

Cuts at:      0     947    1485 
   Size:        947     538
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1335    1367    1485 
   Size:       1335      32     118

  Fragments arranged by size: 

                1335     118      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     928    1485 
   Size:        928     557
 
ApaI G_GGCC'C

Cuts at:      0      77    1485 
   Size:         77    1408
 
ApoI r'AATT_y

Cuts at:      0     330     417     448    1239    1485 
   Size:        330      87      31     791     246

  Fragments arranged by size: 

                 791     330     246      87      31
 
AvaI C'yCGr_G

Cuts at:      0      52     223     259    1485 
   Size:         52     171      36    1226

  Fragments arranged by size: 

                1226     171      52      36
 
BanII G_rGCy'C

Cuts at:      0      77     231    1485 
   Size:         77     154    1254

  Fragments arranged by size: 

                1254     154      77
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     359    1485 
   Size:        359    1126
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1402    1436    1485 
   Size:       1402      34      49

  Fragments arranged by size: 

                1402      49      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1449    1485 
   Size:       1449      36
 
BfrBI ATG'CAT

Cuts at:      0     660    1485 
   Size:        660     825
 
BlpI GC'TnA_GC

Cuts at:      0       5    1485 
   Size:          5    1480
 
Bme1580I G_kGCm'C

Cuts at:      0      77    1485 
   Size:         77    1408
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     207     279     467    1485 
   Size:        207      72     188    1018

  Fragments arranged by size: 

                1018     207     188      72
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     680    1485 
   Size:        680     805
 
Bpu10I CC'TnA_GC

Cuts at:      0      79    1485 
   Size:         79    1406
 
BsaHI Gr'CG_yC

Cuts at:      0     245     281    1485 
   Size:        245      36    1204

  Fragments arranged by size: 

                1204     245      36
 
BsaWI w'CCGG_w

Cuts at:      0     972    1380    1485 
   Size:        972     408     105

  Fragments arranged by size: 

                 972     408     105
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     199     229    1485 
   Size:        199      30    1256

  Fragments arranged by size: 

                1256     199      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     812    1048    1485 
   Size:        812     236     437

  Fragments arranged by size: 

                 812     437     236
 
BseYI C'CCAG_C

Cuts at:      0     313    1485 
   Size:        313    1172
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     605    1485 
   Size:        605     880
 
BsiHKAI G_wGCw'C

Cuts at:      0    1200    1485 
   Size:       1200     285
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      53    1485 
   Size:         53    1432
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     231    1200    1485 
   Size:         77     154     969     285

  Fragments arranged by size: 

                 969     285     154      77
 
BspEI T'CCGG_A

Cuts at:      0    1380    1485 
   Size:       1380     105
 
BsrDI GCAATG_nn'

Cuts at:      0     128    1485 
   Size:        128    1357
 
BssSI C'ACGA_G

Cuts at:      0     625    1485 
   Size:        625     860
 
BstBI TT'CG_AA

Cuts at:      0    1334    1485 
   Size:       1334     151
 
BstYI r'GATC_y

Cuts at:      0     987    1485 
   Size:        987     498
 
BtgI C'CryG_G

Cuts at:      0      97     203    1485 
   Size:         97     106    1282

  Fragments arranged by size: 

                1282     106      97
 
BtsI GCAGTG_nn'

Cuts at:      0      86    1200    1420    1485 
   Size:         86    1114     220      65

  Fragments arranged by size: 

                1114     220      86      65
 
DraI TTT'AAA

Cuts at:      0     549    1485 
   Size:        549     936
 
DraIII CAC_nnn'GTG

Cuts at:      0     350    1485 
   Size:        350    1135
 
EaeI y'GGCC_r

Cuts at:      0     318     749    1485 
   Size:        318     431     736

  Fragments arranged by size: 

                 736     431     318
 
EarI CTCTTCn'nnn_

Cuts at:      0     787    1485 
   Size:        787     698
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     221    1485 
   Size:        221    1264
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     680    1175    1485 
   Size:        444     236     495     310

  Fragments arranged by size: 

