Restriction map for pF1KB6423
 (Linear) (Six-base) MAPSORT of: KIBB6423.seq  Check: 3703  from: 1  to: 927



KIBB6423.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:20  ..

Acc65I G'GTAC_C

Cuts at:      0     652     799     927 
   Size:        652     147     128

  Fragments arranged by size: 

                 652     147     128
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     770     927 
   Size:        218     552     157

  Fragments arranged by size: 

                 552     218     157
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     209     390     927 
   Size:        209     181     537

  Fragments arranged by size: 

                 537     209     181
 
ApoI r'AATT_y

Cuts at:      0     267     827     927 
   Size:        267     560     100

  Fragments arranged by size: 

                 560     267     100
 
BanI G'GyrC_C

Cuts at:      0     652     799     927 
   Size:        652     147     128

  Fragments arranged by size: 

                 652     147     128
 
BanII G_rGCy'C

Cuts at:      0     320     432     495     927 
   Size:        320     112      63     432

  Fragments arranged by size: 

                 432     320     112      63
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     339     927 
   Size:        339     588
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     314     927 
   Size:        314     613
 
BfrBI ATG'CAT

Cuts at:      0     186     927 
   Size:        186     741
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     904     922     927 
   Size:         46     858      18       5

  Fragments arranged by size: 

                 858      46      18       5
 
BlpI GC'TnA_GC

Cuts at:      0     765     927 
   Size:        765     162
 
BmtI G_CTAG'C

Cuts at:      0     544     847     927 
   Size:        544     303      80

  Fragments arranged by size: 

                 544     303      80
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     226     413     758     927 
   Size:        226     187     345     169

  Fragments arranged by size: 

                 345     226     187     169
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     427     598     927 
   Size:        262     165     171     329

  Fragments arranged by size: 

                 329     262     171     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     320     927 
   Size:        320     607
 
BsmI GAATG_Cn'

Cuts at:      0     638     927 
   Size:        638     289
 
Bsp1286I G_dGCh'C

Cuts at:      0     320     432     495     927 
   Size:        320     112      63     432

  Fragments arranged by size: 

                 432     320     112      63
 
BsrDI GCAATG_nn'

Cuts at:      0     367     927 
   Size:        367     560
 
BsrFI r'CCGG_y

Cuts at:      0     122     927 
   Size:        122     805
 
BstBI TT'CG_AA

Cuts at:      0     440     927 
   Size:        440     487
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     454     927 
   Size:        454     473
 
Bsu36I CC'TnA_GG

Cuts at:      0     854     927 
   Size:        854      73
 
BtgI C'CryG_G

Cuts at:      0     453     835     927 
   Size:        453     382      92

  Fragments arranged by size: 

                 453     382      92
 
BtsI GCAGTG_nn'

Cuts at:      0     379     677     927 
   Size:        379     298     250

  Fragments arranged by size: 

                 379     298     250
 
DraIII CAC_nnn'GTG

Cuts at:      0     528     927 
   Size:        528     399
 
EcoICRI GAG'CTC

Cuts at:      0     318     927 
   Size:        318     609
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     226     413     758     770     927 
   Size:        218       8     187     345      12     157

  Fragments arranged by size: 

                 345     218     187     157      12       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     481     858     927 
   Size:        481     377      69

  Fragments arranged by size: 

                 481     377      69
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     305     337     482     514     927 
   Size:        305      32     145      32     413

  Fragments arranged by size: 

                 413     305     145      32      32
 
KpnI G_GTAC'C

Cuts at:      0     656     803     927 
   Size:        656     147     124

  Fragments arranged by size: 

                 656     147     124
 
MslI CAynn'nnrTG

Cuts at:      0     187     372     452     927 
   Size:        187     185      80     475

  Fragments arranged by size: 

                 475     187     185      80
 
MspA1I CmG'CkG

Cuts at:      0     796     889     927 
   Size:        796      93      38

  Fragments arranged by size: 

                 796      93      38
 
NcoI C'CATG_G

Cuts at:      0     453     927 
   Size:        453     474
 
NdeI CA'TA_TG

Cuts at:      0     188     296     927 
   Size:        188     108     631

  Fragments arranged by size: 

                 631     188     108
 
NheI G'CTAG_C

Cuts at:      0     540     843     927 
   Size:        540     303      84

  Fragments arranged by size: 

                 540     303      84
 
NsiI A_TGCA'T

Cuts at:      0     188     927 
   Size:        188     739
 
PasI CC'CwG_GG

Cuts at:      0     330     708     927 
   Size:        330     378     219

  Fragments arranged by size: 

                 378     330     219
 
PpuMI rG'GwC_Cy

Cuts at:      0     481     927 
   Size:        481     446
 
PstI C_TGCA'G

Cuts at:      0     557     927 
   Size:        557     370
 
PvuII CAG'CTG

Cuts at:      0     796     927 
   Size:        796     131
 
SacI G_AGCT'C

Cuts at:      0     320     927 
   Size:        320     607
 
SanDI GG'GwC_CC

Cuts at:      0     481     927 
   Size:        481     446
 
SfcI C'TryA_G

Cuts at:      0     553     927 
   Size:        553     374
 
StyI C'CwwG_G

Cuts at:      0     453     927 
   Size:        453     474
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     389     421     927 
   Size:        389      32     506

  Fragments arranged by size: 

                 506     389      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     270     830     927 
   Size:        270     560      97

  Fragments arranged by size: 

                 560     270      97
 

 Enzymes that do cut:

   Acc65I     AcuI     AlfI     ApoI     BanI    BanII     BbsI    BciVI
    BfrBI     BglI     BlpI     BmtI     BpmI     BsgI  BsiHKAI     BsmI
 Bsp1286I    BsrDI    BsrFI    BstBI    BstXI   Bsu36I     BtgI     BtsI
   DraIII  EcoICRI  Eco57MI EcoO109I    Hin4I     KpnI     MslI   MspA1I
     NcoI     NdeI     NheI     NsiI     PasI    PpuMI     PstI    PvuII
     SacI    SanDI     SfcI     StyI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
     BclI    BglII Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrGI
   BssHII    BssSI   BstAPI   BstEII    BstYI  BstZ17I    BtgZI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI     NaeI
     NarI   NgoMIV     NotI     NruI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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