Restriction map for pF1KE0706
 (Linear) (Six-base) MAPSORT of: KIEE0706.seq  Check: 4916  from: 1  to: 1221



KIEE0706.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:27  ..

AleI CACnn'nnGTG

Cuts at:      0     368    1221 
   Size:        368     853
 
ApaI G_GGCC'C

Cuts at:      0    1150    1221 
   Size:       1150      71
 
ApoI r'AATT_y

Cuts at:      0     169     356    1221 
   Size:        169     187     865

  Fragments arranged by size: 

                 865     187     169
 
AvaI C'yCGr_G

Cuts at:      0       9     149     260     458     569     946    1221 
   Size:          9     140     111     198     111     377     275

  Fragments arranged by size: 

                 377     275     198     140     111     111       9
 
BanI G'GyrC_C

Cuts at:      0      13     604     708     853    1221 
   Size:         13     591     104     145     368

  Fragments arranged by size: 

                 591     368     145     104      13
 
BanII G_rGCy'C

Cuts at:      0     386     765    1150    1221 
   Size:        386     379     385      71

  Fragments arranged by size: 

                 386     385     379      71
 
BbeI G_GCGC'C

Cuts at:      0      17    1221 
   Size:         17    1204
 
BfrBI ATG'CAT

Cuts at:      0     519    1221 
   Size:        519     702
 
BglI GCCn_nnn'nGGC

Cuts at:      0     675    1221 
   Size:        675     546
 
BlpI GC'TnA_GC

Cuts at:      0     563    1019    1221 
   Size:        563     456     202

  Fragments arranged by size: 

                 563     456     202
 
Bme1580I G_kGCm'C

Cuts at:      0     635     858    1150    1221 
   Size:        635     223     292      71

  Fragments arranged by size: 

                 635     292     223      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     833    1221 
   Size:         75     758     388

  Fragments arranged by size: 

                 758     388      75
 
Bpu10I CC'TnA_GC

Cuts at:      0     385    1221 
   Size:        385     836
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1086    1221 
   Size:       1086     135
 
BsaAI yAC'GTr

Cuts at:      0     674    1221 
   Size:        674     547
 
BsaHI Gr'CG_yC

Cuts at:      0      14     440    1221 
   Size:         14     426     781

  Fragments arranged by size: 

                 781     426      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      92     122    1221 
   Size:         92      30    1099

  Fragments arranged by size: 

                1099      92      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     773     920    1221 
   Size:        773     147     301

  Fragments arranged by size: 

                 773     301     147
 
BseYI C'CCAG_C

Cuts at:      0     650    1096    1221 
   Size:        650     446     125

  Fragments arranged by size: 

                 650     446     125
 
BsiEI CG_ry'CG

Cuts at:      0     289    1221 
   Size:        289     932
 
BsiHKAI G_wGCw'C

Cuts at:      0      61     386    1221 
   Size:         61     325     835

  Fragments arranged by size: 

                 835     325      61
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     986    1221 
   Size:        986     235
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     386     635     765     858    1150    1221 
   Size:         61     325     249     130      93     292      71

  Fragments arranged by size: 

                 325     292     249     130      93      71      61
 
BspHI T'CATG_A

Cuts at:      0     122     428    1221 
   Size:        122     306     793

  Fragments arranged by size: 

                 793     306     122
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1039    1221 
   Size:       1039     182
 
BsrFI r'CCGG_y

Cuts at:      0     711    1156    1221 
   Size:        711     445      65

  Fragments arranged by size: 

                 711     445      65
 
Bsu36I CC'TnA_GG

Cuts at:      0     842    1221 
   Size:        842     379
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     170     205     685     720    1221 
   Size:        170      35     480      35     501

  Fragments arranged by size: 

                 501     480     170      35      35
 
DraI TTT'AAA

Cuts at:      0     174    1221 
   Size:        174    1047
 
DraIII CAC_nnn'GTG

Cuts at:      0    1194    1221 
   Size:       1194      27
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     508    1221 
   Size:        508     713
 
EaeI y'GGCC_r

Cuts at:      0     203     228     286    1221 
   Size:        203      25      58     935

  Fragments arranged by size: 

                 935     203      58      25
 
EagI C'GGCC_G

Cuts at:      0     286    1221 
   Size:        286     935
 
EarI CTCTTCn'nnn_

Cuts at:      0      40     750    1221 
   Size:         40     710     471

  Fragments arranged by size: 

                 710     471      40
 
EcoICRI GAG'CTC

Cuts at:      0     384    1221 
   Size:        384     837
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     833    1221 
   Size:         75     758     388

  Fragments arranged by size: 

                 758     388      75
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     812    1221 
   Size:        812     409
 
EcoO109I rG'GnC_Cy

Cuts at:      0     310     455    1123    1146    1147    1221 
   Size:        310     145     668      23       1      74

  Fragments arranged by size: 

