Restriction map for pF1KB6745
##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfaI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmAI       BsmBI       BsmI        BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      ClaI        CpoI        DdeI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FokI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HinfI       
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MamI        MfeI        
MflI        MluI        MluNI       MroI        MscI        
MslI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NlaIII      
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        PvuII       RcaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tsp45I      Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AcsI          242  AAATTT               [GA]^AATT[CT]
AfaI          270  GTAC                 GT^AC
AlwNI         118  CAGTGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI        88  AGCGCT               AGC^GCT
ApoI          242  AAATTT               [GA]^AATT[CT]
AspHI           3  GAGCTC               G[AT]GC[AT]^C
AvaI           34  CCCGGG               C^[CT]CG[GA]G
BanII           3  GAGCTC               G[GA]GC[CT]^C
BbsI          132  GAAGAC               GAAGAC(2/6)
BclI          179  TGATCA               T^GATCA
BmyI            3  GAGCTC               G[AGT]GC[ACT]^C
BpmI          172  CTGGAG               CTGGAG(16/14)
BpuAI         132  GAAGAC               GAAGAC(2/6)
BsiHKAI         3  GAGCTC               G[AT]GC[AT]^C
BsiYI           8  CCGCAGCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI            8  CCGCAGCCGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI          34  CCCGGG               C^[CT]CG[GA]G
Bsp1286I        3  GAGCTC               G[AGT]GC[ACT]^C
Bsp1407I      269  TGTACA               T^GTACA
BsrGI         269  TGTACA               T^GTACA
DpnII         180  GATC                 ^GATC
DraII         110  GGGACCT              [GA]G^G[ACGT]CC[CT]
Eco47III       88  AGCGCT               AGC^GCT
EcoO109I      110  GGGACCT              [GA]G^G[ACGT]CC[CT]
FauI          264  CCCGC                CCCGC(4/6)
FbaI          179  TGATCA               T^GATCA
HaeII          88  AGCGCT               [GA]GCGC^[CT]
HaeIII        186  GGCC                 GG^CC
MboI          180  GATC                 ^GATC
MseI          284  TTAA                 T^TAA
MspA1I        265  CCGCTG               C[AC]G^C[GT]G
NdeII         180  GATC                 ^GATC
PpuMI         110  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          115  CTGCAG               CTGCA^G
RsaI          270  GTAC                 GT^AC
SacI            3  GAGCTC               GAGCT^C
Sau3AI        180  GATC                 ^GATC
SfcI          115  CTGCAG               C^T[GA][CT]AG
SmaI           34  CCCGGG               CCC^GGG
SspBI         269  TGTACA               T^GTACA
Tru9I         284  TTAA                 T^TAA
XmaCI          34  CCCGGG               C^CCGGG
XmaI           34  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

AciI            8  CCGC                 CCGC(-3/-1)
              265  CCGC                
AluI            4  AGCT                 AG^CT
               96  AGCT                
BsmFI         110  GGGAC                GGGAC(10/14)
              152  GTCCC               
BspMI         113  ACCTGC               ACCTGC(4/8)
              254  ACCTGC              
Cac8I          85  GCCAGC               GC[ACGT]^[ACGT]GC
              258  GCAAGC              
CfoI           23  GCGC                 GCG^C
               89  GCGC                
HhaI           23  GCGC                 GCG^C
               89  GCGC                
HinP1I         23  GCGC                 G^CGC
               89  GCGC                
HphI          177  GGTGA                GGTGA(8/7)
              164  TCACC               
NlaIV         110  GGGACC               GG[ACGT]^[ACGT]CC
              190  GGACCC              
Tsp509I       243  AATT                 ^AATT
              282  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AvaII       BbvI        BcnI        BsaJI       BssKI       
BstNI       Cfr13I      EcoRII      Fnu4HI      HapII       
HpaII       ItaI        MboII       MnlI        MspI        
MvaI        MwoI        NciI        Sau96I      ScrFI       
TseI        TspRI       
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