Restriction map for pF1KB7627
 (Circular) (Six-base) MAPSORT of: pF1KB7627.seq  Check: 2756  from: 1  to: 4004



pF1KB7627  4004 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    166     166 
   Size:       4004
 
AccI GT'mk_AC

Cuts at:   1042    1042 
   Size:       4004
 
Acc65I G'GTAC_C

Cuts at:   1020    1020 
   Size:       4004
 
AclI AA'CG_TT

Cuts at:   3844    3844 
   Size:       4004
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1829    2261    3008    3276    1829 
   Size:        432     747     268    2557

  Fragments arranged by size: 

                2557     747     432     268
 
AflIII A'CryG_T

Cuts at:   1356    2460    3437    1356 
   Size:       1104     977    1923

  Fragments arranged by size: 

                1923    1104     977
 
AgeI A'CCGG_T

Cuts at:   2392    2392 
   Size:       4004
 
AhdI GACnn_n'nnGTC

Cuts at:    151     151 
   Size:       4004
 
AlwNI CAG_nnn'CTG

Cuts at:   1309    2876    1309 
   Size:       1567    2437
 
ApaLI G'TGCA_C

Cuts at:    426    2774     426 
   Size:       2348    1656
 
ApoI r'AATT_y

Cuts at:   1008    3357    3613    1008 
   Size:       2349     256    1399

  Fragments arranged by size: 

                2349    1399     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4004
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4004
 
AvaI C'yCGr_G

Cuts at:    760    1024     760 
   Size:        264    3740
 
BamHI G'GATC_C

Cuts at:   1029    1029 
   Size:       4004
 
BanI G'GyrC_C

Cuts at:   1020    1434    1682    1717    1020 
   Size:        414     248      35    3307

  Fragments arranged by size: 

                3307     414     248      35
 
BanII G_rGCy'C

Cuts at:    578     971    1018    2048     578 
   Size:        393      47    1030    2534

  Fragments arranged by size: 

                2534    1030     393      47
 
BbeI G_GCGC'C

Cuts at:   1438    1686    1438 
   Size:        248    3756
 
BciVI GTATCCnnnnn_n'

Cuts at:   1902    2663    4004    1902 
   Size:        761    1341    1902

  Fragments arranged by size: 

                1902    1341     761
 
BclI T'GATC_A

Cuts at:   1524    1524 
   Size:       4004
 
BglII A'GATC_T

Cuts at:   1519    3556    1519 
   Size:       2037    1967
 
BlpI GC'TnA_GC

Cuts at:   1116    1116 
   Size:       4004
 
Bme1580I G_kGCm'C

Cuts at:    430     506     980    1629    1722    2778     430 
   Size:         76     474     649      93    1056    1656

  Fragments arranged by size: 

                1656    1056     649     474      93      76
 
BmrI ACTGGGnnnn_n'

Cuts at:    141     855    1216    1297    1632    3776     141 
   Size:        714     361      81     335    2144     369

  Fragments arranged by size: 

                2144     714     369     361     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    855    2371     855 
   Size:       1516    2488
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    935     974     985     935 
   Size:         39      11    3954

  Fragments arranged by size: 

                3954      39      11
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1180    2059    2551    2849    1180 
   Size:        879     492     298    2335

  Fragments arranged by size: 

                2335     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    691     916    3708     691 
   Size:        225    2792     987

  Fragments arranged by size: 

                2792     987     225
 
BsaAI yAC'GTr

Cuts at:   1357    1357 
   Size:       4004
 
BsaBI GATnn'nnATC

Cuts at:      6    1518    3555       6 
   Size:       1512    2037     455

  Fragments arranged by size: 

                2037    1512     455
 
BsaHI Gr'CG_yC

Cuts at:   1435    1683    2385    3881    1435 
   Size:        248     702    1496    1558

  Fragments arranged by size: 

                1558    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1193    1714    2392    2666    2813    3224    1193 
   Size:        521     678     274     147     411    1973

  Fragments arranged by size: 

                1973     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    233     233 
   Size:       4004
 
BseYI C'CCAG_C

Cuts at:    263     688     740     991    1429    2187    2764    3260     263 
   Size:        425      52     251     438     758     577     496    1007

  Fragments arranged by size: 

                1007     758     577     496     438     425     251      52
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    213     315     807     213 
   Size:        102     492    3410

  Fragments arranged by size: 

                3410     492     102
 
BsiEI CG_ry'CG

Cuts at:    104    1546    1592    2800     104 
   Size:       1442      46    1208    1308

  Fragments arranged by size: 

