Restriction map for pF1KB6823
##################################
# NO CUT ENZYMES (  180 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AfaI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AviII       AvrII       
BanI        BbeI        BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DrdI        DsaI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       KasI        
KpnI        Ksp632I     KspI        MaeI        MboII       
MfeI        MluI        MroI        MseI        MslI        
MspI        MunI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AlwNI         369  CAGAAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI          68  GTGCTC               G[AT]GC[AT]^C
AvaII         234  GGACC                G^G[AT]CC
BalI            2  TGGCCA               TGG^CCA
BamHI          47  GGATCC               G^GATCC
BanII         482  GAGCCC               G[GA]GC[CT]^C
BbrPI         420  CACGTG               CAC^GTG
BsaAI         420  CACGTG               [CT]AC^GT[GA]
BsaBI         269  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI          11  CCAAGG               C^C[ACGT][ACGT]GG
BsgI          389  GTGCAG               GTGCAG(16/14)
BsiHKAI        68  GTGCTC               G[AT]GC[AT]^C
BspMI         131  ACCTGC               ACCTGC(4/8)
BsrDI         313  CATTGC               GCAATG(2/0)
BstAPI         79  GCAGCCCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I         467  GCCAGC               GC[ACGT]^[ACGT]GC
CfoI           42  GCGC                 GCG^C
DraIII        472  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI            2  TGGCCA               [CT]^GGCC[GA]
EcoT14I        11  CCAAGG               C^C[AT][AT]GG
HhaI           42  GCGC                 GCG^C
HinP1I         42  GCGC                 G^CGC
MamI          269  GATTTACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MspA1I        306  CAGCTG               C[AC]G^C[GT]G
PleI          325  GAGTC                GAGTC(4/5)
PmaCI         420  CACGTG               CAC^GTG
PmlI          420  CACGTG               CAC^GTG
PvuII         306  CAGCTG               CAG^CTG
StyI           11  CCAAGG               C^C[AT][AT]GG
TaqI          323  TCGA                 T^CGA
TfiI          320  GATTC                G^A[AT]TC
TthHB8I       323  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

BmyI           68  GTGCTC               G[AGT]GC[ACT]^C
              482  GAGCCC              
BsiYI         172  CCAAAGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              237  CCATGCAAAGG         
BslI          172  CCAAAGAGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              237  CCATGCAAAGG         
BsmAI         260  GAGAC                GTCTC(1/5)
              429  GTCTC               
BsmFI         180  GGGAC                GGGAC(10/14)
              233  GGGAC               
Bsp1286I       68  GTGCTC               G[AGT]GC[ACT]^C
              482  GAGCCC              
BsrI          293  ACTGG                ACTGG(1/-1)
               65  CCAGT               
BssKI          51  CCAGG                ^CC[ACGT]GG
              457  CCAGG               
BstNI          51  CCAGG                CC^[AT]GG
              457  CCAGG               
BstYI          47  GGATCC               [GA]^GATC[CT]
              444  GGATCT              
Cfr13I        234  GGACC                G^G[ACGT]CC
              296  GGGCC               
DdeI          108  CTCAG                C^T[ACGT]AG
              154  CTGAG               
DpnII          48  GATC                 ^GATC
              445  GATC                
EcoRII         51  CCAGG                ^CC[AT]GG
              457  CCAGG               
HaeIII          3  GGCC                 GG^CC
              297  GGCC                
HinfI         320  GATTC                G^A[ACGT]TC
              325  GAGTC               
HphI          382  GGTGA                GGTGA(8/7)
              114  TCACC               
MboI           48  GATC                 ^GATC
              445  GATC                
MflI           47  GGATCC               [GA]^GATC[CT]
              444  GGATCT              
MvaI           51  CCAGG                CC^[AT]GG
              457  CCAGG               
NdeII          48  GATC                 ^GATC
              445  GATC                
PstI           77  CTGCAG               CTGCA^G
              253  CTGCAG              
Sau3AI         48  GATC                 ^GATC
              445  GATC                
Sau96I        234  GGACC                G^G[ACGT]CC
              296  GGGCC               
ScrFI          51  CCAGG                CC^[ACGT]GG
              457  CCAGG               
SfaNI          31  GCATC                GCATC(5/9)
              453  GCATC               
SfcI           77  CTGCAG               C^T[GA][CT]AG
              253  CTGCAG              
Tsp45I         16  GTCAC                ^GT[GC]AC
              327  GTCAC               
XhoII          47  GGATCC               [GA]^GATC[CT]
              444  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   15 enzymes) #
#########################################

AluI        AlwI        BbvI        BstF5I      Fnu4HI      
FokI        ItaI        MaeII       MaeIII      MnlI        
MwoI        NlaIII      NlaIV       TseI        TspRI       
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