Restriction map for
pF1KB0996
(Linear) (Six-base) MAPSORT of: KIBB0996.seq Check: 6926 from: 1 to: 1395
KIBB0996.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 52 1395
Size: 52 1343
AccI GT'mk_AC
Cuts at: 0 630 1395
Size: 630 765
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 682 1395
Size: 460 222 713
Fragments arranged by size:
713 460 222
AlwNI CAG_nnn'CTG
Cuts at: 0 104 474 624 793 1227 1395
Size: 104 370 150 169 434 168
Fragments arranged by size:
434 370 169 168 150 104
ApoI r'AATT_y
Cuts at: 0 1238 1395
Size: 1238 157
BamHI G'GATC_C
Cuts at: 0 888 1395
Size: 888 507
BanI G'GyrC_C
Cuts at: 0 351 1395
Size: 351 1044
BanII G_rGCy'C
Cuts at: 0 192 718 1395
Size: 192 526 677
Fragments arranged by size:
677 526 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 395 458 1041 1395
Size: 395 63 583 354
Fragments arranged by size:
583 395 354 63
BciVI GTATCCnnnnn_n'
Cuts at: 0 943 1278 1395
Size: 943 335 117
Fragments arranged by size:
943 335 117
BlpI GC'TnA_GC
Cuts at: 0 717 1395
Size: 717 678
Bme1580I G_kGCm'C
Cuts at: 0 354 371 1114 1395
Size: 354 17 743 281
Fragments arranged by size:
743 354 281 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 174 1395
Size: 70 104 1221
Fragments arranged by size:
1221 104 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 813 1395
Size: 813 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 541 615 622 718 1395
Size: 403 138 74 7 96 677
Fragments arranged by size:
677 403 138 96 74 7
Bpu10I CC'TnA_GC
Cuts at: 0 58 1395
Size: 58 1337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 661 1395
Size: 661 734
BsaWI w'CCGG_w
Cuts at: 0 536 1069 1395
Size: 536 533 326
Fragments arranged by size:
536 533 326
BseYI C'CCAG_C
Cuts at: 0 228 1395
Size: 228 1167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1123 1395
Size: 1123 272
BsmBI CGTCTCn'nnnn_
Cuts at: 0 250 1395
Size: 250 1145
Bsp1286I G_dGCh'C
Cuts at: 0 192 354 371 718 1114 1395
Size: 192 162 17 347 396 281
Fragments arranged by size:
396 347 281 192 162 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 52 1395
Size: 52 1343
BsrBI CCG'CTC
Cuts at: 0 348 1395
Size: 348 1047
BsrDI GCAATG_nn'
Cuts at: 0 491 675 828 1395
Size: 491 184 153 567
Fragments arranged by size:
567 491 184 153
BssSI C'ACGA_G
Cuts at: 0 944 1395
Size: 944 451
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1227 1395
Size: 1227 168
BstYI r'GATC_y
Cuts at: 0 888 1395
Size: 888 507
Bsu36I CC'TnA_GG
Cuts at: 0 1029 1153 1395
Size: 1029 124 242
Fragments arranged by size:
1029 242 124
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 363 398 1395
Size: 363 35 997
Fragments arranged by size:
997 363 35
DraI TTT'AAA
Cuts at: 0 132 1395
Size: 132 1263
EarI CTCTTCn'nnn_
Cuts at: 0 572 658 1395
Size: 572 86 737
Fragments arranged by size:
737 572 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 460 541 615 622 682 718 1395
Size: 403 57 81 74 7 60 36 677
Fragments arranged by size:
677 403 81 74 60 57 36 7
EcoNI CCTnn'n_nnAGG
Cuts at: 0 571 1385 1395
Size: 571 814 10
Fragments arranged by size:
814 571 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 855 1338 1395
Size: 855 483 57
Fragments arranged by size:
855 483 57
HaeII r_GCGC'y
Cuts at: 0 342 1395
Size: 342 1053
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 518 550 873 905 1120 1152 1395
Size: 518 32 323 32 215 32 243
Fragments arranged by size:
518 323 243 215 32 32 32
HindIII A'AGCT_T
Cuts at: 0 832 1395
Size: 832 563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 237 1395
Size: 237 1158
MslI CAynn'nnrTG
Cuts at: 0 484 1117 1214 1395
Size: 484 633 97 181
Fragments arranged by size:
633 484 181 97
MspA1I CmG'CkG
Cuts at: 0 228 1395
Size: 228 1167
NdeI CA'TA_TG
Cuts at: 0 200 1395
Size: 200 1195
PflMI CCAn_nnn'nTGG
Cuts at: 0 690 1395
Size: 690 705
PfoI T'CCnGG_A
Cuts at: 0 689 979 1395
Size: 689 290 416
Fragments arranged by size:
689 416 290
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 902 934 1395
Size: 902 32 461
Fragments arranged by size:
902 461 32
PsiI TTA'TAA
Cuts at: 0 1277 1395
Size: 1277 118
PstI C_TGCA'G
Cuts at: 0 737 1395
Size: 737 658
PvuII CAG'CTG
Cuts at: 0 228 1395
Size: 228 1167
SapI GCTCTTCn'nnn_
Cuts at: 0 658 1395
Size: 658 737
SfcI C'TryA_G
Cuts at: 0 733 1395
Size: 733 662
SmlI C'TyrA_G
Cuts at: 0 676 1395
Size: 676 719
SspI AAT'ATT
Cuts at: 0 1363 1395
Size: 1363 32
StyI C'CwwG_G
Cuts at: 0 800 1395
Size: 800 595
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 970 1395
Size: 970 425
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 225 1395
Size: 27 198 1170
Fragments arranged by size:
1170 198 27
XmnI GAAnn'nnTTC
Cuts at: 0 914 1395
Size: 914 481
Enzymes that do cut:
AarI AccI AcuI AlwNI ApoI BamHI BanI BanII
BbsI BciVI BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaWI BseYI BsgI BsmBI Bsp1286I BspMI BsrBI
BsrDI BssSI BstAPI BstYI Bsu36I CspCI DraI EarI
Eco57MI EcoNI FalI HaeII Hin4I HindIII MmeI MslI
MspA1I NdeI PflMI PfoI PpiI PsiI PstI PvuII
SapI SfcI SmlI SspI StyI TaqII XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BclI BfrBI
BglI BglII BmgBI BmtI BsaI BsaAI BsaBI BsaHI
BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI BspEI BspHI
BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I BtgI
BtgZI BtsI ClaI DraIII DrdI EaeI EagI EciI
EcoICRI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpuMI PshAI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TatI TstI Tth111I
XbaI XhoI XmaI ZraI