Restriction map for pF1KB6841
 (Linear) (Six-base) MAPSORT of: KIBB6841.seq  Check: 3171  from: 1  to: 516



KIBB6841.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     302     377     516 
   Size:        302      75     139

  Fragments arranged by size: 

                 302     139      75
 
AflIII A'CryG_T

Cuts at:      0       7     516 
   Size:          7     509
 
AhdI GACnn_n'nnGTC

Cuts at:      0     459     516 
   Size:        459      57
 
AlwNI CAG_nnn'CTG

Cuts at:      0     158     378     516 
   Size:        158     220     138

  Fragments arranged by size: 

                 220     158     138
 
AvaI C'yCGr_G

Cuts at:      0     299     403     499     516 
   Size:        299     104      96      17

  Fragments arranged by size: 

                 299     104      96      17
 
BanI G'GyrC_C

Cuts at:      0     418     477     516 
   Size:        418      59      39

  Fragments arranged by size: 

                 418      59      39
 
BanII G_rGCy'C

Cuts at:      0     212     404     500     516 
   Size:        212     192      96      16

  Fragments arranged by size: 

                 212     192      96      16
 
BbeI G_GCGC'C

Cuts at:      0     422     481     516 
   Size:        422      59      35

  Fragments arranged by size: 

                 422      59      35
 
BbvCI CC'TCA_GC

Cuts at:      0     322     516 
   Size:        322     194
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     256     290     516 
   Size:        256      34     226

  Fragments arranged by size: 

                 256     226      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     475     516 
   Size:        475      41
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     516 
   Size:        403     113
 
Bpu10I CC'TnA_GC

Cuts at:      0     322     516 
   Size:        322     194
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     281     516 
   Size:        281     235
 
BsaHI Gr'CG_yC

Cuts at:      0     128     419     478     516 
   Size:        128     291      59      38

  Fragments arranged by size: 

                 291     128      59      38
 
BseYI C'CCAG_C

Cuts at:      0     137     428     487     516 
   Size:        137     291      59      29

  Fragments arranged by size: 

                 291     137      59      29
 
BsiEI CG_ry'CG

Cuts at:      0     416     516 
   Size:        416     100
 
BsmI GAATG_Cn'

Cuts at:      0     448     516 
   Size:        448      68
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     328     516 
   Size:        328     188
 
Bsp1286I G_dGCh'C

Cuts at:      0     212     404     500     516 
   Size:        212     192      96      16

  Fragments arranged by size: 

                 212     192      96      16
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     302     377     516 
   Size:        302      75     139

  Fragments arranged by size: 

                 302     139      75
 
BsrBI CCG'CTC

Cuts at:      0     243     516 
   Size:        243     273
 
BsrFI r'CCGG_y

Cuts at:      0      15     415     516 
   Size:         15     400     101

  Fragments arranged by size: 

                 400     101      15
 
BssHII G'CGCG_C

Cuts at:      0     148     277     516 
   Size:        148     129     239

  Fragments arranged by size: 

                 239     148     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     158     180     252     378     516 
   Size:        158      22      72     126     138

  Fragments arranged by size: 

                 158     138     126      72      22
 
Bsu36I CC'TnA_GG

Cuts at:      0     106     516 
   Size:        106     410
 
BtgI C'CryG_G

Cuts at:      0      91     516 
   Size:         91     425
 
EaeI y'GGCC_r

Cuts at:      0     413     516 
   Size:        413     103
 
EagI C'GGCC_G

Cuts at:      0     413     516 
   Size:        413     103
 
EarI CTCTTCn'nnn_

Cuts at:      0     477     516 
   Size:        477      39
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      34     454     516 
   Size:         34     420      62

  Fragments arranged by size: 

                 420      62      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     403     516 
   Size:        403     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0      74      99     516 
   Size:         74      25     417

  Fragments arranged by size: 

                 417      74      25
 
FspI TGC'GCA

Cuts at:      0      49     162     516 
   Size:         49     113     354

  Fragments arranged by size: 

