Restriction map for pF1KB7590
##################################
# NO CUT ENZYMES (  135 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        Alw44I      
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AspHI       AspI        
AviII       AvrII       BamHI       BbrPI       BbsI        
BciVI       BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaBI       BsaWI       
BseAI       BsgI        BsiHKAI     BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     CelII       ClaI        
CpoI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco52I      
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        Ksp632I     KspI        MamI        
MfeI        MluI        MroI        MseI        MunI        
NcoI        NdeI        NheI        NlaIII      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfcI        SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StyI        SwaI        TfiI        Tru9I       
Tth111I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AflIII        313  ACACGT               A^C[GA][CT]GT
AlwI          768  GGATC                GGATC(4/5)
AlwNI         846  CAGCAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI       365  AGCGCT               AGC^GCT
AvaI          202  CCCGGG               C^[CT]CG[GA]G
BalI          117  TGGCCA               TGG^CCA
BbeI           13  GGCGCC               GGCGC^C
BcgI          522  CGAGGCCTTTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI         314  CACGTA               [CT]AC^GT[GA]
BsiEI          24  CGACCG               CG[GA][CT]^CG
BsoBI         202  CCCGGG               C^[CT]CG[GA]G
BspMI         630  ACCTGC               ACCTGC(4/8)
BssHII         37  GCGCGC               G^CGCGC
BstEII        658  GGTGACC              G^GT[ACGT]ACC
BstF5I        320  CATCC                GGATG(2/0)
BstPI         658  GGTGACC              G^GT[ACGT]ACC
BstXI         811  CCATACAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         768  GGATCT               [GA]^GATC[CT]
DpnII         769  GATC                 ^GATC
Eco47III      365  AGCGCT               AGC^GCT
EcoNI         276  CCTGCCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       658  GGTGACC              G^GT[ACGT]ACC
FokI          320  CATCC                GGATG(9/13)
HinfI          49  GAGTC                G^A[ACGT]TC
KasI           13  GGCGCC               G^GCGCC
MaeII         315  ACGT                 A^CGT
MaeIII        659  GTGAC                ^GT[ACGT]AC
MboI          769  GATC                 ^GATC
MboII         471  GAAGA                GAAGA(8/7)
MflI          768  GGATCT               [GA]^GATC[CT]
MluNI         117  TGGCCA               TGG^CCA
MscI          117  TGGCCA               TGG^CCA
MslI          234  CATACTGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI           13  GGCGCC               GG^CGCC
NdeII         769  GATC                 ^GATC
PflMI         372  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           49  GAGTC                GAGTC(4/5)
PpuMI         142  GGGACCT              [GA]G^G[AT]CC[CT]
Sau3AI        769  GATC                 ^GATC
SfaNI         247  GATGC                GCATC(5/9)
SfoI           13  GGCGCC               GGC^GCC
SmaI          202  CCCGGG               CCC^GGG
TaqI          806  TCGA                 T^CGA
Tsp45I        659  GTGAC                ^GT[GC]AC
TthHB8I       806  TCGA                 T^CGA
Van91I        372  CCAGTATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         768  GGATCT               [GA]^GATC[CT]
XmaCI         202  CCCGGG               C^CCGGG
XmaI          202  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcyI           13  GGCGCC               G[GA]^CG[CT]C
              504  GGCGTC              
AfaI          317  GTAC                 GT^AC
              342  GTAC                
AocI          280  CCTAAGG              CC^T[ACGT]AGG
              653  CCTTAGG             
BanI           13  GGCGCC               G^G[CT][GA]CC
              646  GGCACC              
BsaHI          13  GGCGCC               G[GA]^CG[CT]C
              504  GGCGTC              
BseRI         510  CTCCTC               GAGGAG(10/8)
              672  CTCCTC              
BsrBI         760  CCGCTC               CCGCTC(-3/-3)
               89  GAGCGG              
Bsu36I        280  CCTAAGG              CC^T[ACGT]AGG
              653  CCTTAGG             
EaeI          117  TGGCCA               [CT]^GGCC[GA]
              137  TGGCCG              
Eco81I        280  CCTAAGG              CC^T[ACGT]AGG
              653  CCTTAGG             
HgaI          505  GCGTC                GACGC(5/10)
              709  GCGTC               
Hin1I          13  GGCGCC               G[GA]^CG[CT]C
              504  GGCGTC              
HphI          435  GGTGA                GGTGA(8/7)
              658  GGTGA               
NaeI          566  GCCGGC               GCC^GGC
              778  GCCGGC              
NgoMIV        566  GCCGGC               G^CCGGC
              778  GCCGGC              
RsaI          317  GTAC                 GT^AC
              342  GTAC                
StuI          524  AGGCCT               AGG^CCT
              624  AGGCCT              
Tsp509I       261  AATT                 ^AATT
              270  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AccII       AciI        AluI        ApaI        AvaII       
BanII       BbvCI       BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BsaI        BsaJI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrFI       BsrI        BssKI       BstNI       BstUI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DraII       EcoO109I    EcoRII      FauI        Fnu4HI      
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       ItaI        MaeI        MnlI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NlaIV       PvuII       Sau96I      ScrFI       TseI        
TspRI       
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