                1442    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    430     971    1018    1796    1986    2778     430 
   Size:        541      47     778     190     792    1656

  Fragments arranged by size: 

                1656     792     778     541     190      47
 
BsmI GAATG_Cn'

Cuts at:    871     871 
   Size:       4004
 
Bsp1286I G_dGCh'C

Cuts at:    430     506     578     971     980    1018    1629    1722    1796 
   Size:         76      72     393       9      38     611      93      74

Cuts at:   1796    1986    2048    2778     430 
   Size:        190      62     730    1656

  Fragments arranged by size: 

                1656     730     611     393     190      93      76      74
                  72      62      38       9
 
BspEI T'CCGG_A

Cuts at:   3224    3224 
   Size:       4004
 
BspHI T'CATG_A

Cuts at:   3180    3180 
   Size:       4004
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    166     239     890    1054    1268    1570    1951     166 
   Size:         73     651     164     214     302     381    2219

  Fragments arranged by size: 

                2219     651     381     302     214     164      73
 
BsrBI CCG'CTC

Cuts at:     50    2296    2350    3834    3999      50 
   Size:       2246      54    1484     165      55

  Fragments arranged by size: 

                2246    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    612    1916     612 
   Size:       1304    2700
 
BsrFI r'CCGG_y

Cuts at:   2002    2183    2392    2002 
   Size:        181     209    3614

  Fragments arranged by size: 

                3614     209     181
 
BssSI C'ACGA_G

Cuts at:   2275    2633    2275 
   Size:        358    3646
 
BstAPI GCAn_nnn'nTGC

Cuts at:    236     425    1381     236 
   Size:        189     956    2859

  Fragments arranged by size: 

                2859     956     189
 
BstBI TT'CG_AA

Cuts at:   2365    2365 
   Size:       4004
 
BstXI CCAn_nnnn'nTGG

Cuts at:    169     169 
   Size:       4004
 
BstYI r'GATC_y

Cuts at:      1    1029    1496    1519    1854    2100    3112    3198    3210 
   Size:       1028     467      23     335     246    1012      86      12

Cuts at:   3210    3556       1 
   Size:        346     449

  Fragments arranged by size: 

                1028    1012     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    509     509 
   Size:       4004
 
BtgI C'CryG_G

Cuts at:    107     812     107 
   Size:        705    3299
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1954    2135    2298    1954 
   Size:        181     163    3660

  Fragments arranged by size: 

                3660     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1247    1247 
   Size:       4004
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    180     215     507     542     180 
   Size:         35     292      35    3642

  Fragments arranged by size: 

                3642     292      35      35
 
DraI TTT'AAA

Cuts at:   1003    1003 
   Size:       4004
 
DrdI GACnn_nn'nnGTC

Cuts at:   1710    2568    1710 
   Size:        858    3146
 
EaeI y'GGCC_r

Cuts at:   1589    1763    2154    2181    3526    1589 
   Size:        174     391      27    1345    2067

  Fragments arranged by size: 

                2067    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1589    1589 
   Size:       4004
 
EarI CTCTTCn'nnn_

Cuts at:    583     679    2027    2237     583 
   Size:         96    1348     210    2350

  Fragments arranged by size: 

                2350    1348     210      96
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2212    2522    2668    3814    2212 
   Size:        310     146    1146    2402

  Fragments arranged by size: 

                2402    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    969    1016     969 
   Size:         47    3957
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    935     974     985    1829    2261    3008    3276     935 
   Size:         39      11     844     432     747     268    1663

  Fragments arranged by size: 

                1663     844     747     432     268      39      11
 
EcoO109I rG'GnC_Cy

Cuts at:   1143    1143 
   Size:       4004
 
EcoRI G'AATT_C

Cuts at:   1008    1008 
   Size:       4004
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    618    2023     618 
   Size:       1405    2599
 
FspI TGC'GCA

Cuts at:   1785    1785 
   Size:       4004
 
HaeII r_GCGC'y

Cuts at:   1438    1686    2708    3687    1438 
   Size:        248    1022     979    1755

  Fragments arranged by size: 

                1755    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    137     169     760     792    1846    1876    1878    1908     137 
   Size:         32     591      32    1054      30       2      30    2233

  Fragments arranged by size: 

                2233    1054     591      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    327    1043     327 
   Size:        716    3288
 
KasI G'GCGC_C

Cuts at:   1434    1682    1434 
   Size:        248    3756
 
KpnI G_GTAC'C

Cuts at:   1024    1024 
   Size:       4004
 
MluI A'CGCG_T

Cuts at:   3437    3437 
   Size:       4004
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2675    2859    2675 
   Size:        184    3820
 
