Restriction map for pF1KB0936
##################################
# NO CUT ENZYMES (  163 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BpuAI       
BsaAI       BsaBI       BsaHI       BsaI        BseAI       
BsgI        BsiEI       BsiWI       BsmBI       BsoBI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        ClaI        CpoI        
DraIII      EaeI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO65I     
EcoRI       EcoT22I     FauI        FbaI        FseI        
FspI        HaeII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MaeII       MamI        
MflI        MluI        MluNI       MroI        MscI        
MspA1I      MvnI        NaeI        NarI        NciI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StuI        SwaI        
TseI        Tth111I     XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   34 enzymes) #
###################################

AspHI         406  GAGCTC               G[AT]GC[AT]^C
BpmI          958  CTGGAG               CTGGAG(16/14)
BsaWI          37  ACCGGA               [AT]^CCGG[AT]
BsiHKAI       406  GAGCTC               G[AT]GC[AT]^C
BsmAI        1042  GAGAC                GTCTC(1/5)
BsmI          878  GCATTC               GAATGC(1/-1)
Bsp1407I     1031  TGTACA               T^GTACA
BspMI         729  GCAGGT               ACCTGC(4/8)
BsrDI         239  CATTGC               GCAATG(2/0)
BsrFI         387  GCCGGT               [GA]^CCGG[CT]
BsrGI        1031  TGTACA               T^GTACA
BstXI         131  CCAATAATCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I        387  GCCGGT               [GA]^CCGG[CT]
DraI          111  TTTAAA               TTT^AAA
DrdI           85  GACCTAATAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          935  CCATGG               C^C[GA][CT]GG
EcoRV        1111  GATATC               GAT^ATC
EcoT14I       935  CCATGG               C^C[AT][AT]GG
Fnu4HI       1088  GCCGC                GC^[ACGT]GC
HaeIII        615  GGCC                 GG^CC
ItaI         1088  GCCGC                GC^[ACGT]GC
MfeI         1145  CAATTG               C^AATTG
MunI         1145  CAATTG               C^AATTG
NcoI          935  CCATGG               C^CATGG
NdeI          166  CATATG               CA^TATG
PflMI         838  CCACTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          521  GAGTC                GAGTC(4/5)
SacI          406  GAGCTC               GAGCT^C
SapI          403  GAAGAGC              GCTCTTC(1/4)
SspBI        1031  TGTACA               T^GTACA
StyI          935  CCATGG               C^C[AT][AT]GG
TfiI          783  GAATC                G^A[AT]TC
Van91I        838  CCACTAGATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          601  CCAGTGTCCCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcsI          320  AAATTC               [GA]^AATT[CT]
              907  GAATTT              
ApoI          320  AAATTC               [GA]^AATT[CT]
              907  GAATTT              
BanII         406  GAGCTC               G[GA]GC[CT]^C
              561  GAGCCC              
BfaI          473  CTAG                 C^TAG
              841  CTAG                
BmyI          406  GAGCTC               G[AGT]GC[ACT]^C
              561  GAGCCC              
BseRI         833  CTCCTC               GAGGAG(10/8)
              305  GAGGAG              
Bsp1286I      406  GAGCTC               G[AGT]GC[ACT]^C
              561  GAGCCC              
Cac8I        1080  GCTGGC               GC[ACGT]^[ACGT]GC
             1084  GCAAGC              
HapII          38  CCGG                 C^CGG
              388  CCGG                
HinfI         521  GAGTC                G^A[ACGT]TC
              783  GAATC               
HpaII          38  CCGG                 C^CGG
              388  CCGG                
HphI          904  GGTGA                GGTGA(8/7)
              932  TCACC               
MaeI          473  CTAG                 C^TAG
              841  CTAG                
MslI          765  CACTGAAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              893  CACATCAGTG          
MspI           38  CCGG                 C^CGG
              388  CCGG                
MwoI          370  GCTGTGAGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
             1091  GCATCTTGAGC         
NlaIII        348  CATG                 CATG^
              936  CATG                
SmlI          558  CTTGAG               C^T[CT][GA]AG
             1095  CTTGAG              
TaqI          303  TCGA                 T^CGA
              324  TCGA                
Tsp45I         94  GTCAC                ^GT[GC]AC
              865  GTGAC               
TthHB8I       303  TCGA                 T^CGA
              324  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BsaJI       BsiYI       BslI        BsmFI       
BsrI        BssKI       BstF5I      BstNI       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      FokI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MvaI        NdeII       
NlaIV       PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       Tru9I       Tsp509I     TspRI       
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