Restriction map for pF1KB7590
 (Linear) (Six-base) MAPSORT of: KIBB7590.seq  Check: 5204  from: 1  to: 870



KIBB7590.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:23  ..

AfeI AGC'GCT

Cuts at:      0     367     870 
   Size:        367     503
 
AflIII A'CryG_T

Cuts at:      0     313     870 
   Size:        313     557
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     240     256     870 
   Size:        240      16     614

  Fragments arranged by size: 

                 614     240      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     343     375     870 
   Size:        343      32     495

  Fragments arranged by size: 

                 495     343      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     851     870 
   Size:        851      19
 
ApaI G_GGCC'C

Cuts at:      0      34     209     591     870 
   Size:         34     175     382     279

  Fragments arranged by size: 

                 382     279     175      34
 
AvaI C'yCGr_G

Cuts at:      0     202     870 
   Size:        202     668
 
BanI G'GyrC_C

Cuts at:      0      13     646     870 
   Size:         13     633     224

  Fragments arranged by size: 

                 633     224      13
 
BanII G_rGCy'C

Cuts at:      0      34     209     591     870 
   Size:         34     175     382     279

  Fragments arranged by size: 

                 382     279     175      34
 
BbeI G_GCGC'C

Cuts at:      0      17     870 
   Size:         17     853
 
BbvCI CC'TCA_GC

Cuts at:      0     147     513     705     870 
   Size:        147     366     192     165

  Fragments arranged by size: 

                 366     192     165     147
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     511     545     870 
   Size:        511      34     325

  Fragments arranged by size: 

                 511     325      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     523     623     644     870 
   Size:        523     100      21     226

  Fragments arranged by size: 

                 523     226     100      21
 
Bme1580I G_kGCm'C

Cuts at:      0      34     209     584     591     649     870 
   Size:         34     175     375       7      58     221

  Fragments arranged by size: 

                 375     221     175      58      34       7
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     799     870 
   Size:        799      71
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     153     370     514     577     870 
   Size:        138      15     217     144      63     293

  Fragments arranged by size: 

                 293     217     144     138      63      15
 
Bpu10I CC'TnA_GC

Cuts at:      0     147     513     705     870 
   Size:        147     366     192     165

  Fragments arranged by size: 

                 366     192     165     147
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      71     421     482     870 
   Size:         71     350      61     388

  Fragments arranged by size: 

                 388     350      71      61
 
BsaAI yAC'GTr

Cuts at:      0     316     870 
   Size:        316     554
 
BsaHI Gr'CG_yC

Cuts at:      0      14     505     870 
   Size:         14     491     365

  Fragments arranged by size: 

                 491     365      14
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     343     373     870 
   Size:        343      30     497

  Fragments arranged by size: 

                 497     343      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     501     663     870 
   Size:        501     162     207

  Fragments arranged by size: 

                 501     207     162
 
BsiEI CG_ry'CG

Cuts at:      0      27     870 
   Size:         27     843
 
Bsp1286I G_dGCh'C

Cuts at:      0      34     209     584     591     649     870 
   Size:         34     175     375       7      58     221

  Fragments arranged by size: 

                 375     221     175      58      34       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     639     870 
   Size:        639     231
 
BsrBI CCG'CTC

Cuts at:      0      91     762     870 
   Size:         91     671     108

  Fragments arranged by size: 

                 671     108      91
 
BsrFI r'CCGG_y

Cuts at:      0     299     566     778     870 
   Size:        299     267     212      92

  Fragments arranged by size: 

                 299     267     212      92
 
BssHII G'CGCG_C

Cuts at:      0      37     870 
   Size:         37     833
 
BstEII G'GTnAC_C

Cuts at:      0     658     870 
   Size:        658     212
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     818     870 
   Size:        818      52
 
BstYI r'GATC_y

Cuts at:      0     768     870 
   Size:        768     102
 
Bsu36I CC'TnA_GG

Cuts at:      0     281     654     870 
   Size:        281     373     216

  Fragments arranged by size: 

                 373     281     216
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      20     870 
   Size:         20     850
 
BtsI GCAGTG_nn'

Cuts at:      0     222     326     718     870 
   Size:        222     104     392     152

  Fragments arranged by size: 

