Restriction map for pF1KB0979
 (Linear) (Six-base) MAPSORT of: KIBB0979.seq  Check: 5926  from: 1  to: 690



KIBB0979.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     647     690 
   Size:        392     255      43

  Fragments arranged by size: 

                 392     255      43
 
AflIII A'CryG_T

Cuts at:      0      66     690 
   Size:         66     624
 
ApoI r'AATT_y

Cuts at:      0      11     109     294     690 
   Size:         11      98     185     396

  Fragments arranged by size: 

                 396     185      98      11
 
BanII G_rGCy'C

Cuts at:      0     364     690 
   Size:        364     326
 
BglI GCCn_nnn'nGGC

Cuts at:      0     684     690 
   Size:        684       6
 
BlpI GC'TnA_GC

Cuts at:      0     128     690 
   Size:        128     562
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     108     690 
   Size:        108     582
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     608     690 
   Size:        608      82
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     469     472     690 
   Size:        469       3     218

  Fragments arranged by size: 

                 469     218       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     364     510     690 
   Size:        364     146     180

  Fragments arranged by size: 

                 364     180     146
 
Bsp1286I G_dGCh'C

Cuts at:      0     364     510     690 
   Size:        364     146     180

  Fragments arranged by size: 

                 364     180     146
 
BspHI T'CATG_A

Cuts at:      0     290     690 
   Size:        290     400
 
BtsI GCAGTG_nn'

Cuts at:      0     270     690 
   Size:        270     420
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     246     281     690 
   Size:        246      35     409

  Fragments arranged by size: 

                 409     246      35
 
EaeI y'GGCC_r

Cuts at:      0     162     690 
   Size:        162     528
 
EcoICRI GAG'CTC

Cuts at:      0     362     690 
   Size:        362     328
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     608     647     690 
   Size:        392     216      39      43

  Fragments arranged by size: 

                 392     216      43      39
 
EcoRV GAT'ATC

Cuts at:      0     327     690 
   Size:        327     363
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     130     690 
   Size:        130     560
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     373     405     690 
   Size:        373      32     285

  Fragments arranged by size: 

                 373     285      32
 
MscI TGG'CCA

Cuts at:      0     164     690 
   Size:        164     526
 
MspA1I CmG'CkG

Cuts at:      0     444     690 
   Size:        444     246
 
NspI r_CATG'y

Cuts at:      0      70     690 
   Size:         70     620
 
PciI A'CATG_T

Cuts at:      0      66     690 
   Size:         66     624
 
PsiI TTA'TAA

Cuts at:      0     320     690 
   Size:        320     370
 
PvuII CAG'CTG

Cuts at:      0     444     690 
   Size:        444     246
 
SacI G_AGCT'C

Cuts at:      0     364     690 
   Size:        364     326
 
SfcI C'TryA_G

Cuts at:      0     577     690 
   Size:        577     113
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     463     594     636     690 
   Size:        463     131      42      54

  Fragments arranged by size: 

                 463     131      54      42
 
XmnI GAAnn'nnTTC

Cuts at:      0     110     690 
   Size:        110     580
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BanII     BglI     BlpI     BplI     BpmI
    BseRI  BsiHKAI Bsp1286I    BspHI     BtsI    CspCI     EaeI  EcoICRI
  Eco57MI    EcoRV     FalI    Hin4I     MscI   MspA1I     NspI     PciI
     PsiI    PvuII     SacI     SfcI    TaqII     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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