Restriction map for pF1KB6423
##################################
# NO CUT ENZYMES (  156 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BbeI        BbrPI       BbvCI       BcgI        BclI        
BcnI        BfrI        BglII       BlnI        BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiWI       BsmAI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstEII      BstF5I      
BstPI       BstUI       BstYI       BstZ17I     CfoI        
ClaI        CpoI        DraI        DrdI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoRI       EcoRV       
FbaI        FokI        FseI        FspI        HaeII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HpaI        KasI        Ksp632I     
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MunI        MvnI        NaeI        NarI        NciI        
NgoMIV      NotI        NruI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PvuI        RcaI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XhoII       XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AlwI          397  GGATC                GGATC(4/5)
AocI          853  CCTGAGG              CC^T[ACGT]AGG
AspHI         316  GAGCTC               G[AT]GC[AT]^C
AvaII         481  GGTCC                G^G[AT]CC
BbsI          346  GTCTTC               GAAGAC(2/6)
BciVI         303  GTATCC               GTATCC(6/5)
BlpI          764  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I      764  GCTTAGC              GC^T[ACGT]AGC
BpuAI         346  GTCTTC               GAAGAC(2/6)
BsiHKAI       316  GAGCTC               G[AT]GC[AT]^C
BsiYI         472  CCCTCAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          472  CCCTCAAAGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          632  GAATGC               GAATGC(1/-1)
BsrDI         368  CATTGC               GCAATG(2/0)
BsrFI         122  ACCGGC               [GA]^CCGG[CT]
BsrI          736  ACTGG                ACTGG(1/-1)
BstBI         439  TTCGAA               TT^CGAA
BstXI         447  CCACAGCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I        853  CCTGAGG              CC^T[ACGT]AGG
CelII         764  GCTTAGC              GC^T[ACGT]AGC
Cfr10I        122  ACCGGC               [GA]^CCGG[CT]
DraIII        523  CACTCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I        853  CCTGAGG              CC^T[ACGT]AGG
EcoT14I       453  CCATGG               C^C[AT][AT]GG
EcoT22I       184  ATGCAT               ATGCA^T
FauI          759  GCGGG                CCCGC(4/6)
HapII         123  CCGG                 C^CGG
HpaII         123  CCGG                 C^CGG
MspI          123  CCGG                 C^CGG
NcoI          453  CCATGG               C^CATGG
NsiI          184  ATGCAT               ATGCA^T
NspV          439  TTCGAA               TT^CGAA
PpuMI         480  GGGTCCC              [GA]G^G[AT]CC[CT]
PstI          553  CTGCAG               CTGCA^G
PvuII         794  CAGCTG               CAG^CTG
SacI          316  GAGCTC               GAGCT^C
SfcI          553  CTGCAG               C^T[GA][CT]AG
SfuI          439  TTCGAA               TT^CGAA
StyI          453  CCATGG               C^C[AT][AT]GG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Acc65I        652  GGTACC               G^GTACC
              799  GGTACC              
AcsI          267  AAATTC               [GA]^AATT[CT]
              827  AAATTC              
AfaI          653  GTAC                 GT^AC
              800  GTAC                
ApoI          267  AAATTC               [GA]^AATT[CT]
              827  AAATTC              
Asp700I       266  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              826  GAAATTCTTC          
Asp718I       652  GGTACC               G^GTACC
              799  GGTACC              
BanI          652  GGTACC               G^G[CT][GA]CC
              799  GGTACC              
DpnII         251  GATC                 ^GATC
              398  GATC                
DraII         480  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              857  AGGCCCC             
DsaI          453  CCATGG               C^C[GA][CT]GG
              835  CCACGG              
EcoO109I      480  GGGTCCC              [GA]G^G[ACGT]CC[CT]
              857  AGGCCCC             
KpnI          652  GGTACC               GGTAC^C
              799  GGTACC              
MboI          251  GATC                 ^GATC
              398  GATC                
MspA1I        794  CAGCTG               C[AC]G^C[GT]G
              887  CCGCTG              
NdeI          187  CATATG               CA^TATG
              295  CATATG              
NdeII         251  GATC                 ^GATC
              398  GATC                
NheI          540  GCTAGC               G^CTAGC
              843  GCTAGC              
RsaI          653  GTAC                 GT^AC
              800  GTAC                
Sau3AI        251  GATC                 ^GATC
              398  GATC                
SfaNI         103  GATGC                GCATC(5/9)
              280  GATGC               
TaqI          400  TCGA                 T^CGA
              440  TCGA                
TthHB8I       400  TCGA                 T^CGA
              440  TCGA                
XmnI          266  GAAATTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              826  GAAATTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BfaI        
BglI        BmyI        BpmI        BsaJI       BsgI        
BsmFI       Bsp1286I    BssKI       BstNI       Cac8I       
Cfr13I      DdeI        EcoRII      Fnu4HI      HaeIII      
HinfI       HphI        ItaI        MaeI        MaeIII      
MboII       MnlI        MslI        MvaI        MwoI        
NlaIII      NlaIV       PleI        Sau96I      ScrFI       
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
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