                 392     222     152     104
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     116     151     870 
   Size:        116      35     719

  Fragments arranged by size: 

                 719     116      35
 
EaeI y'GGCC_r

Cuts at:      0     117     137     870 
   Size:        117      20     733

  Fragments arranged by size: 

                 733     117      20
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      61     870 
   Size:         61     809
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     138     153     370     514     577     870 
   Size:        138      15     217     144      63     293

  Fragments arranged by size: 

                 293     217     144     138      63      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     280     870 
   Size:        280     590
 
EcoO109I rG'GnC_Cy

Cuts at:      0      30      31     143     285     587     588     870 
   Size:         30       1     112     142     302       1     282

  Fragments arranged by size: 

                 302     282     142     112      30       1       1
 
HaeII r_GCGC'y

Cuts at:      0      17      72     369     607     845     870 
   Size:         17      55     297     238     238      25

  Fragments arranged by size: 

                 297     238     238      55      25      17
 
KasI G'GCGC_C

Cuts at:      0      13     870 
   Size:         13     857
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     563     870 
   Size:        563     307
 
MscI TGG'CCA

Cuts at:      0     119     870 
   Size:        119     751
 
MslI CAynn'nnrTG

Cuts at:      0     238     870 
   Size:        238     632
 
MspA1I CmG'CkG

Cuts at:      0      86     339     414     693     739     818     851     870 
   Size:         86     253      75     279      46      79      33      19

  Fragments arranged by size: 

                 279     253      86      79      75      46      33      19
            
 
NaeI GCC'GGC

Cuts at:      0     568     780     870 
   Size:        568     212      90

  Fragments arranged by size: 

                 568     212      90
 
NarI GG'CG_CC

Cuts at:      0      14     870 
   Size:         14     856
 
NgoMIV G'CCGG_C

Cuts at:      0     566     778     870 
   Size:        566     212      92

  Fragments arranged by size: 

                 566     212      92
 
PasI CC'CwG_GG

Cuts at:      0     584     870 
   Size:        584     286
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     378     870 
   Size:        378     492
 
PfoI T'CCnGG_A

Cuts at:      0     764     870 
   Size:        764     106
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     870 
   Size:        343      32     495

  Fragments arranged by size: 

                 495     343      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     143     870 
   Size:        143     727
 
PspOMI G'GGCC_C

Cuts at:      0      30     205     587     870 
   Size:         30     175     382     283

  Fragments arranged by size: 

                 382     283     175      30
 
PvuII CAG'CTG

Cuts at:      0     339     414     739     818     851     870 
   Size:        339      75     325      79      33      19

  Fragments arranged by size: 

                 339     325      79      75      33      19
 
SfoI GGC'GCC

Cuts at:      0      15     870 
   Size:         15     855
 
SmaI CCC'GGG

Cuts at:      0     204     870 
   Size:        204     666
 
SrfI GCCC'GGGC

Cuts at:      0     204     870 
   Size:        204     666
 
StuI AGG'CCT

Cuts at:      0     526     626     870 
   Size:        526     100     244

  Fragments arranged by size: 

                 526     244     100
 
XmaI C'CCGG_G

Cuts at:      0     202     870 
   Size:        202     668
 

 Enzymes that do cut:

     AfeI   AflIII     AlfI     AloI    AlwNI     ApaI     AvaI     BanI
    BanII     BbeI    BbvCI     BcgI     BglI Bme1580I     BmrI     BpmI
   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BsiEI Bsp1286I
    BspMI    BsrBI    BsrFI   BssHII   BstEII    BstXI    BstYI   Bsu36I
    BtgZI     BtsI    CspCI     EaeI     EciI  Eco57MI    EcoNI EcoO109I
    HaeII     KasI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
   NgoMIV     PasI    PflMI     PfoI     PpiI    PpuMI   PspOMI    PvuII
     SfoI     SmaI     SrfI     StuI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI
     AhdI     AleI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BbsI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI    BpuEI    BsaBI    BsaWI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrDI    BsrGI
    BssSI   BstAPI    BstBI  BstZ17I     BtgI     ClaI     DraI   DraIII
     DrdI     EagI     EarI  EcoICRI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI     SmlI
    SnaBI     SpeI     SphI     SspI     StyI     SwaI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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