                 354     113      49
 
HaeII r_GCGC'y

Cuts at:      0     203     321     390     422     481     516 
   Size:        203     118      69      32      59      35

  Fragments arranged by size: 

                 203     118      69      59      35      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     445     477     516 
   Size:        445      32      39

  Fragments arranged by size: 

                 445      39      32
 
KasI G'GCGC_C

Cuts at:      0     418     477     516 
   Size:        418      59      39

  Fragments arranged by size: 

                 418      59      39
 
MluI A'CGCG_T

Cuts at:      0       7     516 
   Size:          7     509
 
MslI CAynn'nnrTG

Cuts at:      0     168     516 
   Size:        168     348
 
MspA1I CmG'CkG

Cuts at:      0      93     155     192     249     516 
   Size:         93      62      37      57     267

  Fragments arranged by size: 

                 267      93      62      57      37
 
NaeI GCC'GGC

Cuts at:      0     417     516 
   Size:        417      99
 
NarI GG'CG_CC

Cuts at:      0     419     478     516 
   Size:        419      59      38

  Fragments arranged by size: 

                 419      59      38
 
NgoMIV G'CCGG_C

Cuts at:      0     415     516 
   Size:        415     101
 
PasI CC'CwG_GG

Cuts at:      0      60     516 
   Size:         60     456
 
PpuMI rG'GwC_Cy

Cuts at:      0      74      99     516 
   Size:         74      25     417

  Fragments arranged by size: 

                 417      74      25
 
PspXI vC'TCGA_Gb

Cuts at:      0     403     516 
   Size:        403     113
 
PstI C_TGCA'G

Cuts at:      0     374     516 
   Size:        374     142
 
SacII CC_GC'GG

Cuts at:      0      94     516 
   Size:         94     422
 
SanDI GG'GwC_CC

Cuts at:      0      74      99     516 
   Size:         74      25     417

  Fragments arranged by size: 

                 417      74      25
 
SbfI CC_TGCA'GG

Cuts at:      0     374     516 
   Size:        374     142
 
ScaI AGT'ACT

Cuts at:      0     178     516 
   Size:        178     338
 
SfcI C'TryA_G

Cuts at:      0     370     516 
   Size:        370     146
 
SfoI GGC'GCC

Cuts at:      0     420     479     516 
   Size:        420      59      37

  Fragments arranged by size: 

                 420      59      37
 
SmaI CCC'GGG

Cuts at:      0     301     516 
   Size:        301     215
 
SmlI C'TyrA_G

Cuts at:      0     403     516 
   Size:        403     113
 
StuI AGG'CCT

Cuts at:      0     394     469     516 
   Size:        394      75      47

  Fragments arranged by size: 

                 394      75      47
 
StyI C'CwwG_G

Cuts at:      0     464     516 
   Size:        464      52
 
TatI w'GTAC_w

Cuts at:      0     176     516 
   Size:        176     340
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     118     150     516 
   Size:        118      32     366

  Fragments arranged by size: 

                 366     118      32
 
XhoI C'TCGA_G

Cuts at:      0     403     516 
   Size:        403     113
 
XmaI C'CCGG_G

Cuts at:      0     299     516 
   Size:        299     217
 

 Enzymes that do cut:

     AarI   AflIII     AhdI    AlwNI     AvaI     BanI    BanII     BbeI
    BbvCI     BcgI     BglI     BpmI   Bpu10I     BsaI    BsaHI    BseYI
    BsiEI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII
   BstAPI   Bsu36I     BtgI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I     FspI    HaeII    Hin4I     KasI     MluI     MslI   MspA1I
     NaeI     NarI   NgoMIV     PasI    PpuMI    PspXI     PstI    SacII
    SanDI     SbfI     ScaI     SfcI     SfoI     SmaI     SmlI     StuI
     StyI     TatI     TstI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbsI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI    BsiWI
    BspEI    BspHI    BsrDI    BsrGI    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MmeI     MscI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PvuI    PvuII    RsrII     SacI     SalI     SapI    SexAI
     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII  Tth111I     XbaI     XcmI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com