MscI TGG'CCA

Cuts at:   1765    1765 
   Size:       4004
 
MslI CAynn'nnrTG

Cuts at:   1993    2120    1993 
   Size:        127    3877
 
MspA1I CmG'CkG

Cuts at:    263    1115    1429    1789    2802    3047     263 
   Size:        852     314     360    1013     245    1220

  Fragments arranged by size: 

                1220    1013     852     360     314     245
 
NaeI GCC'GGC

Cuts at:   2185    2185 
   Size:       4004
 
NarI GG'CG_CC

Cuts at:   1435    1683    1435 
   Size:        248    3756
 
NcoI C'CATG_G

Cuts at:    107     812     107 
   Size:        705    3299
 
NgoMIV G'CCGG_C

Cuts at:   2183    2183 
   Size:       4004
 
NspI r_CATG'y

Cuts at:   1057    1557    2464    1057 
   Size:        500     907    2597

  Fragments arranged by size: 

                2597     907     500
 
PciI A'CATG_T

Cuts at:   2460    2460 
   Size:       4004
 
PfoI T'CCnGG_A

Cuts at:    174     174 
   Size:       4004
 
PmeI GTTT'AAAC

Cuts at:   1003    1003 
   Size:       4004
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3143    3175    3143 
   Size:         32    3972
 
PspXI vC'TCGA_Gb

Cuts at:    760     760 
   Size:       4004
 
PstI C_TGCA'G

Cuts at:    407     638     775    1051    1736     407 
   Size:        231     137     276     685    2675

  Fragments arranged by size: 

                2675     685     276     231     137
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4004
 
PvuII CAG'CTG

Cuts at:    263    1429    1789     263 
   Size:       1166     360    2478

  Fragments arranged by size: 

                2478    1166     360
 
RsrII CG'GwC_CG

Cuts at:   2199    2199 
   Size:       4004
 
SacI G_AGCT'C

Cuts at:    971    1018     971 
   Size:         47    3957
 
SalI G'TCGA_C

Cuts at:   1041    1041 
   Size:       4004
 
SapI GCTCTTCn'nnn_

Cuts at:    583    2027    2237     583 
   Size:       1444     210    2350

  Fragments arranged by size: 

                2350    1444     210
 
SbfI CC_TGCA'GG

Cuts at:    407    1051     407 
   Size:        644    3360
 
ScaI AGT'ACT

Cuts at:    130    3521     130 
   Size:       3391     613
 
SfcI C'TryA_G

Cuts at:     33     403     634     771    1047    1732    2725    2916      33 
   Size:        370     231     137     276     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     370     276     231     191     137
            
 
SfoI GGC'GCC

Cuts at:   1436    1684    1436 
   Size:        248    3756
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4004
 
SmaI CCC'GGG

Cuts at:   1026    1026 
   Size:       4004
 
SmlI C'TyrA_G

Cuts at:    760    1159    2074    2566    2828     760 
   Size:        399     915     492     262    1936

  Fragments arranged by size: 

                1936     915     492     399     262
 
SpeI A'CTAG_T

Cuts at:     67     781      67 
   Size:        714    3290
 
SphI G_CATG'C

Cuts at:   1057    1557    1057 
   Size:        500    3504
 
StuI AGG'CCT

Cuts at:    401     401 
   Size:       4004
 
StyI C'CwwG_G

Cuts at:    107     191     812     926    1138    1492     107 
   Size:         84     621     114     212     354    2619

  Fragments arranged by size: 

                2619     621     354     212     114      84
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    510     702    1587    1720    2179    2388     510 
   Size:        192     885     133     459     209    2126

  Fragments arranged by size: 

                2126     885     459     209     192     133
 
TatI w'GTAC_w

Cuts at:    128    3519     128 
   Size:       3391     613
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    400     432    3521    3553     400 
   Size:         32    3089      32     851

  Fragments arranged by size: 

                3089     851      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1801    1801 
   Size:       4004
 
XbaI T'CTAG_A

Cuts at:   1035    1035 
   Size:       4004
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1499    1499 
   Size:       4004
 
XhoI C'TCGA_G

Cuts at:    760     760 
   Size:       4004
 
XmaI C'CCGG_G

Cuts at:   1024    1024 
   Size:       4004
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BsaXI    BsiWI    BsmBI    BsrGI   BssHII   BstEII
  BstZ17I     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PmlI    PpuMI    PshAI     PsiI   PspOMI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI
     SwaI     XmnI     ZraI
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