Restriction map for pF1KB7627
##################################
# NO CUT ENZYMES (  167 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AlwI        AlwNI       Aor51HI     
ApaI        ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstYI       BstZ17I     CelII       CfoI        
Cfr10I      ClaI        CpoI        DpnII       DraI        
DraII       DraIII      DrdI        EaeI        EagI        
EclXI       Eco47III    Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HapII       
HgaI        HhaI        Hin1I       HinP1I      HindIII     
HpaI        HpaII       KasI        KpnI        KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NdeI        NdeII       NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        Sau3AI      SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SphI        
SspBI       SspI        SwaI        Tsp509I     Tth111I     
Van91I      XbaI        XcmI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AhdI           38  GACGGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        318  GTGCAC               G^TGCAC
AocI          400  CCTCAGG              CC^T[ACGT]AGG
ApaLI         318  GTGCAC               G^TGCAC
AspEI          38  GACGGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          652  CTCGAG               C^[CT]CG[GA]G
BseRI         134  CTCCTC               GAGGAG(10/8)
BsmI          757  GAATGC               GAATGC(1/-1)
BsoBI         652  CTCGAG               C^[CT]CG[GA]G
BsrDI         505  CATTGC               GCAATG(2/0)
BstF5I        312  CATCC                GGATG(2/0)
BstXI          54  CCAACGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        400  CCTCAGG              CC^T[ACGT]AGG
DsaI          704  CCATGG               C^C[GA][CT]GG
Eam1105I       38  GACGGTGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        400  CCTCAGG              CC^T[ACGT]AGG
FokI          312  CATCC                GGATG(9/13)
HincII        217  GTCAAC               GT[CT]^[GA]AC
HindII        217  GTCAAC               GT[CT]^[GA]AC
MaeIII         46  GTCAC                ^GT[ACGT]AC
MseI          641  TTAA                 T^TAA
MspA1I        153  CAGCTG               C[AC]G^C[GT]G
NcoI          704  CCATGG               C^CATGG
NlaIII        705  CATG                 CATG^
PaeR7I        652  CTCGAG               C^TCGAG
PvuII         153  CAGCTG               CAG^CTG
SacI          859  GAGCTC               GAGCT^C
SapI          468  GCTCTTC              GCTCTTC(1/4)
SbfI          294  CCTGCAGG             CCTGCA^GG
ScaI           20  AGTACT               AGT^ACT
SmlI          652  CTCGAG               C^T[CT][GA]AG
SpeI          673  ACTAGT               A^CTAGT
Sse8387I      294  CCTGCAGG             CCTGCA^GG
StuI          291  AGGCCT               AGG^CCT
TfiI          559  GAATC                G^A[AT]TC
Tru9I         641  TTAA                 T^TAA
Tsp45I         46  GTCAC                ^GT[GC]AC
XhoI          652  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

AfaI           21  GTAC                 GT^AC
              144  GTAC                
AspHI         318  GTGCAC               G[AT]GC[AT]^C
              859  GAGCTC              
BanII         466  GGGCTC               G[GA]GC[CT]^C
              859  GAGCTC              
BfaI          439  CTAG                 C^TAG
              674  CTAG                
BsaI          589  GAGACC               GGTCTC(1/5)
              814  GAGACC              
BsiHKAI       318  GTGCAC               G[AT]GC[AT]^C
              859  GAGCTC              
BstAPI        122  GCACAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              311  GCATCCCGTGC         
EarI          469  CTCTTC               CTCTTC(1/4)
              565  CTCTTC              
Ksp632I       469  CTCTTC               CTCTTC(1/4)
              565  CTCTTC              
MaeI          439  CTAG                 C^TAG
              674  CTAG                
PleI          601  GAGTC                GAGTC(4/5)
              694  GAGTC               
RsaI           21  GTAC                 GT^AC
              144  GTAC                


#########################################
# 3 OR MORE CUT ENZYMES (   41 enzymes) #
#########################################

AluI        AvaII       BbvI        BmyI        BpmI        
BsaJI       BsgI        BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstNI       Cac8I       Cfr13I      DdeI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      HinfI       HphI        
ItaI        MboII       MnlI        MvaI        MwoI        
NlaIV       PstI        Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TseI        TspRI       
TthHB8I     
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