                 495     444     310     236
 
EcoO109I rG'GnC_Cy

Cuts at:      0      73      74    1485 
   Size:         73       1    1411

  Fragments arranged by size: 

                1411      73       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1293    1485 
   Size:       1293     192
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     199     231     271     303     858     890    1006    1038 
   Size:        199      32      40      32     555      32     116      32

Cuts at:   1038    1197    1229    1485 
   Size:        159      32     256

  Fragments arranged by size: 

                 555     256     199     159     116      40      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0    1275    1485 
   Size:       1275     210
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     766    1485 
   Size:        766     719
 
MscI TGG'CCA

Cuts at:      0     751    1485 
   Size:        751     734
 
MslI CAynn'nnrTG

Cuts at:      0     947    1485 
   Size:        947     538
 
MspA1I CmG'CkG

Cuts at:      0      99     317    1485 
   Size:         99     218    1168

  Fragments arranged by size: 

                1168     218      99
 
NcoI C'CATG_G

Cuts at:      0     203    1485 
   Size:        203    1282
 
NsiI A_TGCA'T

Cuts at:      0     662    1485 
   Size:        662     823
 
NspI r_CATG'y

Cuts at:      0      46     579     664    1485 
   Size:         46     533      85     821

  Fragments arranged by size: 

                 821     533      85      46
 
PciI A'CATG_T

Cuts at:      0     575    1485 
   Size:        575     910
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     350    1485 
   Size:        350    1135
 
PspOMI G'GGCC_C

Cuts at:      0      73    1485 
   Size:         73    1412
 
PstI C_TGCA'G

Cuts at:      0     411    1485 
   Size:        411    1074
 
SacII CC_GC'GG

Cuts at:      0     100    1485 
   Size:        100    1385
 
SexAI A'CCwGG_T

Cuts at:      0    1178    1485 
   Size:       1178     307
 
SfcI C'TryA_G

Cuts at:      0     407    1485 
   Size:        407    1078
 
SmaI CCC'GGG

Cuts at:      0     225     261    1485 
   Size:        225      36    1224

  Fragments arranged by size: 

                1224     225      36
 
SpeI A'CTAG_T

Cuts at:      0      13    1485 
   Size:         13    1472
 
SphI G_CATG'C

Cuts at:      0      46     664    1485 
   Size:         46     618     821

  Fragments arranged by size: 

                 821     618      46
 
SspI AAT'ATT

Cuts at:      0     756    1485 
   Size:        756     729
 
StyI C'CwwG_G

Cuts at:      0     203     325    1061    1485 
   Size:        203     122     736     424

  Fragments arranged by size: 

                 736     424     203     122
 
XbaI T'CTAG_A

Cuts at:      0     910    1485 
   Size:        910     575
 
XmaI C'CCGG_G

Cuts at:      0     223     259    1485 
   Size:        223      36    1226

  Fragments arranged by size: 

                1226     223      36
 
XmnI GAAnn'nnTTC

Cuts at:      0     397    1242    1370    1485 
   Size:        397     845     128     115

  Fragments arranged by size: 

                 845     397     128     115
 

 Enzymes that do cut:

     AclI     AcuI   AflIII     AhdI     AleI     AloI    AlwNI     ApaI
     ApoI     AvaI    BanII     BbsI     BcgI    BciVI    BfrBI     BlpI
 Bme1580I     BmrI     BpmI   Bpu10I    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BsrDI    BssSI
    BstBI    BstYI     BtgI     BtsI     DraI   DraIII     EaeI     EarI
     EciI  Eco57MI EcoO109I     FalI    Hin4I  HindIII     MmeI     MscI
     MslI   MspA1I     NcoI     NsiI     NspI     PciI    PflMI   PspOMI
     PstI    SacII    SexAI     SfcI     SmaI     SpeI     SphI     SspI
     StyI     XbaI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AlfI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BclI     BglI    BglII    BmgBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BspHI
    BspMI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DrdI     EagI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MfeI     MluI     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI
    PvuII    RsrII     SacI     SalI    SanDI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SrfI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XcmI     XhoI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com