                 668     310     145      74      23       1
 
EcoRI G'AATT_C

Cuts at:      0     356    1221 
   Size:        356     865
 
FspI TGC'GCA

Cuts at:      0      79    1221 
   Size:         79    1142
 
HaeII r_GCGC'y

Cuts at:      0      17     592    1221 
   Size:         17     575     629

  Fragments arranged by size: 

                 629     575      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     478     510    1221 
   Size:        478      32     711

  Fragments arranged by size: 

                 711     478      32
 
HincII GTy'rAC

Cuts at:      0     513    1221 
   Size:        513     708
 
KasI G'GCGC_C

Cuts at:      0      13    1221 
   Size:         13    1208
 
MfeI C'AATT_G

Cuts at:      0     366    1221 
   Size:        366     855
 
MscI TGG'CCA

Cuts at:      0     205    1221 
   Size:        205    1016
 
MslI CAynn'nnrTG

Cuts at:      0     368    1221 
   Size:        368     853
 
MspA1I CmG'CkG

Cuts at:      0    1166    1221 
   Size:       1166      55
 
NaeI GCC'GGC

Cuts at:      0     713    1158    1221 
   Size:        713     445      63

  Fragments arranged by size: 

                 713     445      63
 
NarI GG'CG_CC

Cuts at:      0      14    1221 
   Size:         14    1207
 
NgoMIV G'CCGG_C

Cuts at:      0     711    1156    1221 
   Size:        711     445      65

  Fragments arranged by size: 

                 711     445      65
 
NsiI A_TGCA'T

Cuts at:      0     521    1221 
   Size:        521     700
 
NspI r_CATG'y

Cuts at:      0     519    1221 
   Size:        519     702
 
PasI CC'CwG_GG

Cuts at:      0     831    1221 
   Size:        831     390
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     614     650    1221 
   Size:        614      36     571

  Fragments arranged by size: 

                 614     571      36
 
PfoI T'CCnGG_A

Cuts at:      0     810    1221 
   Size:        810     411
 
PmlI CAC'GTG

Cuts at:      0     674    1221 
   Size:        674     547
 
PpuMI rG'GwC_Cy

Cuts at:      0     310    1123    1221 
   Size:        310     813      98

  Fragments arranged by size: 

                 813     310      98
 
PspOMI G'GGCC_C

Cuts at:      0    1146    1221 
   Size:       1146      75
 
PstI C_TGCA'G

Cuts at:      0     753    1221 
   Size:        753     468
 
SacI G_AGCT'C

Cuts at:      0     386    1221 
   Size:        386     835
 
SanDI GG'GwC_CC

Cuts at:      0    1123    1221 
   Size:       1123      98
 
SapI GCTCTTCn'nnn_

Cuts at:      0      40    1221 
   Size:         40    1181
 
SbfI CC_TGCA'GG

Cuts at:      0     753    1221 
   Size:        753     468
 
SfcI C'TryA_G

Cuts at:      0     749    1221 
   Size:        749     472
 
SfoI GGC'GCC

Cuts at:      0      15    1221 
   Size:         15    1206
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1156    1221 
   Size:       1156      65
 
SmaI CCC'GGG

Cuts at:      0     262     948    1221 
   Size:        262     686     273

  Fragments arranged by size: 

                 686     273     262
 
SmlI C'TyrA_G

Cuts at:      0     149    1065    1221 
   Size:        149     916     156

  Fragments arranged by size: 

                 916     156     149
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     696    1221 
   Size:        696     525
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     692     724    1221 
   Size:        692      32     497

  Fragments arranged by size: 

                 692     497      32
 
XhoI C'TCGA_G

Cuts at:      0     149    1221 
   Size:        149    1072
 
XmaI C'CCGG_G

Cuts at:      0     260     946    1221 
   Size:        260     686     275

  Fragments arranged by size: 

                 686     275     260
 
XmnI GAAnn'nnTTC

Cuts at:      0      34    1221 
   Size:         34    1187
 

 Enzymes that do cut:

     AleI     ApaI     ApoI     AvaI     BanI    BanII     BbeI    BfrBI
     BglI     BlpI Bme1580I     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI
    BspMI    BsrFI   Bsu36I    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI
    HaeII    Hin4I   HincII     KasI     MfeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NsiI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI   PspOMI     PstI     SacI    SanDI     SapI     SbfI
     SfcI     SfoI    SgrAI     SmaI     SmlI    TaqII     TstI     XhoI
     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI     BcgI
    BciVI     BclI    BglII    BmgBI     BmrI     BmtI     BplI     BsaI
    BsaBI    BsaWI     BsgI    BsiWI     BsmI    BspEI    BsrBI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     BtsI     ClaI     EciI    EcoRV     FalI
     FseI    FspAI  HindIII     HpaI     KpnI     MluI     MmeI     NcoI
     NdeI     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII
     SalI     ScaI    SexAI     SfiI     SgfI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     ZraI
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