Restriction map for pF1KB7667
##################################
# NO CUT ENZYMES (  150 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      Alw44I      
AlwNI       ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BciVI       BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaI        BseAI       BseRI       BsiEI       
BsiWI       BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstUI       BstXI       BstYI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EagI        EarI        
EclXI       Eco52I      EcoNI       EcoO109I    EcoRI       
EcoRV       EcoT22I     FauI        FseI        FspI        
HgaI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MamI        MfeI        MflI        MluI        MroI        
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcsI          514  AAATTC               [GA]^AATT[CT]
AhdI          305  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI          465  GGATC                GGATC(4/5)
AocI          307  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       284  AGCGCT               AGC^GCT
ApoI          514  AAATTC               [GA]^AATT[CT]
Asp700I       831  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         305  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI          338  TGGCCA               TGG^CCA
BanI           66  GGTGCC               G^G[CT][GA]CC
BanII         147  GAGCCC               G[GA]GC[CT]^C
BclI          407  TGATCA               T^GATCA
BcnI           51  CCGGG                CC^[GC]GG
BfaI          922  CTAG                 C^TAG
BsgI          744  CTGCAC               GTGCAG(16/14)
BsiYI         865  CCTGGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          865  CCTGGTGATGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1407I      557  TGTACA               T^GTACA
BspMI         813  GCAGGT               ACCTGC(4/8)
BsrDI         561  CATTGC               GCAATG(2/0)
BsrGI         557  TGTACA               T^GTACA
BstEII        694  GGTTACC              G^GT[ACGT]ACC
BstF5I        954  GGATG                GGATG(2/0)
BstPI         694  GGTTACC              G^GT[ACGT]ACC
Bsu36I        307  CCTGAGG              CC^T[ACGT]AGG
CfoI          285  GCGC                 GCG^C
EaeI          338  TGGCCA               [CT]^GGCC[GA]
Eam1105I      305  GACCTGAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      284  AGCGCT               AGC^GCT
Eco81I        307  CCTGAGG              CC^T[ACGT]AGG
EcoO65I       694  GGTTACC              G^GT[ACGT]ACC
FbaI          407  TGATCA               T^GATCA
FokI          954  GGATG                GGATG(9/13)
HaeII         284  AGCGCT               [GA]GCGC^[CT]
HhaI          285  GCGC                 GCG^C
HinP1I        285  GCGC                 G^CGC
MaeI          922  CTAG                 C^TAG
MaeII         116  ACGT                 A^CGT
MluNI         338  TGGCCA               TGG^CCA
MscI          338  TGGCCA               TGG^CCA
MspA1I        420  CCGCTG               C[AC]G^C[GT]G
MwoI          425  GCCACCACTGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI           51  CCGGG                CC^[GC]GG
NheI          921  GCTAGC               G^CTAGC
NspI          662  GCATGT               [GA]CATG^[CT]
PleI          904  GAGTC                GAGTC(4/5)
PshAI         394  GACCGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfaNI         324  GATGC                GCATC(5/9)
SspBI         557  TGTACA               T^GTACA
StuI          737  AGGCCT               AGG^CCT
XcmI          810  CCAGCAGGTGTTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          831  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AgeI          351  ACCGGT               A^CCGGT
              395  ACCGGT              
AspHI          73  GTGCTC               G[AT]GC[AT]^C
               94  GTGCTC              
BbsI          943  GAAGAC               GAAGAC(2/6)
              574  GTCTTC              
BpmI          749  CTCCAG               CTGGAG(16/14)
              597  CTGGAG              
BpuAI         943  GAAGAC               GAAGAC(2/6)
              574  GTCTTC              
BsaWI         351  ACCGGT               [AT]^CCGG[AT]
              395  ACCGGT              
BsiHKAI        73  GTGCTC               G[AT]GC[AT]^C
               94  GTGCTC              
BsrFI         351  ACCGGT               [GA]^CCGG[CT]
              395  ACCGGT              
Cac8I         658  GCCTGC               GC[ACGT]^[ACGT]GC
              921  GCTAGC              
Cfr10I        351  ACCGGT               [GA]^CCGG[CT]
              395  ACCGGT              
DpnII         408  GATC                 ^GATC
              466  GATC                
EcoT14I       478  CCTTGG               C^C[AT][AT]GG
              789  CCTTGG              
HphI          135  GGTGA                GGTGA(8/7)
              868  GGTGA               
MboI          408  GATC                 ^GATC
              466  GATC                
MslI          189  CATCTTTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              747  CACTCCAGTG          
NdeII         408  GATC                 ^GATC
              466  GATC                
NlaIV          66  GGTGCC               GG[ACGT]^[ACGT]CC
              856  GGTTCC              
PinAI         351  ACCGGT               A^CCGGT
              395  ACCGGT              
Sau3AI        408  GATC                 ^GATC
              466  GATC                
StyI          478  CCTTGG               C^C[AT][AT]GG
              789  CCTTGG              
TfiI          386  GATTC                G^A[AT]TC
              684  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   35 enzymes) #
#########################################

AciI        AfaI        AluI        AvaII       BbvI        
BmyI        BsaJI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DdeI        EcoRII      Fnu4HI      
HaeIII      HapII       HinfI       HpaII       ItaI        
MaeIII      MboII       MnlI        MseI        MspI        
MvaI        NlaIII      RsaI        Sau96I      ScrFI       
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
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