Restriction map for pF1KE0621
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbsI        BbvCI       BcgI        BcnI        BfrI        
BglI        BglII       BlnI        BlpI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FseI        FspI        HapII       HgaI        Hin1I       
HindIII     HpaII       HphI        KasI        KpnI        
KspI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tth111I     Van91I      XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AciI          460  GCGG                 CCGC(-3/-1)
AlwNI         488  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvaII          96  GGACC                G^G[AT]CC
BanII          54  GGGCTC               G[GA]GC[CT]^C
BbvI          487  GCAGC                GCAGC(8/12)
BciVI         480  GTATCC               GTATCC(6/5)
BclI          333  TGATCA               T^GATCA
BfaI          545  CTAG                 C^TAG
BsaJI          26  CCCTGG               C^C[ACGT][ACGT]GG
BsiYI          45  CCTTTCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI           45  CCTTTCAGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         195  GGGAC                GGGAC(10/14)
BsmI           33  GAATGC               GAATGC(1/-1)
BsrI          258  CCAGT                ACTGG(1/-1)
Cac8I         249  GCAAGC               GC[ACGT]^[ACGT]GC
CfoI          490  GCGC                 GCG^C
DpnII         334  GATC                 ^GATC
EcoNI          45  CCTTTCAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I        35  ATGCAT               ATGCA^T
FbaI          333  TGATCA               T^GATCA
Fnu4HI        487  GCAGC                GC^[ACGT]GC
HaeII         489  AGCGCC               [GA]GCGC^[CT]
HaeIII        279  GGCC                 GG^CC
HhaI          490  GCGC                 GCG^C
HinP1I        490  GCGC                 G^CGC
HincII        220  GTTAAC               GT[CT]^[GA]AC
HindII        220  GTTAAC               GT[CT]^[GA]AC
HinfI         354  GATTC                G^A[ACGT]TC
HpaI          220  GTTAAC               GTT^AAC
ItaI          487  GCAGC                GC^[ACGT]GC
MaeI          545  CTAG                 C^TAG
MaeIII        415  GTCAC                ^GT[ACGT]AC
MboI          334  GATC                 ^GATC
NdeII         334  GATC                 ^GATC
NlaIV          96  GGACCC               GG[ACGT]^[ACGT]CC
NsiI           35  ATGCAT               ATGCA^T
NspI          312  ACATGC               [GA]CATG^[CT]
PstI           61  CTGCAG               CTGCA^G
Sau3AI        334  GATC                 ^GATC
SfcI           61  CTGCAG               C^T[GA][CT]AG
TfiI          354  GATTC                G^A[AT]TC
TseI          487  GCAGC                G^C[AT]GC
Tsp45I        415  GTCAC                ^GT[GC]AC
Tsp509I       105  AATT                 ^AATT
XbaI          544  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AluI          128  AGCT                 AG^CT
              293  AGCT                
AvaI          203  CTCGAG               C^[CT]CG[GA]G
              370  CTCGAG              
BmyI           54  GGGCTC               G[AGT]GC[ACT]^C
              308  GGGCAC              
BpmI          133  CTCCAG               CTGGAG(16/14)
               28  CTGGAG              
BseRI         121  CTCCTC               GAGGAG(10/8)
              130  CTCCTC              
BsmAI         263  GTCTC                GTCTC(1/5)
              368  GTCTC               
BsoBI         203  CTCGAG               C^[CT]CG[GA]G
              370  CTCGAG              
Bsp1286I       54  GGGCTC               G[AGT]GC[ACT]^C
              308  GGGCAC              
BssKI          27  CCTGG                ^CC[ACGT]GG
               93  CCTGG               
BstNI          27  CCTGG                CC^[AT]GG
               93  CCTGG               
BstXI         135  CCAGAAGTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              287  CCAAGAAGCTGG        
Cfr13I         96  GGACC                G^G[ACGT]CC
              279  GGCCC               
EarI           20  CTCTTC               CTCTTC(1/4)
              169  CTCTTC              
EcoRII         27  CCTGG                ^CC[AT]GG
               93  CCTGG               
Ksp632I        20  CTCTTC               CTCTTC(1/4)
              169  CTCTTC              
MseI          221  TTAA                 T^TAA
              509  TTAA                
MvaI           27  CCTGG                CC^[AT]GG
               93  CCTGG               
NlaIII        313  CATG                 CATG^
              554  CATG                
PaeR7I        203  CTCGAG               C^TCGAG
              370  CTCGAG              
Sau96I         96  GGACC                G^G[ACGT]CC
              279  GGCCC               
ScrFI          27  CCTGG                CC^[ACGT]GG
               93  CCTGG               
SfaNI         472  GATGC                GCATC(5/9)
               37  GCATC               
Tru9I         221  TTAA                 T^TAA
              509  TTAA                
XcmI          258  CCAGTGTCTCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              287  CCAAGAAGCTGGTGG     
XhoI          203  CTCGAG               C^TCGAG
              370  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

BstF5I      DdeI        FokI        MboII       MnlI        
MwoI        SmlI        TaqI        TspRI       TthHB8I     
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