Restriction map for pF1KE0706
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvrII       BamHI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfrI        
BglII       BlnI        BpuAI       BsaBI       BsaI        
BsaWI       BseAI       BsgI        BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     ClaI        CpoI        
DsaI        Eam1105I    Eco47III    EcoO65I     EcoRV       
EcoT14I     FbaI        FseI        HindIII     HpaI        
KpnI        KspI        MamI        MflI        MluI        
MroI        MvnI        NcoI        NdeI        NheI        
NotI        NruI        NspV        PacI        PflFI       
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        PvuII       RsaI        RsrII       SacII       
SalI        ScaI        SexAI       SfiI        SfuI        
SnaBI       SpeI        SphI        SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tth111I     
XbaI        XcmI        XhoII       


###################################
# ONE CUT ENZYMES (   55 enzymes) #
###################################

AocI          841  CCTCAGG              CC^T[ACGT]AGG
ApaI         1146  GGGCCC               GGGCC^C
Asp700I        30  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII          77  TGCGCA               TGC^GCA
BalI          203  TGGCCA               TGG^CCA
BbeI           13  GGCGCC               GGCGC^C
BbrPI         672  CACGTG               CAC^GTG
BfaI          223  CTAG                 C^TAG
BglI          669  GCCCACGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         672  CACGTG               [CT]AC^GT[GA]
BsiEI         286  CGGCCG               CG[GA][CT]^CG
BsmBI         980  CGTCTC               CGTCTC(1/5)
BspMI        1048  GCAGGT               ACCTGC(4/8)
Bsu36I        841  CCTCAGG              CC^T[ACGT]AGG
DraI          172  TTTAAA               TTT^AAA
DraIII       1189  CACAACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          502  GACCAGGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          286  CGGCCG               C^GGCCG
EclXI         286  CGGCCG               C^GGCCG
Eco52I        286  CGGCCG               C^GGCCG
Eco81I        841  CCTCAGG              CC^T[ACGT]AGG
EcoNI         808  CCTCCTGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         356  GAATTC               G^AATTC
EcoT22I       517  ATGCAT               ATGCA^T
FspI           77  TGCGCA               TGC^GCA
HincII        511  GTCAAC               GT[CT]^[GA]AC
HindII        511  GTCAAC               GT[CT]^[GA]AC
HinfI          72  GACTC                G^A[ACGT]TC
KasI           13  GGCGCC               G^GCGCC
MaeI          223  CTAG                 C^TAG
MfeI          366  CAATTG               C^AATTG
MluNI         203  TGGCCA               TGG^CCA
MscI          203  TGGCCA               TGG^CCA
MslI          364  CACAATTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I       1164  CAGCGG               C[AC]G^C[GT]G
MunI          366  CAATTG               C^AATTG
NarI           13  GGCGCC               GG^CGCC
NsiI          517  ATGCAT               ATGCA^T
NspI          515  ACATGC               [GA]CATG^[CT]
PaeR7I        149  CTCGAG               C^TCGAG
PleI           72  GACTC                GAGTC(4/5)
PmaCI         672  CACGTG               CAC^GTG
PmlI          672  CACGTG               CAC^GTG
PstI          749  CTGCAG               CTGCA^G
SacI          382  GAGCTC               GAGCT^C
SapI           33  GCTCTTC              GCTCTTC(1/4)
SbfI          748  CCTGCAGG             CCTGCA^GG
SfaNI         267  GATGC                GCATC(5/9)
SfcI          749  CTGCAG               C^T[GA][CT]AG
SfoI           13  GGCGCC               GGC^GCC
SgrAI        1155  CGCCGGCG             C[GA]^CCGG[CT]G
Sse8387I      748  CCTGCAGG             CCTGCA^GG
Tsp45I        415  GTCAC                ^GT[GC]AC
XhoI          149  CTCGAG               C^TCGAG
XmnI           30  GAAGCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   38 enzymes) #
###################################

AcsI          169  AAATTT               [GA]^AATT[CT]
              356  GAATTC              
AcyI           13  GGCGCC               G[GA]^CG[CT]C
              439  GACGCC              
ApoI          169  AAATTT               [GA]^AATT[CT]
              356  GAATTC              
AspHI          57  GAGCAC               G[AT]GC[AT]^C
              382  GAGCTC              
BlpI          562  GCTGAGC              GC^T[ACGT]AGC
             1018  GCTCAGC             
BpmI           54  CTGGAG               CTGGAG(16/14)
              812  CTGGAG              
Bpu1102I      562  GCTGAGC              GC^T[ACGT]AGC
             1018  GCTCAGC             
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
              439  GACGCC              
BseRI         929  CTCCTC               GAGGAG(10/8)
              758  GAGGAG              
BsiHKAI        57  GAGCAC               G[AT]GC[AT]^C
              382  GAGCTC              
BspHI         122  TCATGA               T^CATGA
              428  TCATGA              
BsrFI         711  GCCGGC               [GA]^CCGG[CT]
             1156  GCCGGC              
BstF5I        266  GGATG                GGATG(2/0)
              298  GGATG               
CelII         562  GCTGAGC              GC^T[ACGT]AGC
             1018  GCTCAGC             
Cfr10I        711  GCCGGC               [GA]^CCGG[CT]
             1156  GCCGGC              
EarI           34  CTCTTC               CTCTTC(1/4)
              755  GAAGAG              
FokI          266  GGATG                GGATG(9/13)
              298  GGATG               
HaeII          13  GGCGCC               [GA]GCGC^[CT]
              588  AGCGCC              
HgaI          439  GACGC                GACGC(5/10)
             1217  GACGC               
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
              439  GACGCC              
HphI          373  GGTGA                GGTGA(8/7)
              785  TCACC               
Ksp632I        34  CTCTTC               CTCTTC(1/4)
              755  GAAGAG              
MaeII         673  ACGT                 A^CGT
             1193  ACGT                
MaeIII        415  GTCAC                ^GT[ACGT]AC
             1052  GTTAC               
MboII         755  GAAGA                GAAGA(8/7)
               35  TCTTC               
MseI          173  TTAA                 T^TAA
              554  TTAA                
NaeI          711  GCCGGC               GCC^GGC
             1156  GCCGGC              
NgoMIV        711  GCCGGC               G^CCGGC
             1156  GCCGGC              
PflMI         608  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              644  CCAATGGCTGG         
PpuMI         309  AGGACCC              [GA]G^G[AT]CC[CT]
             1122  GGGTCCC             
RcaI          122  TCATGA               T^CATGA
              428  TCATGA              
SmaI          260  CCCGGG               CCC^GGG
              946  CCCGGG              
SmlI          149  CTCGAG               C^T[CT][GA]AG
             1065  CTTGAG              
Tru9I         173  TTAA                 T^TAA
              554  TTAA                
TspRI         497  CAGTG                CA[GC]TG[ACGT][ACGT]^
              613  CAGTG               
Van91I        608  CCAGCCAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              644  CCAATGGCTGG         
XmaCI         260  CCCGGG               C^CCGGG
              946  CCCGGG              
XmaI          260  CCCGGG               C^CCGGG
              946  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   52 enzymes) #
#########################################

AciI        AluI        AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrI        BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HpaII       
ItaI        MboI        MnlI        MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       TaqI        TseI        
Tsp509I     TthHB8I     
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