Restriction map for pF1KB0993
 (Linear) (Six-base) MAPSORT of: KIBB0993.seq  Check: 4755  from: 1  to: 834



KIBB0993.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     616     834 
   Size:        196     420     218

  Fragments arranged by size: 

                 420     218     196
 
ApaI G_GGCC'C

Cuts at:      0      94     834 
   Size:         94     740
 
ApoI r'AATT_y

Cuts at:      0     571     834 
   Size:        571     263
 
AvaI C'yCGr_G

Cuts at:      0      93     772     834 
   Size:         93     679      62

  Fragments arranged by size: 

                 679      93      62
 
BanII G_rGCy'C

Cuts at:      0      20      94     833     834 
   Size:         20      74     739       1

  Fragments arranged by size: 

                 739      74      20       1
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      25     566     834 
   Size:         25     541     268

  Fragments arranged by size: 

                 541     268      25
 
BbvCI CC'TCA_GC

Cuts at:      0     110     834 
   Size:        110     724
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     448     482     834 
   Size:        448      34     352

  Fragments arranged by size: 

                 448     352      34
 
Bme1580I G_kGCm'C

Cuts at:      0      94     834 
   Size:         94     740
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132     834 
   Size:        132     702
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     275     834 
   Size:        275     559
 
Bpu10I CC'TnA_GC

Cuts at:      0      14     110     834 
   Size:         14      96     724

  Fragments arranged by size: 

                 724      96      14
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     469     775     834 
   Size:        469     306      59

  Fragments arranged by size: 

                 469     306      59
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     295     376     755     834 
   Size:        295      81     379      79

  Fragments arranged by size: 

                 379     295      81      79
 
BsaAI yAC'GTr

Cuts at:      0     591     834 
   Size:        591     243
 
BsaBI GATnn'nnATC

Cuts at:      0     436     834 
   Size:        436     398
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     163     193     834 
   Size:        163      30     641

  Fragments arranged by size: 

                 641     163      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     535     790     834 
   Size:        127     408     255      44

  Fragments arranged by size: 

                 408     255     127      44
 
BseYI C'CCAG_C

Cuts at:      0      87     136     744     766     834 
   Size:         87      49     608      22      68

  Fragments arranged by size: 

                 608      87      68      49      22
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     319     834 
   Size:        319     515
 
BsiHKAI G_wGCw'C

Cuts at:      0     833     834 
   Size:        833       1
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      94     833     834 
   Size:         20      74     739       1

  Fragments arranged by size: 

                 739      74      20       1
 
BsrFI r'CCGG_y

Cuts at:      0      75     196     349     834 
   Size:         75     121     153     485

  Fragments arranged by size: 

                 485     153     121      75
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     618     834 
   Size:        618     216
 
BtsI GCAGTG_nn'

Cuts at:      0     499     634     834 
   Size:        499     135     200

  Fragments arranged by size: 

                 499     200     135
 
ClaI AT'CG_AT

Cuts at:      0     360     834 
   Size:        360     474
 
EarI CTCTTCn'nnn_

Cuts at:      0     506     679     834 
   Size:        506     173     155

  Fragments arranged by size: 

                 506     173     155
 
EcoICRI GAG'CTC

Cuts at:      0     831     834 
   Size:        831       3
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     196     275     616     834 
   Size:        196      79     341     218

  Fragments arranged by size: 

                 341     218     196      79
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     337     834 
   Size:        337     497
 
EcoRI G'AATT_C

Cuts at:      0     571     834 
   Size:        571     263
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     201     294     834 
   Size:        201      93     540

  Fragments arranged by size: 

                 540     201      93
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       7      39     163     195     616     648     834 
   Size:          7      32     124      32     421      32     186

  Fragments arranged by size: 

                 421     186     124      32      32      32       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     834 
   Size:        254     580
 
MspA1I CmG'CkG

Cuts at:      0      29     655     834 
   Size:         29     626     179

  Fragments arranged by size: 

                 626     179      29
 
NaeI GCC'GGC

Cuts at:      0      77     834 
   Size:         77     757
 
NgoMIV G'CCGG_C

Cuts at:      0      75     834 
   Size:         75     759
 
NspI r_CATG'y

Cuts at:      0     378     834 
   Size:        378     456
 
PasI CC'CwG_GG

Cuts at:      0     660     834 
   Size:        660     174
 
PfoI T'CCnGG_A

Cuts at:      0     236     320     834 
   Size:        236      84     514

  Fragments arranged by size: 

                 514     236      84
 
PmlI CAC'GTG

Cuts at:      0     591     834 
   Size:        591     243
 
PspOMI G'GGCC_C

Cuts at:      0      90     834 
   Size:         90     744
 
PstI C_TGCA'G

Cuts at:      0     471     834 
   Size:        471     363
 
PvuII CAG'CTG

Cuts at:      0     655     834 
   Size:        655     179
 
SacI G_AGCT'C

Cuts at:      0     833     834 
   Size:        833       1
 
SapI GCTCTTCn'nnn_

Cuts at:      0     679     834 
   Size:        679     155
 
SexAI A'CCwGG_T

Cuts at:      0     404     834 
   Size:        404     430
 
SfcI C'TryA_G

Cuts at:      0     467     834 
   Size:        467     367
 
SmlI C'TyrA_G

Cuts at:      0     484     754     834 
   Size:        484     270      80

  Fragments arranged by size: 

                 484     270      80
 
StuI AGG'CCT

Cuts at:      0     250     834 
   Size:        250     584
 
StyI C'CwwG_G

Cuts at:      0     206     368     698     834 
   Size:        206     162     330     136

  Fragments arranged by size: 

                 330     206     162     136
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     736     834 
   Size:        736      98
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     250     834 
   Size:        250     584
 

 Enzymes that do cut:

     AcuI     ApaI     ApoI     AvaI    BanII     BbsI    BbvCI     BcgI
 Bme1580I     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BsrFI    BstXI
     BtsI     ClaI     EarI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI
    Hin4I     MmeI   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI
     PmlI   PspOMI     PstI    PvuII     SacI     SapI    SexAI     SfcI
     SmlI     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI EcoO109I    EcoRV     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     MslI     NarI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PpiI
    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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